Drosophila Rolling pebbles

Menon, Sree Devi; Chia, William

doi:10.1016/s1534-5807(01)00075-2

Cited by 111 publications

(38 citation statements)

References 46 publications

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“…In rols mutants syncytial longitudinal muscle development was disturbed, and we found mostly binucleated mini-muscles. The first fusion events of the longitudinal FCs might function without Rols7, analogous to the first phase of fusion in the somatic mesoderm, which is independent of Rols7 function [40,41,43]. In longitudinal visceral fusion, the adaptor protein Rols7 might bind to Duf/Kirre to allow efficient fusion via a positive feedback loop, as is the case in the somatic mesoderm [68].…”

Section: Discussionmentioning

confidence: 99%

Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

et al. 2014

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BackgroundThe visceral musculature of Drosophila larvae comprises circular visceral muscles tightly interwoven with longitudinal visceral muscles. During myogenesis, the circular muscles arise by one-to-one fusion of a circular visceral founder cell (FC) with a visceral fusion-competent myoblast (FCM) from the trunk visceral mesoderm, and longitudinal muscles arise from FCs of the caudal visceral mesoderm. Longitudinal FCs migrate anteriorly under guidance of fibroblast growth factors during embryogenesis; it is proposed that they fuse with FCMs from the trunk visceral mesoderm to give rise to syncytia containing up to six nuclei.ResultsUsing fluorescence in situ hybridization and immunochemical analyses, we investigated whether these fusion events during migration use the same molecular repertoire and cellular components as fusion-restricted myogenic adhesive structure (FuRMAS), the adhesive signaling center that mediates myoblast fusion in the somatic mesoderm. Longitudinal muscles were formed by the fusion of one FC with Sns-positive FCMs, and defects in FCM specification led to defects in longitudinal muscle formation. At the fusion sites, Duf/Kirre and the adaptor protein Rols7 accumulated in longitudinal FCs, and Blow and F-actin accumulated in FCMs. The accumulation of these four proteins at the fusion sites argues for FuRMAS-like adhesion and signaling centers. Longitudinal fusion was disturbed in rols and blow single, and scar wip double mutants. Mutants of wasp or its interaction partner wip had no defects in longitudinal fusion.ConclusionsOur results indicated that all embryonic fusion events depend on the same cell-adhesion molecules, but that the need for Rols7 and regulators of F-actin distinctly differs. Rols7 was required for longitudinal visceral and somatic myoblast fusion but not for circular visceral fusion. Importantly, longitudinal fusion depended on Kette and SCAR/Wave but was independent of WASp-dependent Arp2/3 activation. Thus, the complexity of the players involved in muscle formation increases from binucleated circular muscles to longitudinal visceral muscles to somatic muscles.

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Section: Discussionmentioning

confidence: 99%

Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

et al. 2014

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“…Other fly stocks used include: mib1 EY9780 (Pitsouli and Delidakis, 2005), UAS-neur (Pavlopoulos et al, 2001), UAS-mib1-3 (Wang and Struhl, 2005), rP298-Gal4 (Menon and Chia, 2001), and ILK-GFP (Zervas et al, 2001). …”

Section: Methodsmentioning

confidence: 99%

Drosophila mind bomb2is required for maintaining muscle integrity and survival

Nguyen

Voza

Ezzeddine

et al. 2007

The Journal of Cell Biology

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We report that the Drosophila mind bomb2 (mib2) gene is a novel regulator of muscle development. Unlike its paralogue, mib1, zygotic expression of mib2 is restricted to somatic and visceral muscle progenitors, and their respective differentiated musculatures. We demonstrate that in embryos that lack functional Mib2, muscle detachment is observed beginning in mid stage 15 and progresses rapidly, culminating in catastrophic degeneration and loss of most somatic muscles by stage 17. Notably, the degenerating muscles are positive for apoptosis markers, and inhibition of apoptosis in muscles prevents to a significant degree the muscle defects. Rescue experiments with Mib1 and Neuralized show further that these E3 ubiquitin ligases are not capable of ameliorating the muscle mutant phenotype of mib2. Our data suggest strongly that mib2 is involved in a novel Notch- and integrin-independent pathway that maintains the integrity of fully differentiated muscles and prevents their apoptotic degeneration.

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“…The molecular composition of these vesicles requires future investigation. Another piece of evidence to support the involvement of vesicle trafficking in myoblast fusion is the finding that a founder cell-specific adaptor protein, Antisocial/Rolling pebbles (Ants/Rols) [55,57,58], functions to replenish the cell adhesion molecule Duf by translocating Duf in exocytic vesicles [59]. Moreover, genetics studies have implicated Singles bar (Sing), a MARVEL domain-containing four-pass transmembrane protein, in myoblast fusion [56] (Figure 2).…”

Section: Other Cellular Processes During Myoblast Fusionmentioning

confidence: 99%

Mechanisms of myoblast fusion during muscle development

Kim

Jin

Duan

et al. 2015

Current Opinion in Genetics & Development

215

181

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The development and regeneration of skeletal muscles require the fusion of mononulceated muscle cells to form multinucleated, contractile muscle fibers. Studies using a simple genetic model, Drosophila melanogaster, have discovered many evolutionarily conserved fusion-promoting factors in vivo. Recent work in zebrafish and mouse also identified several vertebrate-specific factors required for myoblast fusion. Here, we integrate progress in multiple in vivo systems and highlight conceptual advance in understanding how muscle cell membranes are brought together for fusion. We focus on the molecular machinery at the fusogenic synapse and present a three-step model to describe the molecular and cellular events leading to fusion pore formation.

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Drosophila Rolling pebbles

Cited by 111 publications

References 46 publications

Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

Drosophila mind bomb2is required for maintaining muscle integrity and survival

Mechanisms of myoblast fusion during muscle development

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