Drosophila microRNAs 263a/b Confer Robustness during Development by Protecting Nascent Sense Organs from Apoptosis

Hilgers, Valérie; Bushati, Natascha; Cohen, Stephen M.

doi:10.1371/journal.pbio.1000396

Cited by 97 publications

(97 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, the expression of many miRNAs is developmentally regulated, consistent with roles for miRNAs in the regulation of gene expression in conjunction with developmental processes (Bartel 2004;Kato and Slack 2008). Furthermore, roles for miRNAs in providing robustness of developmental outcomes to various perturbations have been demonstrated (Li et al 2009;Hilgers et al 2010;Rasmussen et al 2010).…”

Section: Introductionmentioning

confidence: 72%

Effect of life history on microRNA expression during C. elegans development

Karp¹,

Hammell²,

Ow³

et al. 2011

RNA

View full text Add to dashboard Cite

Animals have evolved mechanisms to ensure the robustness of developmental outcomes to changing environments. MicroRNA expression may contribute to developmental robustness because microRNAs are key post-transcriptional regulators of developmental gene expression and can affect the expression of multiple target genes. Caenorhabditis elegans provides an excellent model to study developmental responses to environmental conditions. In favorable environments, C. elegans larvae develop rapidly and continuously through four larval stages. In contrast, in unfavorable conditions, larval development may be interrupted at either of two diapause stages: The L1 diapause occurs when embryos hatch in the absence of food, and the dauer diapause occurs after the second larval stage in response to environmental stimuli encountered during the first two larval stages. Dauer larvae are stress resistant and long lived, permitting survival in harsh conditions. When environmental conditions improve, dauer larvae re-enter development, and progress through two post-dauer larval stages to adulthood. Strikingly, all of these life history options (whether continuous or interrupted) involve an identical pattern and sequence of cell division and cell fates. To identify microRNAs with potential functions in buffering development in the context of C. elegans life history options, we used multiplex real-time PCR to assess the expression of 107 microRNAs throughout development in both continuous and interrupted life histories. We identified 17 microRNAs whose developmental profile of expression is affected by dauer life history and/or L1 diapause, compared to continuous development. Hence these microRNAs could function to regulate gene expression programs appropriate for different life history options in the developing worm.

show abstract

Section: Introductionmentioning

confidence: 72%

Effect of life history on microRNA expression during C. elegans development

Karp¹,

Hammell²,

Ow³

et al. 2011

RNA

View full text Add to dashboard Cite

show abstract

“…This type of threshold might be important not only in circadian cell determination but also to prevent stochastic or random pulses of CLK-driven activity that could alter or shift the circadian clock. miRNA-mediated thresholding of master developmental regulators has been previously demonstrated for the miR-9a/senseless 61 and for the miR-263a/hid pairs 62 . We believe that our failure to detect signal from the Clk 3 0 UTR reporters is not related to a sensitivity issue.…”

Section: Discussionmentioning

confidence: 94%

Clk post-transcriptional control denoises circadian transcription both temporally and spatially

et al. 2015

View full text Add to dashboard Cite

The transcription factor CLOCK (CLK) is essential for the development and maintenance of circadian rhythms in Drosophila. However, little is known about how CLK levels are controlled. Here we show that Clk mRNA is strongly regulated post-transcriptionally through its 3 0 UTR. Flies expressing Clk transgenes without normal 3 0 UTR exhibit variable CLK-driven transcription and circadian behaviour as well as ectopic expression of CLK-target genes in the brain. In these flies, the number of the key circadian neurons differs stochastically between individuals and within the two hemispheres of the same brain. Moreover, flies carrying Clk transgenes with deletions in the binding sites for the miRNA bantam have stochastic number of pacemaker neurons, suggesting that this miRNA mediates the deterministic expression of CLK. Overall our results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription, which is essential for proper development and maintenance of circadian rhythms in Drosophila.

show abstract

“…In total, 18 mutants showed significantly reduced viability (p < 0.01, binomial test, summarized in Table 2). These include bantam, miR-1, the let-7 (miR-100, miR-125) cluster, miR-8, the miR-309 cluster, miR-263a and miR-276a, which have previously been reported to reduce viability (Brennecke et al, 2003;Bushati et al, 2008;Caygill and Johnston, 2008;Ge et al, 2012;Hilgers et al, 2010;Karres et al, 2007;Sokol and Ambros, 2005;Li et al, 2013), as well as several miRNAs for which no viability data have been reported previously.…”

Section: Survival Phenotypesmentioning

confidence: 99%

Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations

et al. 2014

View full text Add to dashboard Cite

MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

show abstract

Drosophila microRNAs 263a/b Confer Robustness during Development by Protecting Nascent Sense Organs from Apoptosis

Abstract: microRNA-263a/b confer robustness to sense organ development by controlling expression of the pro-apoptotic gene hid during apoptotic tissue pruning in Drosophila.

Cited by 97 publications

References 62 publications

Effect of life history on microRNA expression during C. elegans development

Effect of life history on microRNA expression during C. elegans development

Clk post-transcriptional control denoises circadian transcription both temporally and spatially

Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations

Contact Info

Product

Resources

About