DROSHA-Dependent miRNA and AIM2 Inflammasome Activation in Idiopathic Pulmonary Fibrosis

Cho, Soo Jung; Lee, Mihye; Stout‐Delgado, Heather; Moon, Jong‐Seok

doi:10.3390/ijms21051668

Cited by 14 publications

(14 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, besides analyzing protein levels, in future studies we will also analyze miRNAs that have been shown to affect inflammasome signaling (Wang et al, 2009;Pan et al, 2018;Cho et al, 2020) since miRNAs in extracellular vesicles have been recently shown to be useful biomarkers of psoriasis (Wang et al, 2020), and we have shown that inflammasome proteins in extracellular vesicles are good biomarkers of stroke (Kerr et al, 2018a). For instance, silencing of miR-155 is able to downregulate inflammasome signaling in Ps; thus, suggesting that miRNAs play an important role in Ps (Luo et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

The Inflammasome Signaling Proteins ASC and IL-18 as Biomarkers of Psoriasis

et al. 2020

View full text Add to dashboard Cite

Inflammasome activation in the innate immune response plays a role in the pathogenesis of psoriasis largely due to the increased levels of pro-inflammatory cytokines. However, the precise role of inflammasomes in psoriasis (Ps) and psoriatic arthritis (PsA) is largely undefined. To establish the reliability of inflammasome signaling proteins as diagnostics and predictive biomarkers of clinical severity in this disease population, serum from healthy donors and patients with Ps/PsA were analyzed for the protein expression of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), interleukin (IL)-1b and IL-18 levels to determine cutoff points, positive and negative predictive values, and receiver operator characteristic (ROC) curves. Our data revealed that ASC and IL-18 proteins were significantly higher in the Ps group when compared to healthy controls. The area under the curve (AUC) for ASC was 0.9224 with a cutoff point of 321.8 pg/ml, while IL-18 had an AUC of 0.7818 and a cutoff point of 232.1 pg/ml. In addition, levels of IL-18 had a statistically significant linear correlation with that of ASC with an adjusted R squared of 0.2566, indicating that approximately 25% of IL-18 levels could be explained by ASC levels in serum. Our findings indicate that ASC and IL-18 play a significant role in the inflammatory response associated with the pathology of Ps. These inflammasome proteins appear to be key biomarkers in determining diagnoses in this patient population.

show abstract

Section: Discussionmentioning

confidence: 99%

The Inflammasome Signaling Proteins ASC and IL-18 as Biomarkers of Psoriasis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…miRNAs, a class of noncoding small RNAs, are involved in RNA silencing, posttranscriptional regulation, and other biological processes [20]. It has been confirmed that miRNAs play a critical role in the occurrence and development of various diseases, including IPF [21][22][23]. As an example, miR-92, miR-210, and miR-let-7d have been confirmed to be associated with IPF [24][25][26].…”

Section: Introductionmentioning

confidence: 98%

[Retracted] Identification and Validation of Potential Biomarkers and Pathways for Idiopathic Pulmonary Fibrosis by Comprehensive Bioinformatics Analysis

Qian

Cai

Qian

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Objective. Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible, high-mortality lung disease, but its pathogenesis is still unclear. Our purpose was to explore potential genes and molecular mechanisms underlying IPF. Methods. IPF-related data were obtained from the GSE99621 dataset. Differentially expressed genes (DEGs) were identified between IPF and controls. Their biological functions were analyzed. The relationships between DEGs and microRNAs (miRNAs) were predicted. DEGs and pathways were validated in a microarray dataset. A protein-protein interaction (PPI) network was constructed based on these common DEGs. Western blot was used to validate hub genes in IPF cell models by western blot. Results. DEGs were identified for IPF than controls in the RNA-seq dataset. Functional enrichment analysis showed that these DEGs were mainly enriched in immune and inflammatory response, chemokine-mediated signaling pathway, cell adhesion, and other biological processes. In the miRNA-target network based on RNA-seq dataset, we found several miRNA targets among all DEGs, like RAB11FIP1, TGFBR3, and SPP1. We identified 304 upregulated genes and 282 downregulated genes in IPF compared to controls both in the microarray and RNA-seq datasets. These common DEGs were mainly involved in cell adhesion, extracellular matrix organization, oxidation-reduction process, and lung vasculature development. In the PPI network, 3 upregulated and 4 downregulated genes could be considered hub genes, which were confirmed in the IPF cell models. Conclusion. Our study identified several IPF-related DEGs that could become potential biomarkers for IPF. Large-scale multicentric studies are eagerly needed to confirm the utility of these biomarkers.

show abstract

“…Once transcription is completed, pri-miRNAs are processed into premature miRNAs (pre-miRNAs) which are stem–loop structures of about 80 nucleotides in length. The processing of pri-miRNAs to pre-miRNAs is fulfilled by a microprocessor complex, which consists of Drosha, a double-stranded RNA-specific ribonuclease (RNase) III [ 1 , 2 , 11 , 12 ], and DGCR8, a molecular anchor recognizing the double-strand/single-strand junction of pri-miRNAs [ 13 ]. With the help of other additional factors, such as DDX5 and DDX17 RNA helicases [ 14 ], the Drosha:DGCR8 complex cuts out the stem–loop structure (pre-miRNA) from the pri-miRNA at the precise positions on the 5′ and 3′ sides.…”

Section: Biogenesis Of Mirnasmentioning

confidence: 99%

Diagnostic and Therapeutic Implications of microRNAs in Non-Small Cell Lung Cancer

Ahn

2020

IJMS

View full text Add to dashboard Cite

microRNAs (miRNAs), endogenous suppressors of target mRNAs, are deeply involved in every step of non-small cell lung cancer (NSCLC) development, from tumor initiation to progression and metastasis. They play roles in cell proliferation, apoptosis, angiogenesis, epithelial-to-mesenchymal transition, migration, invasion, and metastatic colonization, as well as immunosuppression. Due to their versatility, numerous attempts have been made to use miRNAs for clinical applications. miRNAs can be used as cancer subtype classifiers, diagnostic markers, drug-response predictors, prognostic markers, and therapeutic targets in NSCLC. Many challenges remain ahead of their actual clinical application; however, when achieved, the use of miRNAs in the clinic is expected to enable great progress in the diagnosis and treatment of patients with NSCLC.

show abstract

DROSHA-Dependent miRNA and AIM2 Inflammasome Activation in Idiopathic Pulmonary Fibrosis

Cited by 14 publications

References 131 publications

The Inflammasome Signaling Proteins ASC and IL-18 as Biomarkers of Psoriasis

The Inflammasome Signaling Proteins ASC and IL-18 as Biomarkers of Psoriasis

[Retracted] Identification and Validation of Potential Biomarkers and Pathways for Idiopathic Pulmonary Fibrosis by Comprehensive Bioinformatics Analysis

Diagnostic and Therapeutic Implications of microRNAs in Non-Small Cell Lung Cancer

Contact Info

Product

Resources

About