Droplet Microfluidic Technique for the Study of Fermentation

Churski, Krzysztof; Ruszczak, Artur; Jakieła, Sławomir; Garstecki, Piotr

doi:10.3390/mi6101435

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…103 Droplet microfluidic assays have also been developed for estimating the alcohol production rate of microbes using enzymatic assays. 104,105 Multiple fluorescent markers can be detected/monitored simultaneously in droplet cultures. Shim et al presented a microfluidic device that was capable of trapping and storing picoliter droplets.…”

Section: Applications Of Droplet Microfluidics In Microbiologymentioning

confidence: 99%

“…[179][180][181] However, the high elasticity of PDMS makes it difficult to precisely control the flow of long sequences of droplets due to slow flow caused by relaxation of pressure. Recent approaches proposed the use of chips and tubing made of stiff materials such as polycarbonate, 7,105,113 polyĲtetrafluoroethylene) (PTFE, Teflon) 100,112 or fluorinated ethylene propylene (FEP). 5,6 New methods of fabrication and modification of stiff polymers should be developed for systems for long-term culturing of bacteria without wetting or excessive emulsification.…”

Section: Wetting and Cross-contaminationmentioning

confidence: 99%

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Droplet microfluidics for microbiology: techniques, applications and challenges

2016

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Droplet microfluidics has rapidly emerged as one of the key technologies opening up new experimental possibilities in microbiology. The ability to generate, manipulate and monitor droplets carrying single cells or small populations of bacteria in a highly parallel and high throughput manner creates new approaches for solving problems in diagnostics and for research on bacterial evolution. This review presents applications of droplet microfluidics in various fields of microbiology: i) detection and identification of pathogens, ii) antibiotic susceptibility testing, iii) studies of microbial physiology and iv) biotechnological selection and improvement of strains. We also list the challenges in the dynamically developing field and new potential uses of droplets in microbiology.

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Section: Applications Of Droplet Microfluidics In Microbiologymentioning

confidence: 99%

Section: Wetting and Cross-contaminationmentioning

confidence: 99%

Droplet microfluidics for microbiology: techniques, applications and challenges

2016

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“…The proposed method was successfully applied to the analysis of 12 distinct categories of alcoholic drinks with ethanol concentrations ranging from 5% v/v for beer to 53 percent v/v for scotch whiskey. Churski et al [92] demonstrated a microfluidic platform for determining the concentration of ethanol in micro-droplets (Fig. 2C).…”

Section: Ethanol Concentrationmentioning

confidence: 99%

Microfluidics and the Spirits Industry: A Review

Mondal,

Williams

2023

JDS

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There is and will always be the need to provide authentic and safe alcoholic beverages. To achieve this goal, researchers are constantly looking for innovative and practical approaches to analyze their samples and control their quality. Quality control can be challenging in the spirits industry due to their production (mashing, fermenting, distilling, aging, packaging) and their complex chemical composition. To assess the quality and safety of alcoholic beverages, a variety of analytical procedures have been developed and are currently in use. Although these laboratory-based approaches offer a high level of sensitivity, they can be both time-consuming and costly. Therefore, it is an ongoing need to advance simpler, faster, more precise, field-deployable, and more sensitive instruments to identify chemicals and abnormalities in alcoholic beverages. We believe many needs can be addressed using microfluidic technologies, sometimes referred to as lab-on-a-chip devices. In this review, we will discuss recent significant advancements in microfluidics for the assessment of alcoholic beverage products. The analysis and viewpoints presented in this study are intended to stimulate continued development of microfluidic devices in the spirits sector and other food safety testing and monitoring fields, benefiting human health and overall well-being.

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“…The sensitivity of this device is higher than that of breathalyzers, and the device can work in a wide ROD of 0.0005-0.6 g/L. Droplet microdroplet screening is utilized to determine ethanol levels in cell cultures (Scheme 20 B) [461]. The prototype is based on an electrochemical alcohol oxidase/HRP sensor, and it provides a useful device for detecting the alcohol consumption of drivers.…”

Section: Scheme 19mentioning

confidence: 99%