Droplet‐based electrospray ionization mass spectrometry for qualitative and quantitative analysis

Wong, Song Yi; Chen, Yu-Chie

doi:10.1002/jms.3355

Cited by 6 publications

(9 citation statements)

References 33 publications

(42 reference statements)

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“…Nevertheless, the gas‐phase ions with a wide mass range were observed by the mass spectrometer. Several other methods, employing the same principle have also been reported recently . Thus, we believed that a static droplet can function as an ESI source and generate finer droplets for ionization when a suitable setup is established.…”

Section: Introductionmentioning

confidence: 90%

“…High electric field applied in the mass spectrometer can be used to induce polarization of sample droplets without direct application of a high electric voltage on the sample emitter. As a result, analyte ions in gas phase can be generated via polarization induced ESI processes . For example, in ultrasonication‐assisted spray ionization MS, ultrasonic transducers were used to generate gas‐phase ions from liquid samples in the proximity of a mass spectrometer.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, analyte ions in gas phase can be generated via polarization induced ESI processes. [14][15][16][17][18][19][20][21][22][23] For example, in ultrasonication-assisted spray ionization MS, [14][15][16][17] ultrasonic transducers were used to generate gas-phase ions from liquid samples in the proximity of a mass spectrometer. Although no direct electric-contact was applied in the sample inlet, polarization induced by the electric field provided by the mass spectrometer was involved in the ionization processes of these approaches.…”

Section: Introductionmentioning

confidence: 99%

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Polarization induced electrospray ionization mass spectrometry for the analysis of liquid, viscous and solid samples

Meher

Chen

2015

J. Mass Spectrom.

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In this study, a polarization-induced electrospray ionization mass spectrometry (ESI-MS) was developed. A micro-sized sample droplet was deposited on a naturally available dielectric substrate such as a fruit or a stone, and then placed close to (~2 mm) the orifice of a mass spectrometer applied with a high voltage. Taylor cone was observed from the sample droplet, and a spray emitted from the cone apex was generated. The analyte ion signals derived from the droplet were obtained by the mass spectrometer. The ionization process is similar to that in ESI although no direct electric contact was applied on the sample site. The sample droplet polarized by the high electric field provided by the mass spectrometer initiated the ionization process. The dielectric sample loading substrate facilitated further the polarization process, resulting in the formation of Taylor cone. The mass spectral profiles obtained via this approach resembled those obtained using ESI-MS. Multiply charged ions dominated the mass spectra of peptides and proteins, whereas singly charged ions dominated the mass spectra of small molecules such as amino acids and small organic molecules. In addition to liquid samples, this approach can be used for the analysis of solid and viscous samples. A small droplet containing suitable solvent (5-10 µl) was directly deposited on the surface of the solid (or viscous) sample, placed close the orifice of mass spectrometer applied with a high voltage. Taylor cone derived from the droplet was immediately formed followed by electrospray processes to generate gas-phase ions for MS analysis. Analyte ions derived from the main ingredients of pharmaceutical tablets and viscous ointment can be extracted into the solvent droplet in situ and observed using a mass spectrometer.

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Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polarization induced electrospray ionization mass spectrometry for the analysis of liquid, viscous and solid samples

Meher

Chen

2015

J. Mass Spectrom.

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“…Microdroplets from an automatic pipette at regular time intervals also gave rise to signal MS, speci cally when positioned in proximity to the MS inlet. 87) e droplet from the pipette experienced the polarization e ect due to high voltage at MS inlet. e pressure di erence at the same point allowed the droplet to enter MS ori ce, leading to generation of gas-phase ions.…”

Section: )mentioning

confidence: 99%

“…e pressure di erence at the same point allowed the droplet to enter MS ori ce, leading to generation of gas-phase ions. 87) Recently, another method, called as polarization induced electrospray ionization (PI-ESI), 88) was reported by the same group. Electrospray can be directly generated from a microliter-size droplets (4-10 µL) that is placed over a dielectric material (e.g., a cherry tomato), which is placed close to the inlet of a mass spectrometer, applied with a high voltage (Fig.…”

Section: )mentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

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Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

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