Drop-on-demand cell bioprinting via Laser Induced Side Transfer (LIST)

Orimi, Hamid Ebrahimi; Kolkooh, Sayadeh Sara Hosseini; Hooker, Erika; Narayanswamy, Sivakumar; Larrivée, Bruno; Boutopoulos, Christos

doi:10.1038/s41598-020-66565-x

Cited by 24 publications

(10 citation statements)

References 29 publications

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“…For this, neuron-laden droplets were printed using two laser energies (100 and 120 μJ) ( Figure 1 ). The selection of the laser energies was based on our previous work on LIST [ 13 ]. After printing, the cells were cultured for 2 days and the cell viability was assessed ( Figure 2 A–L).…”

Section: Resultsmentioning

confidence: 99%

“…Printing patterns consisted of arrays of individual droplets separated by a 500 μm gap. A detailed description of the printing setup can be found in our previous work [ 13 ]. After printing, the samples were placed in an incubator for 20 min, then rinsed twice with Neurobasal-A medium, completed with 2 mL of neurobasal-A medium, and put back in the incubator for 48 h.…”

Section: Methodsmentioning

confidence: 99%

“…By using a nozzleless approach, LIFT is compatible with bioinks having a wide viscosity range (1–300 mPa·s). However, 3D printing capabilities remain limited in LIFT due to unresolved donor preparation challenges [ 13 ]. A modified methodology for the laser bioprinting of cells, called Laser Induced Side Transfer (LIST), was recently developed by our group [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, 3D printing capabilities remain limited in LIFT due to unresolved donor preparation challenges [ 13 ]. A modified methodology for the laser bioprinting of cells, called Laser Induced Side Transfer (LIST), was recently developed by our group [ 13 ]. LIST uses low energy nanosecond laser pulses to generate a transient microbubble at the distal end of a glass microcapillary supplied with bioink.…”

Section: Introductionmentioning

confidence: 99%

“…Microbubble expansion results in the ejection of a cell-laden microjet perpendicular to the irradiation axis. We previously showed that LIST-printed human umbilical vein endothelial cells (HUVECs) present negligible loss of viability, and maintain their abilities to migrate, proliferate and form intercellular junctions [ 13 ]. This method is technically uncomplicated and aims to cover a technological gap in the drop-on-demand bioprinting field: the lack of technologies that can 3D print large-scale constructs using both high and low viscosity bioinks.…”

Section: Introductionmentioning

confidence: 99%

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Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)

et al. 2021

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Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG; cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)

et al. 2021

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Shining a Light on Cancer—Photonics in Microfluidic Tumor Modeling and Biosensing

Guimarães

Cruz‐Moreira

Caballero

et al. 2022

Adv Healthcare Materials

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Microfluidic platforms represent a powerful approach to miniaturizing important characteristics of cancers, improving in vitro testing by increasing physiological relevance. Different tools can manipulate cells and materials at the microscale, but few offer the efficiency and versatility of light and optical technologies. Moreover, light‐driven technologies englobe a broad toolbox for quantifying critical biological phenomena. Herein, the role of photonics in microfluidic 3D cancer modeling and biosensing from three major perspectives is reviewed. First, optical‐driven technologies are looked upon, as these allow biomaterials and living cells to be manipulated with microsized precision and present opportunities to advance 3D microfluidic models by engineering cancer microenvironments' hallmarks, such as their architecture, cellular complexity, and vascularization. Second, the growing field of optofluidics is discussed, exploring how optical tools can directly interface microfluidic chips, enabling the extraction of relevant biological data, from single fluorescent signals to the complete 3D imaging of diseased cells within microchannels. Third, advances in optical cancer biosensing are reviewed, focusing on how light–matter interactions can detect biomarkers, rare circulating tumor cells, and cell‐derived structures such as exosomes. Photonic technologies' current challenges and caveats in microfluidic 3D cancer models are overviewed, outlining future research avenues that may catapult the field.

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Bioprinted research models of urological malignancy

Wang,

Mao,

Wang

et al. 2024

Exploration

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Urological malignancy (UM) is among the leading threats to health care worldwide. Recent years have seen much investment in fundamental UM research, including mechanistic investigation, early diagnosis, immunotherapy, and nanomedicine. However, the results are not fully satisfactory. Bioprinted research models (BRMs) with programmed spatial structures and functions can serve as powerful research tools and are likely to disrupt traditional UM research paradigms. Herein, a comprehensive review of BRMs of UM is presented. It begins with a brief introduction and comparison of existing UM research models, emphasizing the advantages of BRMs, such as modeling real tissues and organs. Six kinds of mainstream bioprinting techniques used to fabricate such BRMs are summarized with examples. Thereafter, research advances in the applications of UM BRMs, such as culturing tumor spheroids and organoids, modeling cancer metastasis, mimicking the tumor microenvironment, constructing organ chips for drug screening, and isolating circulating tumor cells, are comprehensively discussed. At the end of this review, current challenges and future development directions of BRMs and UM are highlighted from the perspective of interdisciplinary science.

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Drop-on-demand cell bioprinting via Laser Induced Side Transfer (LIST)

Cited by 24 publications

References 29 publications

Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)

Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)

Shining a Light on Cancer—Photonics in Microfluidic Tumor Modeling and Biosensing

Bioprinted research models of urological malignancy

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