Drop of Egg Production in Chickens by Experimental Infection with an Avian Metapneumovirus Strain PLE8T1 Derived from Swollen Head Syndrome and the Application to Evaluate Vaccine

Sugiyama, Miki; Koimaru, Hiroyuki; Sato, Masahiro; Ono, Eriko; Nagata, Tadashi; Ito, Toshihiro

doi:10.1292/jvms.68.783

Cited by 22 publications

(16 citation statements)

References 16 publications

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“…In turkeys, it causes turkey rhinotracheitis and it is involved in a disease called swollen head syndrome in chickens. In young turkeys, signs such as ocular and nasal discharge, sneezing, coughing and swelling of the infraorbital sinuses may be observed (Jirjis et al, 2004;Sugiyama et al, 2006). The respiratory signs in aMPV-mono-infected chickens are normally less clear and milder than in turkeys (Lu et al, 1994;Banet-Noach et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Hartmann¹,

Sid²,

Rautenschlein³

2015

Avian Pathology

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Avian metapneumovirus (aMPV) is a pathogen with worldwide distribution, which can cause high economic losses in infected poultry. aMPV mainly causes infection of the upper respiratory tract in both chickens and turkeys, although turkeys seem to be more susceptible. Little is known about virus-host interactions at epithelial surfaces after aMPV infection. Tracheal organ cultures (TOC) are a suitable model to investigate virus-host interaction in the respiratory epithelium. Therefore, we investigated virus replication rates and lesion development in chicken and turkey TOC after infection with a virulent aMPV subtype A strain. Aspects of the innate immune response, such as interferon-α and inducible nitric oxide synthase mRNA expression, as well as virus-induced apoptosis were determined. The aMPV-replication rate was higher in turkey (TTOC) compared to chicken TOC (CTOC) (P < 0.05), providing circumstantial evidence that indeed turkeys may be more susceptible. The interferon-α response was down-regulated from 2 to 144 hours post infection in both species compared to virus-free controls (P < 0.05); this was more significant for CTOC than TTOC. Inducible nitric oxide synthase expression was significantly up-regulated in aMPV-A-infected TTOC and CTOC compared to virus-free controls (P < 0.05). However, the results suggest that NO may play a different role in aMPV pathogenesis between turkeys and chickens as indicated by differences in apoptosis rate and lesion development between species. Overall, our study reveals differences in innate immune response regulation and therefore may explain differences in aMPV -A replication rates between infected TTOC and CTOC, which subsequently lead to more severe clinical signs and a higher rate of secondary infections in turkeys.

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Section: Introductionmentioning

confidence: 99%

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Hartmann¹,

Sid²,

Rautenschlein³

2015

Avian Pathology

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“…aMPV causes turkey rhinotracheitis (TRT), an acute respiratory tract infection in turkeys of all ages. The virus is also associated with swollen head syndrome (SHS) in ______________________ broilers and broiler breeders (7) and egg production losses in layers (8). In recent years, outbreaks of respiratory disease have been increased in poultry farms in Iran.…”

Section: Introductionmentioning

confidence: 99%

The Role Of Avian Metapenumovirus In Respiratory Complex Disease Circulating In Broilers In Northern Iran

Seifi¹,

Boroomand²

2015

TJS

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Avian metapneumovirus (aMPV) is the etiologic agent of a respiratory disease of poultry. In a 6 months period from October 2012 to March 2013, broiler flocks with respiratory signs are examined in northern Iran. The purpose of the given research was to define presence of antibodies of the birds' avian metapneumovirus among broilers and detection of virus. Birds were not vaccinated against this virus.Total 525 blood samples and trachea/nasal turbinates swabs were obtained from 35 commercial broiler flocks and pooled (5 swabs/pool), then RT-PCR method was done on these 105 pooled samples. Presence of antibodies against the bird's metapneumovirus in each serum sample has been tested by method of immune-enzyme analysis. 10 (28.5%) flocks had positive antibodies. Of the 35 flocks, 8 were positive by RT-PCR with Nd/Nx primers (23%). This is the first report of APV infection in northern Iran. Vaccination programs should be adjusted to include the APV vaccine to aid in the control of this respiratory disease in the Iranian poultry industry.

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“…aMPV causes turkey rhinotracheitis, an acute respiratory tract infection in turkeys of all ages. The virus is also associated with swollen head syndrome (SHS) in broilers and broiler breeders (O'Brien, 1985;Wyeth et al, 1987;Pattison et al, 1989;Jones et al, 1991;Gough et al, 1994) and egg production losses in layers (Cook et al, 1996(Cook et al, , 2000Sugiyama et al, 2006). It has been reported that chicken aMPV isolates are antigenically closely related to turkey isolates (Cook et al, 1993a), but in previous investigations aMPV isolates from turkeys did not induce clinical signs in chickens (Gough et al, 1988;Buys et al, 1989b).…”

Section: Introductionmentioning

confidence: 99%

Reproducibility of swollen sinuses in broilers by experimental infection with avian metapneumovirus subtypes A and B of turkey origin and their comparative pathogenesis

et al. 2008

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Swollen head syndrome (SHS) associated with avian metapneumovirus (aMPV) subtype A or subtype B in broilers and broiler breeders has been reported worldwide. Data about pathogenesis of aMPV subtypes A and B in broilers are scarce. It has been difficult to reproduce swollen sinuses in chickens with aMPV under experimental conditions. In the field, SHS in broilers is suspected to be induced by combined infections with different respiratory pathogens. The objectives of the present study were to compare the pathogenesis of subtypes A and B aMPV in commercial broilers and to investigate the reproducibility of clinical disease. In two repeat experiments, commercial broilers free of aMPV maternal antibodies were inoculated with aMPV subtypes A and B of turkey origin. The clinical signs such as depression, coughing, nasal exudates, and frothy eyes appeared at 4 days post inoculation, followed by swelling of periorbital sinuses at 5 days post inoculation. Higher numbers of broilers showed clinical signs in subtype-B-inoculated compared with subtype-A-inoculated groups. Seroconversion to aMPV was detectable from 10 to 11 days post inoculation. The appearance of serum aMPV enzyme-linked immunosorbent assay antibodies and the clearance of the aMPV genome coincided. Subtype B aMPV showed a broader tissue distribution and longer persistence than subtype A. Histopathological changes were observed in the respiratory tract tissues of aMPV-inoculated broilers, and also in paraocular glands, such as the Harderian and lachrymal glands. Overall, our study shows that representative strains of both aMPV turkey isolates induced lesions in the respiratory tract, accompanied by swelling of infraorbital sinuses, indicating the role of aMPV as a primary pathogen for broilers.

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Drop of Egg Production in Chickens by Experimental Infection with an Avian Metapneumovirus Strain PLE8T1 Derived from Swollen Head Syndrome and the Application to Evaluate Vaccine

Cited by 22 publications

References 16 publications

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

The Role Of Avian Metapenumovirus In Respiratory Complex Disease Circulating In Broilers In Northern Iran

Reproducibility of swollen sinuses in broilers by experimental infection with avian metapneumovirus subtypes A and B of turkey origin and their comparative pathogenesis

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