Abstract:Previous studies on drop impact have mainly focused on homogeneous substrates while heterogeneous substrates have remained largely unexplored. A convenient preparation strategy of stiff heterogeneous substrates is presented in this work, and the drop impact on such stiffness-patterned substrates consisting of soft spirals surrounded by a rigid region is systematically investigated. The results show that the splash behavior of a drop on stiffness-patterned substrates exhibits distinct characteristics from that … Show more
“…2 Moreover, tilapia cultivated in Lake Kariba was also strongly affected by the outbreak of lactococcosis. 3,4 Golden pompano (Trachinotus ovatus), a kind of economically important marine fish species, is mainly distributed in the subtropical and tropical sea areas of Southeast Asian countries 5 and is an economically crucial cultured fish species in China with the production of 239,000 tons in 2021. 6 In recent years, the frequent disease occurrence of T. ovatus has caused serious economic losses due to overcrowding, extreme water temperatures, poor water quality, and nutrition in the fast development of the intensive aquaculture.…”
Lactococcus garvieae is one of the primary pathogens causing the severe disease to golden pompano (Trachinotus ovatus). Vaccination is the most effective measure of preventing the occurrence and spread of infectious diseases. However, no commercially available vaccine protects T. ovatus against L. garvieae infection. In the present study, the formalin-inactivated L. garvieae vaccines, consisting of bacterin alone (aqueous vaccine) and combined with oil adjuvant Montanide™ ISA 763 A VG (vaccine+763A), were prepared and administered to T. ovatus by intraperitoneal injection. The protective efficacy was evaluated by testing the relative percent survival (RPS), serum enzyme activity, and expression levels of immune-related genes in the tissues of T. ovatus after immunization. The results indicated that the relative percentage survival (RPS) values of aqueous vaccine and vaccine+763A were 80% and 90.8% at 8 weeks post-vaccination. The level of antibody titer, lysozyme activities, superoxide dismutase, and catalase in the two vaccinated fish groups increased significantly compared to that in the control group. Moreover, the expression levels of immune-related genes IgM, TNF-α, IL-8, MHCI-α, MHCII, CD4, CD8α, and IL-1β in all the tested tissues from T. ovatus were significantly enhanced after inoculation with aqueous vaccine and vaccine+763A. These findings demonstrated that the developed vaccine combined with oil adjuvant ISA 763 A VG improved immune responses and provided good protective efficacy against L. garvieae infection in the golden pompano culture.
“…2 Moreover, tilapia cultivated in Lake Kariba was also strongly affected by the outbreak of lactococcosis. 3,4 Golden pompano (Trachinotus ovatus), a kind of economically important marine fish species, is mainly distributed in the subtropical and tropical sea areas of Southeast Asian countries 5 and is an economically crucial cultured fish species in China with the production of 239,000 tons in 2021. 6 In recent years, the frequent disease occurrence of T. ovatus has caused serious economic losses due to overcrowding, extreme water temperatures, poor water quality, and nutrition in the fast development of the intensive aquaculture.…”
Lactococcus garvieae is one of the primary pathogens causing the severe disease to golden pompano (Trachinotus ovatus). Vaccination is the most effective measure of preventing the occurrence and spread of infectious diseases. However, no commercially available vaccine protects T. ovatus against L. garvieae infection. In the present study, the formalin-inactivated L. garvieae vaccines, consisting of bacterin alone (aqueous vaccine) and combined with oil adjuvant Montanide™ ISA 763 A VG (vaccine+763A), were prepared and administered to T. ovatus by intraperitoneal injection. The protective efficacy was evaluated by testing the relative percent survival (RPS), serum enzyme activity, and expression levels of immune-related genes in the tissues of T. ovatus after immunization. The results indicated that the relative percentage survival (RPS) values of aqueous vaccine and vaccine+763A were 80% and 90.8% at 8 weeks post-vaccination. The level of antibody titer, lysozyme activities, superoxide dismutase, and catalase in the two vaccinated fish groups increased significantly compared to that in the control group. Moreover, the expression levels of immune-related genes IgM, TNF-α, IL-8, MHCI-α, MHCII, CD4, CD8α, and IL-1β in all the tested tissues from T. ovatus were significantly enhanced after inoculation with aqueous vaccine and vaccine+763A. These findings demonstrated that the developed vaccine combined with oil adjuvant ISA 763 A VG improved immune responses and provided good protective efficacy against L. garvieae infection in the golden pompano culture.
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