Drimane-Type Sesquiterpenoids Derived from the Tropical Basidiomycetes Perenniporia centrali-africana and Cerrena sp. nov

Abstract

Five new drimane-type sesquiterpenoids were isolated from cultures of the tropical basidiomycetes, Perenniporia centrali-africana (originating from Kenya) and Cerrena sp. nov. (originating from Thailand). A new pereniporin A derivative (1), a new drimane-type sesquiterpene lactam (2), and the new 6,7-Dehydro-isodrimenediol (3) were isolated from P. centrali-africana. In parallel, the two new drimane-type sesquiterpene lactams 5 and 6 were isolated together with known isodrimenediol (4) from Cerrena sp. This is the first report of drimane-type sesquiterpene lactams from basidiomycetes. The structures were elucidated based on 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data, in combination with high-resolution electrospray mass spectrometric (HR-ESIMS) data. The compounds were devoid of significant antimicrobial and cytotoxic activities.

Neurite Outgrowth-Inducing Drimane-Type Sesquiterpenoids Isolated from Cultures of the Polypore Abundisporus violaceus MUCL 56355

“…All the isolated compounds were devoid of any significant activities in both the antimicrobial (12 strains) and cytotoxicity assays (two cell lines). Similar compounds have been reported to exhibit low to no activities in equivalent studies . Supported by the reported neurite outgrowth activity of other drimane sesquiterpenoids isolated from Cyathus africanus and C. stercoreus , , we decided to assess 1 – 6 in a neurite outgrowth assay in rat pheochromocytoma cells (PC-12), a well-established model system .…”

“…Similar compounds have been reported to exhibit low to no activities in equivalent studies. 27 Supported by the reported neurite outgrowth activity of other drimane sesquiterpenoids isolated from Cyathus africanus and C. stercoreus , 8 , 9 we decided to assess 1 – 6 in a neurite outgrowth assay in rat pheochromocytoma cells (PC-12), a well-established model system. 7 To measure the NGF-enhancing activity of compounds 1 – 6 , PC-12 cells were treated with 5 ng/mL of NGF in the presence or absence of the compound at a concentration of 5 μg/mL, respectively, and the mean neurite length was measured after 48 h of treatment ( Figure 9 ).…”

Neurite outgrowth-inducing Drimane-type Sesquiterpenoids Isolated from Cultures of the Polypore Abundisporus violaceus MUCL 56355

“…Similar compounds have been reported to exhibit low to no activities in equivalent studies. 27 Supported by the reported neurite outgrowth activity of other drimane sesquiterpenoids isolated from Cyathus africanus and C. stercoreus, 8,9 we decided to assess drimane-type sesquiterpenoid compounds (1-6) for neurite outgrowth assay in rat pheochromocytoma cells (PC-12), a wellestablished model system. 7 To measure the nerve growth factor (NGF) enhancing activity of compounds (1-6), PC-12 cells were treated with 5 ng/mL NGF in the presence or absence of the compound at a concentration of 5 µg/mL, respectively, and the mean neurite length was measured after 48 h of treatment (Figure 9).…”

ent-Clavilactone J and Its Quinone Derivative, Meroterpenoids from the Fungus Resupinatus sp.

“…Fungal natural products continue to offer great potential for new innovative drugs . Novel species of Basidiomycota, the second largest group of fungi after the Ascomycota, collected from Thailand have proven to be a rich source of new metabolites in our screening program for new secondary metabolites. − The present study deals with a Basidiomycota collected from northeastern Thailand and its secondary metabolites.…”