Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits

Abstract

This study was designed to evaluate the effects of drilling through the growth plate and using adipose-derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) to treat femoral head epiphyseal ischemic necrosis, which can be done in juvenile rabbits. Passagefour bromodeoxyuridine (BrdU)-labeled ADSCs were cultured, assayed with MTT to determine their viability and stained with alizarin red dye to determine their osteogenic ability. Two-month-old, healthy male rabbits (1.2 to 1.4 kg, n=45) underwent ischemic induction and were randomly divided into five groups (group A: animal model control; group B: drilling; group C: drilling & ADSCs; group D: drilling & BMP-2; and group E: drilling & ADSCs & BMP-2). Magnetic resonance imaging (MRI), X-ray imaging, hematoxylin and eosin staining and BrdU immunofluorescence detection were applied 4, 6 and 10 weeks after treatment. Approximately 90% of the ADSCs were labeled with BrdU and showed good viability and osteogenic ability. Similar results were observed in the rabbits in groups C and E at weeks 6 and 10. The animals of groups C and E demonstrated normal hip structure and improved femoral epiphyseal quotients and trabecular areas compared with those of the groups A and B (P<0.01). Group D demonstrated improved femoral epiphyseal quotients and trabecular areas compared with those of groups A and B (P<0.05). In summary, drilling through the growth plate combined with ADSC and BMP-2 treatments induced new bone formation and protected the femoral head epiphysis from collapsing in a juvenile rabbit model of femoral head epiphyseal ischemic necrosis.

Local transplantation of adipose-derived stem cells has a significant therapeutic effect in a mouse model of rheumatoid arthritis

“…It would be more appropriate to use human ADSCs from a clinical perspective. However, previous studies on ADSCs have demonstrated their effectiveness across several species [33][34][35] . Second, the severity of RA in our mouse model was evaluated on the basis of generalized clinical scoring.…”

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

“…Nevertheless, in profound skin wounds, the remaining skin stem cells cannot experience normal differentiation capacity and complete the anatomical structure and functional skin repair according to the preprogrammed pathways. Therefore, the healing progression may be disrupted, ultimately developing scar tissue devoid of the hair follicle and sweat glands [ 4 ]. This indicates that the process of wound healing is associated with interaction among cells, complex regulation of the extracellular matrix, and various paracrine elements [ 5 ].…”

Adipose Mesenchymal Stem Cells Combined with Platelet Rich Plasma Accelerate Diabetic Wound Healing by Modulating Notch Pathway

“…Nevertheless, in profound skin wounds, the remaining skin stem cells cannot experience normal differentiation capacity and complete the anatomical structure and functional skin repair according to the preprogrammed pathways. Therefore, the healing progression may be disrupted, ultimately developing scar tissue devoid of hair follicle and sweat glands (4). This indicates that the process of wound healing is associated with interaction among cells, complex regulation of the extracellular matrix, and various paracrine elements (5).…”