Dried urine spot and dried blood spot sample collection for rapid and sensitive monitoring of exposure to ricin and abrin by LC–MS/MS analysis of ricinine and l-abrine

Aviram, Lilach Yishai; Loewenthal, Dan; Hindi, Ariel; Gura, Sigalit; Weissberg, Avi; Dagan, S.

doi:10.1016/j.forc.2022.100438

Cited by 2 publications

(3 citation statements)

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“…Microsampling was also used in the high‐throughput identification of toxins and trace elements, where a highly sensitive LC‐MS/MS method was developed for the measurement of ricinine and L‐abrine in DBS, with respective detection limits of 50 and 100 pg/mL. The method's complete analytical time was less than 1 h, allowing for rapid preliminary confirmation of the presence of highly toxic proteins, ricin and abrin 155 . Although dried blood microsamples have increased stability for a variety of analytes, the authors showed that DBS performed poorly when quantifying trace elements such as aluminium, cadmium, cobalt and chromium, due to high signal contributions from blank filter paper and instability at room temperature.…”

Section: Applicationsmentioning

confidence: 99%

“…The method's complete analytical time was less than 1 h, allowing for rapid preliminary confirmation of the presence of highly toxic proteins, ricin and abrin. 155 Although dried blood microsamples have increased stability for a variety of analytes, the authors showed that DBS performed poorly when quantifying trace elements such as aluminium, cadmium, cobalt and chromium, due to high signal contributions from blank filter paper and instability at room temperature. This study demonstrated that microtubes were more suited than DBS for trace element quantification in human blood.…”

Section: Applicationsmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…Detection of abrin has been restricted to a few techniques, primarily relying on three distinct markers found in the seeds of Abrus precatorius: DNA, 7 the surrogate alkaloid L-abrine, [8][9][10] or the protein itself. 11 However, during further purification of abrin, the first two markers are lost in the preparation process.…”

Section: Introductionmentioning

confidence: 99%