Dried haematic microsamples and LC–MS/MS for the analysis of natural and synthetic cannabinoids

Protti, Michele; Rudge, James; Sberna, Angelo Eliseo; Gerra, Gilberto; Mercolini, Laura

doi:10.1016/j.jchromb.2016.12.038

Cited by 60 publications

(35 citation statements)

References 40 publications

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“…Extraction yields did not improve beyond the 500 μL limit, thus selected as the extraction volume. As regards VAMS devices, previous papers have decisively demonstrated their good sampling volume accuracy, both for blood‐based and for urine‐ and saliva‐based matrices. Sampling time was tested in the 2–10 s range.…”

Section: Resultsmentioning

confidence: 99%

“…The DMS strategy involves microvolumes of haematic matrix, to facilitate pretreatment, handling, storage and transport procedures. In addition to these features, VAMS are obtained using innovative samplers able to overcome certain specific disadvantages of dried matrices, such as haematocrit‐dependent volumetric bias and homogeneity issues . DMS and VAMS were applied for the monitoring of patients under therapy with SNP and the results were compared to those obtained with classic fluid blood sampling and plasma analysis.…”

Section: Introductionmentioning

confidence: 99%

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Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Protti

Vignali

Blanco

et al. 2018

J of Separation Science

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Asenapine is a recent drug approved in the European Union for the treatment of bipolar disorder. An original approach has been developed for asenapine analysis in patients treated with the drug, including miniaturized microsampling procedures, separation and quantitation of drug enantiomers. An original enantioselective method based on high-performance liquid chromatography with diode array detection was developed and applied to the determination of asenapine enantiomer levels in innovative haematic samples: four micromatrices have been tested, two based on dried matrix spots (dried blood spots and dried plasma spots) and two based on volumetric absorptive microsampling (from blood and plasma). Chiral separation was achieved on a cellulose-tris(3,5 dimethylphenylcarbamate) column, with a mobile phase containing bicarbonate buffer and acetonitrile. The method was validated with satisfactory results of linearity and precision on all matrices that showed also a significant performance in terms of stability, feasibility and reliability, when compared to fluid plasma sampling, handling and processing. Among micromatrices, both volumetric absorptive microsampling types were superior to dried matrix spots in terms of data reproducibility and correspondence with plasma levels. The bioanalytical approach proposed herein provides for the first time a chiral high-performance liquid chromatographic method for the determination of asenapine enantiomers, coupled to a very effective microsampling strategy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Protti

Vignali

Blanco

et al. 2018

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…The tip is capable of absorbing a fixed liquid sample volume (10, 20, or 30 µL), according to its size and independently of sample density or viscosity. After absorption, the specimen is left to dry, and is then ready for storage, shipping or immediate extraction, and analysis [13,14]. The use of fixed-volume dried microsamples offers several advantages over traditional, large-volume biosamples.…”

Section: Introductionmentioning

confidence: 99%

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

2020

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Reliable, feasible analytical methods are needed for forensic and anti-doping testing of cocaine and its most important metabolites, benzoylecgonine, ecgonine methyl ester, and cocaethylene (the active metabolite formed in the presence of ethanol). An innovative workflow is presented here, using minute amounts of dried blood or plasma obtained by volumetric absorptive microsampling (VAMS), followed by miniaturized pretreatment by dispersive pipette extraction (DPX) and LC-MS/MS analysis. After sampling 20 µL of blood or plasma with a VAMS device, the sample was dried, extracted, and loaded onto a DPX tip. The DPX pretreatment lasted less than one minute and after elution with methanol the sample was directly injected into the LC-MS/MS system. The chromatographic analysis was carried out on a C8 column, using a mobile phase containing aqueous formic acid and acetonitrile. Good extraction yield (> 85%), precision (relative standard deviation, RSD < 6.0%) and matrix effect (< 12%) values were obtained. Analyte stability was outstanding (recovery > 85% after 2 months at room temperature). The method was successfully applied to real blood and plasma VAMS, with results in very good agreement with those of fluid samples. The method seems suitable for the monitoring of concomitant cocaine and ethanol use by means of plasma or blood VAMS testing.

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“…As example, Mercolini et al [38] demonstrated the applicability of VAMS for the measurement of natural and synthetic cannabinoids including THC and its 2 major metabolites (THC-OH and THC-COOH) for forensic and sports drug testing purposes.…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

et al. 2020

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The aim of this work is to evaluate volumetric absorptive microsampling (VAMS) from capillary blood as an alternative strategy for therapeutic drug monitoring (TDM) in patients treated with the newly available GW-purified form of cannabidiol (Epidiolex®). A fast ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) coupled to an online sample preparation system analysis was carried out on a Thermo Scientific Ultimate 3000 LC system coupled to a TSQ Quantiva triple quadrupole for the quantification of cannabidiol (CBD) and, in addition, delta-9-tetrahydrocannabinol (Δ9-THC). After validation using European Medicine Agency (EMA) guidelines the method was applied to samples obtained by finger prick of five pediatric patients treated with Epidiolex® and the results were compared to those obtained from venous blood and plasma. The method is linear in the range of 1–800 µg/L for both CBD and THC with intra- and inter-day precisions ranging from 5% to 14% and accuracies from −13% to +14% starting from 30 µL of sample. Stability in VAMS is ensured for up to 4 weeks at 25 °C thus allowing simple delivery. There was no difference (p = 0.69) between concentrations of CBD measured from VAMS sampled from capillary or venous blood (range: 52.19–330.14 or 72.15–383.45 µg/L) and those obtained from plasma (range: 64.3–374.09 µg/L) The VAMS-LC-MS/MS method represents a valid alternative strategy for therapeutic drug monitoring of patients treated with Epidiolex®.

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Dried haematic microsamples and LC–MS/MS for the analysis of natural and synthetic cannabinoids

Cited by 60 publications

References 40 publications

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

Cannabidiol Determination on Peripheral Capillary Blood Using a Microsampling Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry with On-Line Sample Preparation

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