Dried blood spots analysis with mass spectrometry: Potentials and pitfalls in therapeutic drug monitoring

Antunes, Marina Venzon; Charão, Mariele Feiffer; Linden, Rafael

doi:10.1016/j.clinbiochem.2016.05.004

Cited by 114 publications

(61 citation statements)

References 71 publications

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“…Moreover, as DBS sampling is based on arterial capillary blood and some amount of interstitial fluid, the concentrations of circulating metabolites could potentially be different from venous blood. As these differences are dependent on the characteristics of particular drugs or diet xenobiotics, case‐to‐case evaluation is necessary to validate the method …”

Section: Introductionmentioning

confidence: 99%

Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red‐Fleshed Apple Snack

Yuste

Macià

Ludwig

et al. 2018

Molecular Nutrition Food Res

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Scope The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. Methods and results An analytical method based on blood sampling with DBS cards combined with LC–MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red‐fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl‐γ‐valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE. Conclusions Finger‐prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.

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Section: Introductionmentioning

confidence: 99%

Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red‐Fleshed Apple Snack

Yuste

Macià

Ludwig

et al. 2018

Molecular Nutrition Food Res

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“…Nowadays, the use of DBS sampling has become a common practice in several clinical applications, for the diagnosis of an inborn error of metabolism [2][3][4] and for therapeutic drug monitoring and clinical drug development [5,6], including toxicology [7].…”

Section: Introductionmentioning

confidence: 99%

Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

et al. 2017

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Volumetric absorptive microsampling (VAMS) is a novel approach that allows single-drop (10 μL) blood collection. Integration of VAMS with mass spectrometry (MS)-based untargeted metabolomics is an attractive solution for both human and animal studies. However, to boost the use of VAMS in metabolomics, key pre-analytical questions need to be addressed. Therefore, in this work, we integrated VAMS in a MS-based untargeted metabolomics workflow and investigated pre-analytical strategies such as sample extraction procedures and metabolome stability at different storage conditions. We first evaluated the best extraction procedure for the polar metabolome and found that the highest number and amount of metabolites were recovered upon extraction with acetonitrile/water (70:30). In contrast, basic conditions (pH 9) resulted in divergent metabolite profiles mainly resulting from the extraction of intracellular metabolites originating from red blood cells. In addition, the prolonged storage of blood samples at room temperature caused significant changes in metabolome composition, but once the VAMS devices were stored at − 80 °C, the metabolome remained stable for up to 6 months. The time used for drying the sample did also affect the metabolome. In fact, some metabolites were rapidly degraded or accumulated in the sample during the first 48 h at room temperature, indicating that a longer drying step will significantly change the concentration in the sample. KeywordsMetabolomics; Volumetric absorptive microsampling; Mass spectrometry ✉ Giuseppe Paglia beppepaglia@gmail.com. Compliance with ethical standardsThis study was performed in accordance with the ethical standards. The local ethics committee (Comitato etico del comprensorio sanitario di Bolzano) approved the study, and all participants provided written informed consent.

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“…For assessing the severity and thus the need of antidote treatment, the paracetamol concentration should be measured in a blood sample taken four hours after ingestion when absorption and distribution are almost completed. 4 Pitfalls concerning TDM in dried blood spots (DBS) are reviewed by Antunes et al 5 with special focus on influence of the hematocrit on the plasma concentrations. They discussed besides sensitivity, validation, and standardization issues, the influence of the blood sample hematocrit on translating DBS concentrations to reference plasma levels.…”

Section: Samplingmentioning

confidence: 99%

Pitfalls in drug testing by hyphenated low‐ and high‐resolution mass spectrometry

Maurer

2020

Drug Testing and Analysis

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This paper reviews various pitfalls observed during developing, validation, application, and interpretation of drug testing approaches using GC-MS and low-and high-resolution LC-MS. They include sampling and storage of body samples, sample adulteration and contamination, analyte stability, sample preparation without or with cleavage of conjugates, extraction, derivatization, internal standardization, false negative and positive results by GC-MS or LC-MS screening and/or confirmation procedures including artifact formation, ion suppression or enhancement by electrospray ionization, and finally pitfalls in data interpretation. Conclusions and prospects close the Tutorial. K E Y W O R D S

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Dried blood spots analysis with mass spectrometry: Potentials and pitfalls in therapeutic drug monitoring

Cited by 114 publications

References 71 publications

Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red‐Fleshed Apple Snack

Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red‐Fleshed Apple Snack

Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow

Pitfalls in drug testing by hyphenated low‐ and high‐resolution mass spectrometry

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