Dried aerobic heterotrophic bacteria from treatment of food and beverage effluents: Screening of correlations between operation parameters and microbial protein quality

Muys, Maarten; Papini, Gustavo; Spiller, Marc; Sakarika, Myrsini; Schwaiger, Barbara; Lesueur, Céline; Vermeir, Pieter; Vlaeminck, Siegfried E.

doi:10.1016/j.biortech.2020.123242

Cited by 21 publications

(12 citation statements)

References 38 publications

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“…Higher protein contents of 49.9, 49.5 and 48.7% as dry biomass were observed in reactors IF 4-5 , F 1-2 , and F 4-5 , respectively (Figure S2), regardless of the presence or absence of an initial inoculum. Similar protein percentages were observed in aerobic heterotrophic biomass produced from different food processing wastewaters (Muys et al, 2020). However, they were lower than reported values for other types of SCP biomass.…”

Section: Wastewaters With a Lower Scod:stkn (C:n) And Higher Stkn Pro...supporting

confidence: 82%

“…The same amino acids were also dominant in the existent microbial community (measured as dry cell mass) in soybean-processing wastewater (Figure S5). SCP produced from industrial wastewaters was reported to be suitable for replacing, at least partially, conventional fish meal (Hülsen et al, 2018b; Muys et al, 2020). When SCP produced from purple bacteria was used as shrimp feed, the animals exhibited higher tolerance of Vibrio pathogens and resistance to ammonia stress compared to a control group fed on conventional fish meal (Alloul et al, 2021b).…”

Section: Resultsmentioning

confidence: 99%

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Microbial community-based production of single cell protein from soybean-processing wastewater of variable chemical composition

Vethathirri

Santillan

Thi

et al. 2022

Preprint

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The use of food-processing wastewaters to produce microbial biomass-derived single cell protein (SCP) is a sustainable way to meet the global food demand. Yet, despite the potential benefits of lower costs and greater resource recovery compared to pure cultures, bioconversion processes relying on microbial community-based approaches to SCP production have received scarce attention. Here, we evaluated SCP production from soybean-processing wastewaters under controlled reactor conditions using the existent microbial communities in these wastewaters. Six sequencing batch reactors of 4.5-L working volume were operated at 30 ℃ for 34 d in cycles consisting of 3-h anaerobic and 9-h aerobic phases. Four reactors received no microbial inoculum and the remaining two were amended with a 1.5 L of mixed culture from a prior microbial community-based SCP production. Microbial characterization was done via 16S rRNA gene metabarcoding. Influent wastewater batches had variable chemical characteristics but a similar microbial composition. Reactors produced more SCP when fed with wastewaters of higher soluble total Kjeldahl nitrogen (sTKN) content and a lower carbon-to-nitrogen ratio (sCOD:sTKN). The biomass protein yield ranged from 0.24 to 3.13 g protein/g sTKN, with a maximum protein content of 50%. An average of 92% of sCOD and 73% of sTN removal was achieved. Distinct microbial communities were enriched in all six bioreactors after 34 d, where the prevailing genera included Azospirillum, Rhodobacter, Lactococcus, Novosphingobium, and Acidipropionibacterium. In contrast, the microbial community of influent wastewaters was dominated by Lactococcus and Weissella. We showed that constituents in soybean wastewater can be converted to SCP through microbial community-based growth processes and demonstrated the effect of variable influent wastewater composition on SCP production.

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Section: Wastewaters With a Lower Scod:stkn (C:n) And Higher Stkn Pro...supporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Microbial community-based production of single cell protein from soybean-processing wastewater of variable chemical composition

Vethathirri

Santillan

Thi

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…767/2009 prohibits the use of waste obtained from feces and urine as feed material. 4 Muys et al 5 performed a screening of 19 wastewater installations in the food and beverage industry and observed that 6 companies already separated process water and sanitary wastewater. The study of Muys et al 5 also showed that brewery and dairy wastewater are particularly interesting for protein production as heavy metals in the activated sludge were below legal limits for feed ingredients.…”

Section: Introductionmentioning

confidence: 99%

Operational Strategies to Selectively Produce Purple Bacteria for Microbial Protein in Raceway Reactors

Alloul

Cerruti

Adamczyk

et al. 2021

Environ. Sci. Technol.

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Purple non-sulfur bacteria (PNSB) show potential for microbial protein production on wastewater as animal feed. They offer good selectivity (i.e., low microbial diversity and high abundance of one species) when grown anaerobically in the light. However, the cost of closed anaerobic photobioreactors is prohibitive for protein production. Although open raceway reactors are cheaper, their feasibility to selectively grow PNSB is thus far unexplored. This study developed operational strategies to boost PNSB abundance in the biomass of a raceway reactor fed with volatile fatty acids. For a flask reactor run at a 2 day sludge retention time (SRT), matching the chemical oxygen demand (COD) loading rate to the removal rate in the light period prevented substrate availability during the dark period and increased the PNSB abundance from 50–67 to 88–94%. A raceway reactor run at a 2 day SRT showed an increased PNSB abundance from 14 to 56% when oxygen supply was reduced (no stirring at night). The best performance was achieved at the highest surface-to-volume ratio (10 m2 m–3 increased light availability) showing productivities up to 0.2 g protein L–1 day–1 and a PNSB abundance of 78%. This study pioneered in PNSB-based microbial protein production in raceway reactors, yielding high selectivity while avoiding the combined availability of oxygen, COD, and darkness.

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“…The composition of wastewater can vary depending on the source (Muys et al, 2020). These differences create an imbalance in nutrient availability, which can cause a shift in the metabolism and biomass composition (e.g.…”

Section: Influence Of the Chemical Oxygen Demand To Nitrogen Ratiomentioning

confidence: 99%

Aggregation of purple bacteria in an upflow photobioreactor to facilitate solid/liquid separation: Impact of organic loading rate, hydraulic retention time and water composition

Blansaer

Alloul

Verstraete

et al. 2022

Bioresource Technology

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Dried aerobic heterotrophic bacteria from treatment of food and beverage effluents: Screening of correlations between operation parameters and microbial protein quality

Cited by 21 publications

References 38 publications

Microbial community-based production of single cell protein from soybean-processing wastewater of variable chemical composition

Microbial community-based production of single cell protein from soybean-processing wastewater of variable chemical composition

Operational Strategies to Selectively Produce Purple Bacteria for Microbial Protein in Raceway Reactors

Aggregation of purple bacteria in an upflow photobioreactor to facilitate solid/liquid separation: Impact of organic loading rate, hydraulic retention time and water composition

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