DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2

Ni, Ting; Huang, Ting; Gu, Sheng-Lan; Wang, Jing; Liu, Yao; Sun, Xiao; Wang, Yudong

doi:10.7150/jca.40055

Cited by 12 publications

(9 citation statements)

References 45 publications

(57 reference statements)

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“…In endometrial cancer (EC), GluR2 (GRIA2) expression was upregulated, and the GluR2 antagonist effectively suppressed the invasion, migration, and proliferation of tumor cells. Both in vitro and invivo, EC cells showed its tropism toward DRG neurons and neuron ber which implied that glutamate receptor signal and neuron-tumor interaction play a signi cant role in EC growth (25). Moreover, in the central nervous system, previous studies demonstrated that the generation of synapse and glutamate receptor signals were linked to the progression of high-grade glioma and breast tumor patients, which corroborates with our results of LGG patients (12,(26)(27)(28).…”

Section: Discussionsupporting

confidence: 91%

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Lin

Yang

Hou

et al. 2020

Preprint

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BackgroundSynapse and synapse associated proteins (SAPs) play critical roles in various neurodegeneration diseases and brain tumors. However, in lower-grade gliomas (LGG), SAPs have not been explored systematically. Herein, we are going to explore SAPs expression profile and its clinicopathological significance in LGG which can offer new insights to glioma therapy. MethodIn this study, we used five sources including, Venkatesh, Shen, Colón, Jüttner R, Gene Ontology (GO) project to integrate a list of SAPs that covered 231 proteins with synaptogenesis activity and post synapse formation. The LGG RNA-seq data were downloaded from gene expression omnibus (GEO) database, The Cancer Genome Atlas (TCGA), and Chinese Glioma Genome Atlas (CGGA). The differentially expressed SAPs were filtered out and constructed PPI to search for key modules and SAPs. Then, using Kaplan–Meier survival analysis, least absolute shrinkage and selection operator (LASSO), and multicox regression analysis, the prognostic significance of these key SAPs was evaluated. CGGA database, Human Protein Altas (HPA) and quantitative real-time PCR were used to verify our findings. ResultData from function enrichment analysis revealed functions of differentially expressed SAPs in synapse organization and glutamatergic receptor pathway in LGGs. Survival analysis revealed four SAPs, GRIK2, GABRD, GRID2, ARC that were correlate with the prognosis of LGG patients and used to construct the prognostic models. Among them, the expression of GABRD was lower in glioma tissue than normal brain tissue, but higher in seizure LGG patients than non-seizure patients. The four-SAPs signature was revealed as an independent prognostic factor in gliomas. ConclusionOur study presented a novel strategy to assess the prognostic risks of LGGs, based on the expression of SAPs. Also, we revealed that several SAPs upregulated in patients with seizures, indicating that they are linked to the pathogenesis of seizures in glioma patients.

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Section: Discussionsupporting

confidence: 91%

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Lin

Yang

Hou

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In endometrial cancer (EC), GluR2 (GRIA2) expression was upregulated, and the GluR2 antagonist effectively suppressed the invasion, migration, and proliferation of tumor cells. Both in vitro and in vivo, EC cells showed its tropism toward DRG neurons and neuron ber which implied that glutamate receptor signal and neuron-tumor interaction play a signi cant role in EC growth (25). Moreover, in the central nervous system, previous studies demonstrated that the generation of synapse and glutamate receptor signals were linked to the progression of high-grade glioma and breast tumor patients, which corroborates with our results of LGG patients (12,(26)(27)(28).…”

Section: Discussionsupporting

confidence: 90%

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Lin

Yang

Hou

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Synapse and synapse associated proteins (SAPs) play critical roles in various neurodegeneration diseases and brain tumors. However, in lower-grade gliomas (LGG), SAPs have not been explored systematically. Herein, we are going to explore SAPs expression profile and its clinicopathological significance in LGG which can offer new insights to glioma therapy. Method: In this study, we used five sources including, Venkatesh, Shen, Colón, Jüttner R, Gene Ontology (GO) project to integrate a list of SAPs that covered 231 proteins with synaptogenesis activity and post synapse formation. The LGG RNA-seq data were downloaded from gene expression omnibus (GEO) database, The Cancer Genome Atlas (TCGA), and Chinese Glioma Genome Atlas (CGGA). The differentially expressed SAPs were filtered out and constructed PPI to search for key modules and SAPs. Then, using Kaplan–Meier survival analysis, least absolute shrinkage and selection operator (LASSO), and multicox regression analysis, the prognostic significance of these key SAPs was evaluated. CGGA database, Human Protein Altas (HPA) and quantitative real-time PCR were used to verify our findings. Result: Data from function enrichment analysis revealed functions of differentially expressed SAPs in synapse organization and glutamatergic receptor pathway in LGGs. Survival analysis revealed four SAPs, GRIK2, GABRD, GRID2, ARC that were correlate with the prognosis of LGG patients and used to construct the prognostic models. Among them, the expression of GABRD was lower in glioma tissue than normal brain tissue, but higher in seizure LGG patients than non-seizure patients. The four-SAPs signature was revealed as an independent prognostic factor in gliomas.Conclusion: Our study presented a novel strategy to assess the prognostic risks of LGGs, based on the expression of SAPs. Also, we revealed that several SAPs upregulated in patients with seizures, indicating that they are linked to the pathogenesis of seizures in glioma patients.

show abstract

“…In endometrial cancer (EC), GluR2 (GRIA2) expression was up-regulated, and the GluR2 antagonist effectively suppressed the invasion, migration and proliferation of tumor cells. Both in vitro and in vivo , EC cells showed its tropism toward DRG neurons and neuron fiber which implied that glutamate receptor signal and neuron–tumor interaction play a significant role in EC growth [ 41 ]. Moreover, in the central nervous system, previous studies demonstrated that the generation of synapse and glutamate receptor signals were linked to the progression of high-grade glioma and breast tumor patients, which corroborates with our results of LGG patients [ 16–18 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Validation of the functions and prognostic values of synapse-associated proteins in lower-grade glioma

et al. 2021

View full text Add to dashboard Cite

Synapse and synapse associated proteins (SAPs) play critical roles in various neurodegeneration diseases and brain tumors. However, in lower-grade gliomas (LGG), SAPs have not been explored systematically. Herein, we are going to explore SAPs expression profile and its clinicopathological significance in LGG which can offer new insights to glioma therapy. In this study, we integrate a list of SAPs that covered 231 proteins with synaptogenesis activity and post synapse formation. The LGG RNA-seq data were downloaded from GEO, TCGA and CGGA database. The prognosis associated SAPs in key modules of PPI (protein-protein interaction networks) was regarded as hub SAPs. Western blot, quantitative reverse transcription PCR (qRT-PCR) and immunochemistry results from HPA database were used to verify the expression of hub SAPs. There were 68 upregulated SAPs and 44 downregulated SAPs in LGG tissue compared with normal brain tissue. Data from function enrichment analysis revealed functions of differentially expressed SAPs in synapse organization and glutamatergic receptor pathway in LGGs. Survival analysis revealed that four SAPs, GRIK2, GABRD, GRID2 and ARC were correlate with the prognosis of LGG patients. Interestingly, we found that GABRD were upregulated in LGG patients with seizures, indicating that SAPs may link to the pathogenesis of seizures in glioma patients. The four-SAPs signature was revealed as an independent prognostic factor in gliomas. Our study presented a novel strategy to assess the prognostic risks of LGGs, based on the expression of SAPs.

show abstract

DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2

Cited by 12 publications

References 45 publications

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Validation of the Functions and Prognostic Values of Synapse Associated Proteins in Lower-Grade Glioma

Validation of the functions and prognostic values of synapse-associated proteins in lower-grade glioma

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