Drep-2 is a novel synaptic protein important for learning and memory

Andlauer, Till F. M.; Scholz-Kornehl, Sabrina; Tian, Rui; Kirchner, Marieluise; Babikir, Husam; Depner, Harald; Loll, Bernhard; Quentin, Christine; Gupta, Varun; Holt, Matthew; Dipt, Shubham; Cressy, Michael; Wahl, Markus C.; Fiala, André; Selbach, Matthias; Schwärzel, Martin; Sigrist, Stephan J.

doi:10.7554/elife.03895

Cited by 46 publications

(47 citation statements)

References 93 publications

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“…However, on a proteomic dataset with log‐transformed intensities, such as for instance those reported in , fudge2() advised s 0 ≈0.02. This is significantly smaller than other values reported in proteomics literature (see for instance where s 0 ranges between 0.4 and 4).…”

contrasting

confidence: 62%

Uses and misuses of the fudge factor in quantitative discovery proteomics

et al. 2016

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Selecting proteins with significant differential abundance is the cornerstone of many relative quantitative proteomics experiments. To do so, a trade-off between p-value thresholding and fold-change thresholding can be performed because of a specific parameter, named fudge factor, and classically noted s 0 . We have observed that this fudge factor is routinely turned away from its original (and statistically valid) use, leading to important distortion in the distribution of p-values, jeopardizing the protein differential analysis, as well as the subsequent biological conclusion. In this article, we provide a comprehensive viewpoint on this issue, as well as some guidelines to circumvent it.

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contrasting

confidence: 62%

Uses and misuses of the fudge factor in quantitative discovery proteomics

et al. 2016

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“…Immunohistochemistry was performed according to our standard protocol (Andlauer et al, 2014). Conventional confocal and STED images were acquired with a TCS SP8 and TCS SP8 gSTED 3× microscope (Leica Microsystems, Wetzlar, Germany), respectively.…”

Section: Methodsmentioning

confidence: 99%

Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses

et al. 2018

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High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, and frequency tuning of synaptic vesicle release are also highly diversified across brain synapses. These features critically depend on the nanometer-scale coupling distance between voltage-gated Ca channels (VGCCs) and the synaptic vesicle release machinery. Combining light super resolution microscopy with in vivo electrophysiology, we show here that two orthogonal scaffold proteins (ELKS family Bruchpilot, BRP, and Syd-1) cluster-specific (M)Unc13 release factor isoforms either close (BRP/Unc13A) or further away (Syd-1/Unc13B) from VGCCs across synapses of the Drosophila olfactory system, resulting in different synapse-characteristic forms of short-term plasticity. Moreover, BRP/Unc13A versus Syd-1/Unc13B ratios were different between synapse types. Thus, variation in tightly versus loosely coupled scaffold protein/(M)Unc13 modules can tune synapse-type-specific release features, and "nanoscopic molecular fingerprints" might identify synapses with specific temporal features.

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“…Surprisingly, Drep2 was found to be a synaptic protein highly expressed in the CNS, without any evident role in apoptosis (Andlauer et al 2014). Drep2 is enriched at the PSD of mushroom body input synapses, colocalizing with the ACh receptor subunit D⍺7 and directly apposing Brp.…”

Section: Regulation Of Synaptic Organizationmentioning

confidence: 99%

Transmission, Development, and Plasticity of Synapses

Harris¹,

2015

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Chemical synapses are sites of contact and information transfer between a neuron and its partner cell. Each synapse is a specialized junction, where the presynaptic cell assembles machinery for the release of neurotransmitter, and the postsynaptic cell assembles components to receive and integrate this signal. Synapses also exhibit plasticity, during which synaptic function and/or structure are modified in response to activity. With a robust panel of genetic, imaging, and electrophysiology approaches, and strong evolutionary conservation of molecular components, Drosophila has emerged as an essential model system for investigating the mechanisms underlying synaptic assembly, function, and plasticity. We will discuss techniques for studying synapses in Drosophila, with a focus on the larval neuromuscular junction (NMJ), a well-established model glutamatergic synapse. Vesicle fusion, which underlies synaptic release of neurotransmitters, has been well characterized at this synapse. In addition, studies of synaptic assembly and organization of active zones and postsynaptic densities have revealed pathways that coordinate those events across the synaptic cleft. We will also review modes of synaptic growth and plasticity at the fly NMJ, and discuss how pre-and postsynaptic cells communicate to regulate plasticity in response to activity. KEYWORDS FlyBook; Drosophila; synapse; neurotransmitter release; synaptic plasticity; active zone; synaptic vesicle TABLE OF CONTENTS Abstract 345Introduction 345

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Drep-2 is a novel synaptic protein important for learning and memory

Cited by 46 publications

References 93 publications

Uses and misuses of the fudge factor in quantitative discovery proteomics

Uses and misuses of the fudge factor in quantitative discovery proteomics

Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses

Transmission, Development, and Plasticity of Synapses

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