Drawing Sticky Adeno‐Associated Viruses on Surfaces for Spatially Patterned Gene Expression

Kim, Eun‐Mi; Song, In Taek; Lee, Slgirim; Kim, Jung Suk; Lee, Haeshin; Jang, Jae Won

doi:10.1002/anie.201201495

Cited by 33 publications

(15 citation statements)

References 21 publications

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“…Importantly, as visualized in Figure A, the exposure of pDA particles on the external 3D‐PCL_pD sponge surfaces resulted in the robust immobilization of soluble factors (Figure A,B), followed by their retarded releases (Figure C,D). The catechol groups exposed on the surface can induce the adhesion chemistry that primarily involves covalent bond formation via reactions, such as Michael addition or Schiff base formation, with the organic molecules presenting either on numerous soluble factors (e.g., proteins, virus) or on tissue surfaces . Consequently, the reactions of catechol groups with amines on the BSA‐FITC or AAV vectors resulted in approximately 1.5‐to‐5‐fold increases in their immobilizations on the 3D‐PCL_pD surfaces, compared to those on the 2D‐PCL and 3D‐PCL matrices (Figure A,B).…”

Section: Resultsmentioning

confidence: 99%

“…A novel AAV vector (i.e., AAV r3.45), which demonstrated enhanced gene delivery performances in various clinically valuable cell types, was used to evaluate the resulting fibrous constructs as gene delivery vehicles. Recombinant AAV r3.45 vectors carrying cDNA coding for luciferase (Luc) driven by a cytomegalovirus (CMV) promoter were produced by a transient transfection, as previously described .…”

Section: Methodsmentioning

confidence: 99%

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Polydopamine Inter‐Fiber Networks: New Strategy for Producing Rigid, Sticky, 3D Fluffy Electrospun Fibrous Polycaprolactone Sponges

Choi

Lee

Kim

et al. 2016

Macromolecular Bioscience

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Designing versatile 3D interfaces that can precisely represent a biological environment is a prerequisite for the creation of artificial tissue structures. To this end, electrospun fibrous sponges, precisely mimicking an extracellular matrix and providing highly porous interfaces, have capabilities that can function as versatile physical cues to regenerate various tissues. However, their intrinsic features, such as sheet-like, thin, and weak structures, limit the design of a number of uses in tissue engineering applications. Herein, a highly facile methodology capable of fabricating rigid, sticky, spatially expanded fluffy electrospun fibrous sponges is proposed. A bio-inspired adhesive material, poly(dopamine) (pDA), is employed as a key mediator to provide rigidity and stickiness to the 3D poly(ε-caprolactone) (PCL) fibrous sponges, which are fabricated using a coaxial electrospinning with polystyrene followed by a selective leaching process. The iron ion induced oxidation of dopamine into pDA networks interwoven with PCL fibers results in significant increases in the rigidity of 3D fibrous sponges. Furthermore, the exposure of catecholamine groups on the fiber surfaces promotes the stable attachment of the sponges on wet organ surfaces and triggers the robust immobilization of biomolecules (e.g., proteins and gene vectors), demonstrating their potential for 3D scaffolds as well as drug delivery vehicles. Because fibrous structures are ubiquitous in the human body, these rigid, sticky, 3D fibrous sponges are good candidates for powerful biomaterial systems that functionally mimic a variety of tissue structures.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Polydopamine Inter‐Fiber Networks: New Strategy for Producing Rigid, Sticky, 3D Fluffy Electrospun Fibrous Polycaprolactone Sponges

Choi

Lee

Kim

et al. 2016

Macromolecular Bioscience

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“…4C ). Combined with the ability to functionalize microparticles of different core materials (e.g., magnetite or magnetic polystyrene), the utility of MCMs for localized transfection may further enable applications such as facile spatial patterning of gene expression in vitro , an area in which previous approaches have required specialized viral vectors 37 , surface coatings 38 , or microfluidic devices 39 .…”

Section: Discussionmentioning

confidence: 99%

Functionalization of microparticles with mineral coatings enhances non-viral transfection of primary human cells

Khalil

Xie

Fontana

et al. 2017

Sci Rep

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Gene delivery to primary human cells is a technology of critical interest to both life science research and therapeutic applications. However, poor efficiencies in gene transfer and undesirable safety profiles remain key limitations in advancing this technology. Here, we describe a materials-based approach whereby application of a bioresorbable mineral coating improves microparticle-based transfection of plasmid DNA lipoplexes in several primary human cell types. In the presence of these mineral-coated microparticles (MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in cytotoxicity. We identified mechanisms by which MCMs improve transfection, as well as coating compositions that improve transfection in three-dimensional cell constructs. The approach afforded efficient transfection in primary human fibroblasts as well as mesenchymal and embryonic stem cells for both two- and three-dimensional transfection strategies. This MCM-based transfection is an advancement in gene delivery technology, as it represents a non-viral approach that enables highly efficient, localized transfection and allows for transfection of three-dimensional cell constructs.

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“…Additionally, modifications of surface properties, as achieved by producing self-assembled monolayers or by engaging organosilane chemistry, have been employed to regulate interactions with cells [9]. The manipulation of surface properties, resulting in variations in cellular attachment and cellular patterning, has been attempted using various lithographic techniques, including writing-based lithography [10][11][12] or surface graft polymerization approaches [13]. Additionally, the topographical changes of three-dimensional cell-culture structures [14], well-defined structural patterns [15], and surface rigidity [16] directly influenced cell adhesion on substrates and survivals as well.…”

Section: Introductionmentioning

confidence: 99%

The Promotion of Human Neural Stem Cells Adhesion Using Bioinspired Poly(norepinephrine) Nanoscale Coating

Park

Shin

Kim

et al. 2014

Journal of Nanomaterials

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The establishment of versatile biomaterial interfaces that can facilitate cellular adhesion is crucial for elucidating the cellular processes that occur on biomaterial surfaces. Furthermore, biomaterial interfaces can provide physical or chemical cues that are capable of stimulating cellular behaviors by regulating intracellular signaling cascades. Herein, a method of creating a biomimetic functional biointerface was introduced to enhance human neural stem cell (hNSC) adhesion. The hNSC-compatible biointerface was prepared by the oxidative polymerization of the neurotransmitter norepinephrine, which generates a nanoscale organic thin layer, termed poly(norepinephrine) (pNE). Due to its adhesive property, pNE resulted in an adherent layer on various substrates, and pNE-coated biointerfaces provided a highly favorable microenvironment for hNSCs, with no observed cytotoxicity. Only a 2-hour incubation of hNSCs was required to firmly attach the stem cells, regardless of the type of substrate. Importantly, the adhesive properties of pNE interfaces led to micropatterns of cellular attachment, thereby demonstrating the ability of the interface to organize the stem cells. This highly facile surface-modification method using a biomimetic pNE thin layer can be applied to a number of suitable materials that were previously not compatible with hNSC technology.

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Drawing Sticky Adeno‐Associated Viruses on Surfaces for Spatially Patterned Gene Expression

Cited by 33 publications

References 21 publications

Polydopamine Inter‐Fiber Networks: New Strategy for Producing Rigid, Sticky, 3D Fluffy Electrospun Fibrous Polycaprolactone Sponges

Polydopamine Inter‐Fiber Networks: New Strategy for Producing Rigid, Sticky, 3D Fluffy Electrospun Fibrous Polycaprolactone Sponges

Functionalization of microparticles with mineral coatings enhances non-viral transfection of primary human cells

The Promotion of Human Neural Stem Cells Adhesion Using Bioinspired Poly(norepinephrine) Nanoscale Coating

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