'Drawing' a Molecular Portrait of CIN and Cervical Cancer: a Review of Genome-Wide Molecular Profiling Data

Kurmyshkina, Olga; Ковчур, П. И.; Volkova, Tatyana O.

doi:10.7314/apjcp.2015.16.11.4477

Cited by 6 publications

(4 citation statements)

References 90 publications

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“…A previous study revealed that cervical lesion is an infectious disease caused by HPV [1]. Persistent high-risk HPV infection is a major risk factor to induce cervical lesions and promote its progression [6]. However, the vast majority of HPV infections and CIN I, half of CIN II, and 30% of CIN III cases, can be spontaneously reversed [7], which ultimately does not cause cervical cancer [8].…”

Section: Discussionmentioning

confidence: 99%

Difference in vaginal microecology, local immunity and HPV infection among childbearing-age women with different degrees of cervical lesions in Inner Mongolia

Zheng

Song

et al. 2019

BMC Women's Health

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Background This study aims to investigate the difference in vaginal microecology, local immunity and HPV infection among childbearing-age women with different degrees of cervical lesions. Methods A total of 432 patients were included in this study. Among these patients, 136 patients had LSIL, 263 patients had HSIL and 33 patients had CSCC. These patients were assigned as the research groups. In addition, 100 healthy females were enrolled and assigned as the control group. Results The microbiological indexes of vaginal secretions were evaluated. Furthermore, the concentrations of SIgA, IgG, IL-2 and IL-10 in vaginal lavage fluid, as well as the presence of HPV, mycoplasma and Chlamydia in cervical secretions, were detected. The results is that: (1) Differences in evaluation indexes of vaginal microecology among all research groups and the control group were statistically significant ( P < 0.0001). As the degree of cervical lesions increased, the number of Lactobacillus decreased, and there was an increase in prevalence of bacterial imbalance, and the diversity, density and normal proportion of bacteria was reduced. Furthermore, the incidence of HPV, trichomonads, clue cell and Chlamydia infection increased. Moreover, the positive rate of H 2 O 2 decreased, while the positive rates of SNa and GADP increased. (2) Differences in the ratio of IL-2 and IL-10 in the female genital tract among all research groups and the control group were statistically significant ( P < 0.0001). Conclusions As the degree of cervical lesions increased, IL-2 decreased, IL-10 increased and IL-2/IL-10 decreased, while SIgA and IgG were elevated. The reduction of dominant Lactobacillus in the vagina, impairment of H 2 O 2 function, flora ratio imbalance, pathogen infections, reduction in IL-2/IL-10 ratio, and changes in SIgA and IgG levels could all be potential factors that influenced the pathogenicity of HPV infection and the occurrence and development of cervical lesions.

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Section: Discussionmentioning

confidence: 99%

Difference in vaginal microecology, local immunity and HPV infection among childbearing-age women with different degrees of cervical lesions in Inner Mongolia

Zheng

Song

et al. 2019

BMC Women's Health

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“…Uprising research on cancer has defined the genetic and epigenetic basis as fundamental but complex phenomenon that interacts during the development and progression of malignant cells into invasive tumors. 43 Though numerous population based studies have been performed but due to poorly understood biology, multiple risk factors, 44 molecular abnormalities, 45 as well as genetic diversity necessitated continuous investigation of novel targets in cervical carcinogenesis. This study, therefore, aimed at determining the contribution of genetic and epigenetic aberrations among novel and critical tumor suppressor component (genes), such as PARK-2 linking it not only with cervical carcinogenesis but their association with pathological markers which could be a promising approach in combating the fatal disease in future.…”

Section: Discussionmentioning

confidence: 99%

Genetic and epigenetic alterations affecting PARK-2 expression in cervical neoplasm among North Indian patients

et al. 2017

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The recent investigation on PARK-2, a putative tumor suppressor gene, has found that it has been altered in multiple human malignancies. However, the clinical impact of PARK-2 alteration in uterine cervix carcinoma has not yet been studied. Therefore, we aimed to examine mutations, promoter hypermethylation, and protein expression of PARK-2 among the North Indian patients and their association with clinical parameters to evaluate the implication of PARK-2 in the genesis of cervical cancer. A total of 168 patient samples were processed for mutational analysis by single-strand conformation polymorphism, sequencing, and further in silico analysis of the identified mutations. Promoter hypermethylation by methylation-specific polymerase chain reaction and expression of PARK-2 were performed using immunohistochemistry. Statistical correlation between molecular findings and the clinicopathological parameters was taken to figure out the meaningful outcome. As per our findings, 3.5% (6/168) tumors showed novel missense mutations in exon 11 of PARK-2. In silico analysis showed high structural deviations manifested by mutations, A398D and Y391N, in both mutant proteins as compared to wild type. Promoter hypermethylation was observed in total of 29% of (48/168) tumor samples. Furthermore, 46.43% tumors (78/168) exhibited loss of PARK-2 expression in cervical carcinoma. The loss of expression of PARK-2 when correlated with clinical parameters resulted in significant association with tumor stage (p = 0.002) and with histological grade (p = 0.025). However, only clinical stage remained significant after Bonferroni correction (p < 0.007). A trend was observed between PARK-2 promoter hypermethylation and its protein expression. Our study provided sufficient information and insight for investigation of PARK-2 and highlighted its role as a tumor suppressor gene in cervical cancer in North Indian population.

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“…Each molecular profile (genome, transcriptome, proteome, etc.) in the dynamics of the development of the disease progression gradually accumulates multiple aberrations and thus contributes to the development of the tumor [81]. Despite the large number of publications dedicated to various aspects of cervical carcinogenesis, pathologists still play an important role in the development of diagnostic approaches and targeted therapies aimed at tumors characterized by a certain phenotype and the presence of biomarkers that allow the response to targeted therapy to be predicted, enabling the monitoring of response to treatment [92].…”

Section: Vaginal Microbiome Inflammation and Immune Homeostasismentioning

confidence: 99%

Cervical Carcinoma: Oncobiology and Biomarkers

Volkova

Pashov

Omelchuk

2021

IJMS

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Cervical cancer is one of the most common types of carcinomas causing morbidity and mortality in women in all countries of the world. At the moment, the oncology, oncobiology, and oncomorphology of cervical cancer are characterized by the accumulation of new information; various molecular biological, genetic, and immunohistochemical methods of investigation of the mechanisms of cervical carcinogenesis are tested and applied; targeted antitumour drugs and diagnostic, prognostic, and predictive biomarkers are being searched for. Many issues of the etiopathogenesis of cervical cancer have not been sufficiently studied, and the role of many biomarkers characterizing various stages of cervical carcinogenesis remains unclear. Therefore, the target of this review is to systematize and understand several problems in the pathogenesis of cervical cancer and to evaluate the significance and role of biomarkers in cervical carcinogenesis.

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'Drawing' a Molecular Portrait of CIN and Cervical Cancer: a Review of Genome-Wide Molecular Profiling Data

Cited by 6 publications

References 90 publications

Difference in vaginal microecology, local immunity and HPV infection among childbearing-age women with different degrees of cervical lesions in Inner Mongolia

Difference in vaginal microecology, local immunity and HPV infection among childbearing-age women with different degrees of cervical lesions in Inner Mongolia

Genetic and epigenetic alterations affecting PARK-2 expression in cervical neoplasm among North Indian patients

Cervical Carcinoma: Oncobiology and Biomarkers

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