30Meiotic drivers are parasitic loci that force their own transmission into greater than half of the 31 offspring of a heterozygote. Many drivers have been identified, but their molecular mechanisms 32 are largely unknown. The wtf4 gene is a meiotic driver in Schizosaccharomyces pombe that 33 uses a poison-antidote mechanism. Here, we show that the Wtf4 proteins can function outside 34 of gametogenesis and in a distantly related species, Saccharomyces cerevisiae. The Wtf4 poison 35 protein forms dispersed, toxic aggregates. The similar Wtf4 antidote protein also forms aggregates 36 but is sequestered within or near vacuoles and is mostly benign. The Wtf4 antidote can co-37 assemble with the Wtf4 poison and promote its trafficking to vacuoles. We show that neutralization 38 of the Wtf4 poison requires both co-assembly with the Wtf4 antidote and aggregate sequestration, as 39 mutations that disrupt either of these processes results in cell death. This work reveals that wtf 40 parasites can exploit protein aggregate management pathways to selectively destroy gametes.