Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

Abstract

Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

Tooth injuries: Knowledge of parents of public school students from the city of Alfenas, Minas Gerais, Brazil

“…Of the parents who answered that they would immerse it in liquid (22.3%), most of them chose saline solution (17.8%) or water (16.4%) over milk (12.2%), which ranked third. Milk is considered the ideal storage medium for avulsed teeth as it has been shown to maintain cells for up to 6 hours . These findings identified the gaps in caregiver's knowledge that should be addressed.…”

“…Although immediate replantation is considered the optimal treatment to preserve the cells of the periodontal ligament, delayed replantation is the most common approach, because the adult at the place of the trauma is not always able to do the replantation. Thus, the success of the replantation depends on the integrity of the cells of periodontal ligament that is strongly influenced by the storage medium, extra‐alveolar period, and root preservation conditions . When asked about the possibility of replanting an avulsed tooth, 65.5% of the parents believe this procedure is possible.…”

“…In addition, they were asked about the most suitable medium to store the avulsed tooth and the parents reported that they would wrap the tooth in gauze or cotton. However, it is well known that replantation outcomes are improved by wet storage of the avulsed tooth to preserve the periodontal ligament cells . Of the parents who answered that they would immerse it in liquid (22.3%), most of them chose saline solution (17.8%) or water (16.4%) over milk (12.2%), which ranked third.…”

Periodontal healing by periodontal ligament fiber with or without cells: A preclinical study of the decellularized periodontal ligament in a tooth replantation model

“…1 A previous in vitro study showed the multipotential of these PDL cells to differentiate into cells with various phenotypes, such as bone, adipose tissue, cartilage/ligament, and cementum. 3,4 Clinical periodontal healing is also affected by factors other than PDL cells. 3,4 Clinical periodontal healing is also affected by factors other than PDL cells.…”

Conhecimento de estudantes de 9 a 12 anos do CEF GAN, Asa Norte-DF, sobre trauma dental, após atividade educativa

“…Esse fato decorre da não orientação do paciente diante do trauma, e pesquisas mostram que, para se manter um metabolismo celular satisfatório, as substâncias devem ser repostas de 60 a 120 minutos após o acidente 7 . Após 5 minutos o processo de reabsorção pós-reimplante começa a se mostra mais significante 8 sendo que, quanto mais rápido o elemento voltar ao seu alvéolo, maiores são as possibilidades de sucesso 9 , por isso a necessidade de se transmitir a informação sobre esse assunto, nos ambientes escolares, onde se encontra grande parte do público alvo. Segundo Subramaniam et al 10 , a avulsão dentária representa 0.5 à 16% das lesões traumáticas na dentição permanente, ocorrendo com mais frequência em pacientes de 7 a 9 anos de idade.…”