Draft Whole-Genome Sequences of Seven Isolates of Klebsiella pneumoniae from New Zealand Sea Lions

Pinpimai, Komkiew; Roe, Wendi D.; Biggs, Patrick J.; Dittmer, Keren E.

doi:10.1128/mra.01270-18

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…In human cases in Asia, sequence type 86 (ST86) and 65 (ST65) are the most prevalent among the serotype K2 group (29), which is similar to what we found in this study. The isolates from California sea lion, and marmoset in our study are ST65, while others (isolates from New Zealand sea lions and the isolate from marmoset from Brazil) are ST86 (15,30). These can be implied that there is a genetic variation of hypervirulent K. pneumoniae in animals, suggesting that there is no specific genetic type for each species of animals.…”

Section: Discussionmentioning

confidence: 55%

Genetic characterization of hypervirulent Klebsiella pneumoniae responsible for acute death in captive marmosets

et al. 2022

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Klebsiella pneumoniae is a Gram-negative bacterium implicated as the causative pathogen in several medical health issues with different strains causing different pathologies including pneumonia, bloodstream infections, meningitis and infections from wounds or surgery. In this study, four captive African marmosets housed in Thailand were found dead. Necropsy and histology revealed congestion of hearts, kidneys and adrenal glands. Twenty-four bacterial isolates were obtained from these four animals with all isolates yielding identical phenotypes indicative of K. pneumoniae based on classical identification schema. All the isolates show the susceptibility to amikacin, cephalexin, doxycycline, gentamicin, and enrofloxacin with intermediate susceptibility to amoxycillin/clavulanic acid. One isolate (20P167W) was chosen for genome analysis and determined to belong to sequence type 65 (ST65). The genome of 20P167W possessed multiple virulence genes including mrk gene cluster and iro and iuc gene cluster (salmochelin and aerobactin, respectively) as well as multiple antibiotic resistance genes including blaSHV−67, blaSHV−11, oqxA, oqxB, and fosA genes resembling those found in human isolates; this isolate has a close genetic relationship with isolates from humans in Ireland, but not from Thailand and California sea lions. Phylogenetic studies using SNP show that there was no relation between genetic and geographic distributions of all known strains typing ST65, suggesting that ST65 strains may spread worldwide through multiple international transmission events rather than by local expansions in humans and/or animals. We also predict that K. pneumoniae ST65 has an ability to acquire genetic mobile element from other bacteria, which would allow Klebsiella to become an even greater public health concern.

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Section: Discussionmentioning

confidence: 55%

Genetic characterization of hypervirulent Klebsiella pneumoniae responsible for acute death in captive marmosets

et al. 2022

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Global spread and evolutionary convergence of multidrug-resistant and hypervirulent Klebsiella pneumoniae high-risk clones

Arcari

Carattoli

2022

Pathogens and Global Health

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Hypervirulent Klebsiella pneumoniae

2019

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SUMMARY Hypervirulent K. pneumoniae (hvKp) is an evolving pathotype that is more virulent than classical K. pneumoniae (cKp). hvKp usually infects individuals from the community, who are often healthy. Infections are more common in the Asian Pacific Rim but are occurring globally. hvKp infection frequently presents at multiple sites or subsequently metastatically spreads, often requiring source control. hvKp has an increased ability to cause central nervous system infection and endophthalmitis, which require rapid recognition and site-specific treatment. The genetic factors that confer hvKp’s hypervirulent phenotype are present on a large virulence plasmid and perhaps integrative conjugal elements. Increased capsule production and aerobactin production are established hvKp-specific virulence factors. Similar to cKp, hvKp strains are becoming increasingly resistant to antimicrobials via acquisition of mobile elements carrying resistance determinants, and new hvKp strains emerge when extensively drug-resistant cKp strains acquire hvKp-specific virulence determinants, resulting in nosocomial infection. Presently, clinical laboratories are unable to differentiate cKp from hvKp, but recently, several biomarkers and quantitative siderophore production have been shown to accurately predict hvKp strains, which could lead to the development of a diagnostic test for use by clinical laboratories for optimal patient care and for use in epidemiologic surveillance and research studies.

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Draft Whole-Genome Sequences of Seven Isolates of Klebsiella pneumoniae from New Zealand Sea Lions

Abstract: Klebsiella pneumoniae is a Gram-negative bacterium that may cause infection in a broad range of hosts. We report here the genome sequences of seven K. pneumoniae isolates from New Zealand sea lions.

Cited by 4 publications

References 15 publications

Genetic characterization of hypervirulent Klebsiella pneumoniae responsible for acute death in captive marmosets

Genetic characterization of hypervirulent Klebsiella pneumoniae responsible for acute death in captive marmosets

Global spread and evolutionary convergence of multidrug-resistant and hypervirulent Klebsiella pneumoniae high-risk clones

Hypervirulent Klebsiella pneumoniae

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