Draft Whole-Genome Sequence of Psychrotrophic
            <i>Arthrobacter</i>
            sp. Strain 7749, Isolated from Antarctic Marine Sediments with Applications in Enantioselective Alcohol Oxidation

Sastre, Diego E.; Santos, Leidaiany P.; Kagohara, Edna; Andrade, Leandro H.

doi:10.1128/genomea.01197-17

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Similar to the genomic size of strain AQ5-06, a 4.12 Mb draft genome sequence for Arthrobacter sp. strain 7749 isolated from marine sediment samples from the Antarctic Peninsula was reported by Sastre et al [35]. Figure 1 displays the number of genes in each subsystem and the subsystem coverage.…”

Section: Detection Of Phenol Degradative Genes Via Wgsmentioning

confidence: 74%

“…Members of the genus Arthrobacter are known as high-GC-content Actinobacteria, consistent with the findings here and on the previously reported Arthrobacter spp. [32][33][34][35]. According to Kim et al [36], members of the genus Arthrobacter typically have a DNA GC content of 59 to 66%, in agreement with the genome of strains AQ5-05 and AQ5-06.…”

Section: Detection Of Phenol Degradative Genes Via Wgsmentioning

confidence: 98%

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Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.

et al. 2022

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Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments.

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Section: Detection Of Phenol Degradative Genes Via Wgsmentioning

confidence: 74%

Section: Detection Of Phenol Degradative Genes Via Wgsmentioning

confidence: 98%

Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.

et al. 2022

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“…Arthrobacter sp. 7749 can oxidize phenylethanol derivatives (Sastre, Santos, Kagohara, & Andrade, 2017). The protein sequences of LS16, YC‐RL1, and 7749 were downloaded from NCBI and were subjected to pairwise alignment using local blast‐2.4.0 + software ( E < 10 −5 ).…”

Section: Resultsmentioning

confidence: 99%

Combined genomic and transcriptomic analysis of the dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Liu

Qiu

et al. 2020

Biotech & Bioengineering

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Dibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of a river of Hangzhou, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP compared with glucose revealed specific DBP metabolic pathways in the ZJUTW strain. The degrading gene clusters localize separately on a circular chromosome and a plasmid pQL1. Genes related to the initial steps of DBP degradation from DBP to phthalic acid (PA), the pehA gene, and pht gene cluster, are located on the plasmid pQL1. However, the pca gene cluster related to the transforming of intermediate protocatechuic acid (PCA) to acetyl-CoA, is located on the chromosome. After comparative analysis with the reported gene clusters, we found that there were a series of homologous genes in pht and pca gene clusters that contribute to the efficient degradation of DBP by ZJUTW. In addition, transcriptomic analysis suggested a synergistic effect between pht and pca clusters, which also favor ZJUTW allowing it to efficiently degrade DBP. Combined genomic and transcriptomic analyses revealed a complete DBP metabolic pathway in Arthrobacter sp. ZJUTW that is different from that of other reported Arthrobacter strains. After necessary modification based on its metabolic characteristics, Arthrobacter sp. ZJUTW or its derivatives might represent promising candidates for the bioremediation of DBP pollution.

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“…Arthrobacter sp. 7749 can oxidize phenylethanol derivatives (Sastre, Santos, Kagohara, & Andrade, 2017). The protein sequences of LS16, YC-RL1, and 7749 were obtained from NCBI and were subjected to pairwise alignment using local blast-2.4.0+ software (E-value < 10 -5 ).…”

Section: Comparative Genomics Analysis Between Zjutw and Other 25 Arthrobacter Strainsmentioning

confidence: 99%

Combined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Liu¹,

Li²,

Qiu³

et al.

Authorea

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Dibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of river of Hangzhou city, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP and with glucose revealed specific DBP metabolic pathways in the ZJUTW strain. The degrading gene clusters distribute separately on a circular chromosome and a plasmid pQL1. Genes related to the initial steps of DBP degradation from DBP to phthalic acid (PA), the pehA gene and pht gene cluster, are located on the plasmid pQL1. While pca gene cluster related to the transforming of protocatechuic acid (PCA) to acetyl-CoA, is located on the chromosome. After homologous alignment analysis with the reported gene clusters, we found that there were a series of double copies of homologous genes in pht and pca gene clusters that contribute to the efficient degradation of DBP by ZJUTW. In addition, transcriptomic analysis showed a synergistic effect between pht and pca clusters, which also favor ZJUTW allowing it to efficiently degrade DBP. Combined genomic and transcriptomic analyses affords the complete DBP metabolic pathway in Arthrobacter sp. ZJUTW that is different from that of reported other Arthrobacter strains. After necessary modification based on its metabolic characteristics, Arthrobacter sp. ZJUTW or its mutants might represent promising candidates for use in the bioremediation of DBP pollution.

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Draft Whole-Genome Sequence of Psychrotrophic Arthrobacter sp. Strain 7749, Isolated from Antarctic Marine Sediments with Applications in Enantioselective Alcohol Oxidation

Cited by 5 publications

References 13 publications

Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.

Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.

Combined genomic and transcriptomic analysis of the dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Combined genomic and transcriptomic analysis of Dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

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