Draft Nuclear Genome Sequence of the Halophilic and Beta-Carotene-Accumulating Green Alga
            <i>Dunaliella salina</i>
            Strain CCAP19/18

Polle, Juergen; Barry, Kerrie; Cushman, John C.; Schmutz, Jeremy; Tran, Duc; Hathwaik, Leyla T.; Yim, Won Cheol; Jenkins, Jerry; McKie‐Krisberg, Zaid; Prochnik, Simon; Lindquist, Erika; Dockter, Rhyan B.; Adam, Catherine; Molina, Henrik; Bunkenborg, Jakob; Jin, EonSeon; Buchheim, Mark A.; Magnuson, Jon

doi:10.1128/genomea.01105-17

Cited by 89 publications

(67 citation statements)

References 16 publications

(10 reference statements)

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“…Consistently, a variety of genes involved in inositol‐phosphate‐metabolism are present in the genome of D. salina strain CCAP19/18 (Polle et al. ). Therefore, elucidating the underlying mechanism of MI's roles in microalgae is meaningful for both the usage of MI in microalgal cultivations for aquacultural feeds, nutraceuticals, and algae‐based biodiesel, as well as understanding its roles in carbon metabolism and signaling during algal development.…”

mentioning

confidence: 77%

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹

Wang

et al. 2020

Journal of Phycology

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Dunaliella salina is well known for its ability to accumulate large amounts of β‐carotene. Myo‐inositol (MI) enhances the biomass production of D. salina, but the underlying mechanisms were unclear. The present study showed that the concentration of exogenous MI decreased gradually and reached a constant level at the 4th day of cultivation. MI enhanced the contents of total colored carotenoids and the activity of photosystem II. Metabolic profiles were significantly changed after the addition of exogenous MI, as revealed by multivariate statistical analysis. The metabolites could be categorized into four groups based on the relative levels in different samples. Exogenous MI increased the levels of most detected sugars, amino acids, and total saturated and unsaturated fatty acids. Based on the physiological and metabolic analyses, a hypothetical growth‐promoting model that MI promotes the growth of D. salina TG by increasing the levels of key metabolites and possibly enhancing photosynthesis, was proposed. This study provides valuable information for understanding the growth‐promoting mechanisms of MI in D. salina from the metabolic perspective.

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confidence: 77%

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹

Wang

et al. 2020

Journal of Phycology

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“…Through the analysis of the D. salina transcriptome and genome data (https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Dsalina) [45], one mitogen-activated protein kinase kinase (MAPKK, also known as MEK) gene was found to be highly similar. At the same time, we also used RACE technology to amplify DsMEK1 (Table S1 No.…”

Section: Gene Cloning Dna Sequencing Bioinformatic Analysis and Phmentioning

confidence: 99%

“…The identified motifs were annotated based on CDD (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). According to the cloned ORF of DsMEK1 and the genome sequence of DsMEK1 in the D. salina genome [45], the exon sequence was obtained, and the primers were designed (Table S2) sativa MAPKK sequences were obtained from the NCBI database. D. salina DsMEK2 was obtained from the phytozome database (http://www.phytozome.net/) [46].…”

Section: Gene Cloning Dna Sequencing Bioinformatic Analysis and Phmentioning

confidence: 99%

Two splice variants of the DsMEK1 mitogen-activated protein kinase kinase (MAPKK) are involved in salt stress regulation in Dunaliella salina in different ways

Zhong

Cao

Zhang

et al. 2020

Preprint

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Background: Dunaliella salina can produce glycerol under salt stress, and this production can quickly adapt to changes in external salt concentration. Notably, glycerol is an ideal energy source. In recent years, it has been reported that the mitogen-activated protein kinase cascade pathway plays an important role in regulating salt stress, and in Dunaliella tertiolecta DtMAPK can regulate glycerol synthesis under salt stress. Therefore, it is highly important to study the relationship between the MAPK cascade pathway and salt stress in D. salina and modify it to increase the prodution of glycerol. Results: In our study, we identified and analysed the alternative splicing of DsMEK1 (DsMEK1-X1, DsMEK1-X2) from the unicellular green alga D. salina. DsMEK1-X1 and DsMEK1-X2 were both localized in the cytoplasm. qRT-PCR assays showed that DsMEK1-X2 was induced by salt stress. Overexpression of DsMEK1-X2 revealed a higher increase rate of glycerol production compared to the control and DsMEK1-X1-oe under salt stress. Under salt stress, the expression of DsGPDH2/3/5/6 increased in DsMEK1-X2-oe strains compared to the control. This finding indicated that DsMEK1-X2 was involved in the regulation of DsGPDH expression and glycerol overexpression under salt stress. Overexpression of DsMEK1-X1 increased the proline content and reduced the MDA content under salt stress, and DsMEK1-X1 was able to regulate oxidative stress; thus, we hypothesized that DsMEK1-X1 could reduce oxidative damage under salt stress. Yeast two-hybrid analysis showed that DsMEK1-X2 could interact with DsMAPKKK1/2/3/9/10/17 and DsMAPK1; however, DsMEK1-X1 interacted with neither upstream MAPKKK nor downstream MAPK. DsMEK1-X2-oe transgenic lines increased the expression of DsMAPKKK1/3/10/17 and DsMAPK1, and DsMEK1-X2-RNAi lines decreased the expression of DsMAPKKK2/10/17. DsMEK1-X1-oe transgenic lines did not exhibit increased gene expression, except for DsMAPKKK9. Conclusion: Our findings demonstrate that DsMEK1-X1 and DsMEK1-X2 can respond to salt stress by two different pathways. The DsMEK1-X1 response to salt stress reduces oxidative damage; however, the DsMAPKKK1/2/3/9/10/17- DsMEK1-X2-DsMAPK1 cascade is involved in the regulation of DsGPDH expression and thus glycerol synthesis under salt stress.

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“…This membrane consists of a glycocalyx-like coating and, therefore, Dunaliella is susceptible to osmolar changes in the environment and exhibit flexible cell morphology (Polle et al 2017).…”

Section: The Diversity Of Extracellular Matricesmentioning

confidence: 99%

Green microalgae biomolecule separations and recovery

Dixon

Wilken

2018

Bioresour. Bioprocess.

105

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Microalgae biomass has garnered significant attention as a renewable energy feedstock and alternative to petroleumbased fuels. The diverse metabolism of green microalgae species additionally provides opportunities for recovery of products for feed, food, nutraceutical, cosmetic, and biopharmaceutical industries. Recently, the concept of using microalgae as part of a biorefinery model has been adopted in place of refinery methods focused on recovering one target product. This has led to producers exploring co-production of high value and high volume products in an effort to improve process economics. With numerous potential products and applications, the biomass source or specific strain must be carefully selected to accumulate extractable levels of the target molecule(s). It is additionally imperative to understand the morphology and metabolism of the selected strain to cost-effectively manage all stages of commercial production. This review will focus specifically on microalgae in the division of Chlorophyta, or green algae and their extracellular matrices (ECM), potential for commercial products, and finally describe a holistic approach for biomolecule extraction and recovery. Additionally, cell disruption and fractionation methods for recovery of biomolecules for commercial products are highlighted along with an alternative method, aqueous enzymatic processing for multiple biomolecule extraction and recovery from green microalgae. An emphasis is placed on connecting the morphological characteristics of microalgae ECM or organelle membranes to implications on separation and purification technologies.

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Draft Nuclear Genome Sequence of the Halophilic and Beta-Carotene-Accumulating Green Alga Dunaliella salina Strain CCAP19/18

Cited by 89 publications

References 16 publications

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹

Two splice variants of the DsMEK1 mitogen-activated protein kinase kinase (MAPKK) are involved in salt stress regulation in Dunaliella salina in different ways

Green microalgae biomolecule separations and recovery

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Draft Nuclear Genome Sequence of the Halophilic and Beta-Carotene-Accumulating Green Alga Dunaliella salina Strain CCAP19/18

Cited by 89 publications

References 16 publications

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol1

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol1

Two splice variants of the DsMEK1 mitogen-activated protein kinase kinase (MAPKK) are involved in salt stress regulation in Dunaliella salina in different ways

Green microalgae biomolecule separations and recovery

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Product

Resources

About

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹

Physiological and Metabolic Responses of a Novel Dunaliella salina Strain to Myo‐inositol¹