Draft Nuclear Genome Sequence of the Liquid Hydrocarbon–Accumulating Green Microalga
            <i>Botryococcus braunii</i>
            Race B (Showa)

Browne, Daniel R.; Jenkins, Jerry; Schmutz, Jeremy; Shu, Shengqiang; Barry, Kerrie; Chiniquy, Jennifer; Sharma, Aditi; Niehaus, Thomas D.; Weiss, Taylor L.; Koppisch, Andrew T.; Fox, David T.; Dhungana, Suraj; Okada, Shigeru; Chappell, Joe; Devarenne, Timothy P.

doi:10.1128/genomea.00215-17

Cited by 22 publications

(17 citation statements)

References 9 publications

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“…Also, two squalene epoxidase (BbSQE-I and -II) enzymes converting squalene into membrane sterols were identified [45]. Data of the B. braunii race B nuclear genome will allow the search for possible regulatory routes of this singular metabolism [46].…”

Section: Lycopadiene Biosynthetic Pathway (A) Reduction Of Ggpp To Pmentioning

confidence: 99%

The Colonial Microalgae Botryococcus braunii as Biorefinery

Lozoya‐Gloria¹,

Cruz²,

Ozawa-Uyeda³

2020

Microalgae - From Physiology to Application

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The growing shortage of fossil fuels caused an increase in the demand for alternative and renewable fuels. Biofuels, like bioethanol and biodiesel, have received more attention as a sustainable replacement of fossil fuels. However, these have a poor oxidative stability, little energy content by volume, and many oxygenated compounds, which may cause corrosion and damage to the engines. Therefore, they are used as a mixture with standard fuels. Some species of microalgae are candidates to produce oils as triglycerides (TGA) to produce biodiesel by transesterification; however, the problem will remain. The colonial microalgae Botryococcus braunii produces and accumulates a high amount of long-chain nonoxygenated hydrocarbons, similar to those obtained from the fractionated distillation of crude petroleum. This is one of the few organisms reported to have a direct contribution in the formation of the oil reserves currently in use. Additionally, B. braunii produces pigments and long-chain carbohydrates that have interesting properties for various industries. There are still problems to be solved in order to consider it as economically viable and profitable, but important progress is being made. Therefore, this microalga is very attractive for the synthesis of hydrocarbons and other value-added compounds, making it an interesting biorefinery organism.

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Section: Lycopadiene Biosynthetic Pathway (A) Reduction Of Ggpp To Pmentioning

confidence: 99%

The Colonial Microalgae Botryococcus braunii as Biorefinery

Lozoya‐Gloria¹,

Cruz²,

Ozawa-Uyeda³

2020

Microalgae - From Physiology to Application

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“…The gene duplication of triterpene biosynthesis enzymes in B. braunii appears to be unique among green algae whose genome has been investigated so far. Genome sequencing and contig assembling in B. braunii race B has been obtained (Browne et al 2017). The genome size of this alga is 166.2 Mb (Weiss et al 2010) and is much larger than that of a green alga Medakamo hakoo (Kuroiwa et al 2015(Kuroiwa et al , 2016.…”

Section: Biosyntheses Of Isoprenoids and Triterpenesmentioning

confidence: 99%

Algal Genes Encoding Enzymes for Photosynthesis and Hydrocarbon Biosynthesis as Candidates for Genetic Engineering

et al. 2018

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In order to increase the biomass of photosynthetic organisms, upregulation of net CO 2 fixation in daytime appears to be a promising approach. This can be attained by improving the turnover of chloroplast enzymes such as ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) or fructose bisphosphate phosphatase (FBPase). In a previous report, the genes for the large and small subunits of cyanobacterial Rubisco were coexpressed in tobacco with either with cyanobacterial chaperone for Rubisco or the carboxysomal CO 2 concentrating mechanism (CCM) gene. This resulted in the generation of faster carboxylation of Rubisco and a similar gene expression approach may be adopted into algal hosts in the future. Expression of the cyanobacterial FBPase gene with protein targeting to the chloroplast in Euglena gracilis was reported to increase the wax ester content effectively. Though stable transformation of the oil producing alga Botryococcus braunii race B, is yet to be achieved, genes for key regulatory enzymes in 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, such as 1-deoxy-Dxylulose 5-phosphate synthase (DXS) or 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), might be possible targets for genetic modification in this alga. Challenging problems in the transformation of the microalgae, including C. reinhardtii, or those of the macroalgae are discussed herein for the design of transformation aiming at higher yield of biofuel production.

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“…Chlamydomonas reinhardtii, Botryococcus braunii and Synechococcus elongatus genomes have been completely sequenced, and there are several genetic transformation and analytic tools that convert them into model organisms for hydrogen production [82][83][84][85]. In this direction, some work has been done to seek improved photosynthetic efficiency by truncation of photoreceptor antennas.…”

Section: Hydrogenmentioning

confidence: 99%

“…This route leads to a reduction in the activity of photosystem II, decreasing the production of oxygen and thus favoring the activity of the hydrogenase. Parallel to this, the effect of light selfattenuation also decreases [84][85][86]. To carry out the process on a large scale, some authors have proposed to limit the entry of sulphate to the chloroplast by creating mutant strains with altered activity of the sulphate permease at the chloroplast envelope.…”

Section: Hydrogenmentioning

confidence: 99%

Microalgae biorefineries: applications and emerging technologies

Giraldo

Romo-Buchelly

Arbeláez-Pérez

et al. 2018

DYNA

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Microalgae transform CO2 into a broad portfolio of biomolecules, and thus should be considered as a valuable biotechnological platform. Despite several research programs and global efforts to establish a sustainable microalgae-based industry, most applications have not transcended academic boundaries. This limitation raises from the high transformation costs when target products are biofuels or fertilizers. Microalgae biorefinery emerges as an alternative to improve economic competitiveness, as Under such a model scope, the process inputs come from industrial waste, while biomass exploitation prioritizes the highest value molecules followed by extraction of less valuable compounds. This review describes a wide range of microalgae exploitation schemes focused on new uses of its constituents, while discussing emerging technologies designed to improve production and efficiencies as well.Keywords: microalgae; biorefinery; biofuels; fertilizers; active biomolecules. Biorefinerías de microalgas: aplicaciones actuales y nuevas tecnologías en desarrolloResumen Las microalgas transforman el CO2 en un amplio portafolio de biomoléculas, por lo cual, son consideradas una valiosa plataforma biotecnológica. A pesar de múltiples programas de investigación y esfuerzos globales para establecer una industria sostenible basada en microalgas, la mayoría de las aplicaciones potenciales no han trascendido las fronteras académicas. Esta limitación se debe a los altos costos en la transformación del producto principalmente cuando se obtiene compuestos económicos como biocombustibles y fertilizantes. La biorefinería de microalgas surge como alternativa para incrementar la competitividad económica. En este modelo, los insumos del proceso provienen de residuos industriales, mientras que la explotación de la biomasa inicia con las moléculas de alto valor y finaliza con los compuestos menos valiosos. En esta revisión se describe un amplio abanico de esquemas de explotación de microalgas enfocado en nuevos usos de sus constituyentes. Además, se exploran las tecnologías emergentes destinadas a aprovechar esta biomasa de una manera más versátil y eficiente.

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Draft Nuclear Genome Sequence of the Liquid Hydrocarbon–Accumulating Green Microalga Botryococcus braunii Race B (Showa)

Cited by 22 publications

References 9 publications

The Colonial Microalgae Botryococcus braunii as Biorefinery

The Colonial Microalgae Botryococcus braunii as Biorefinery

Algal Genes Encoding Enzymes for Photosynthesis and Hydrocarbon Biosynthesis as Candidates for Genetic Engineering

Microalgae biorefineries: applications and emerging technologies

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