Draft mitochondrial genomes of <i>Hirudo medicinalis</i> and <i>Hirudo verbana</i> (Annelida, Hirudinea).

Nikitina, Anastasia S.; Babenko, Vladislav V.; Akopian, T. A.; Shirokov, Dmitriy A.; Manuvera, Valentin A.; Kurdyumov, Alexey S.; Kostryukova, Elena S.; Лазарев, В. Н.

doi:10.1080/23802359.2016.1157774

Cited by 9 publications

(7 citation statements)

References 7 publications

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“…Ozobranchus jantseanus and Zeylanicobdella arugamensis were rooted as outgroups ( Figure 1 ). The phylogenetic tree indicated that H. crenata r epresents a basal branch separated from the other Hirudinea species, and the relationship between those Hirudinea leeches was similar to previous researches (Nikitina et al 2016 ; Liu et al 2017 ).…”

supporting

confidence: 87%

Characterization of the complete mitogenome of a land leech, Haemadipsa crenata Ngamprasertwong (Arhynchobdellida: Haemadipsidae)

Wang

Tong

et al. 2021

Mitochondrial DNA Part B

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Land leeches of genus Haemadipsa (Family Haemadipsidae) are widely distributed in South East Asia. Haemadipsa crenata Ngamprasertwong is a blood-feeding species firstly reported from Thailand. A complete mitochondrial genome of H. crenata was characterized in this study for further genetic exploration on land leech. The reads were assembled into a circular mitogenome of 14,725 bp in length. The AT content of H. crenata mitogenome is 76.79%. The annotated mitogenome contains 22 tRNAs, 2 rRNAs, and 13 protein-coding genes (PCGs), and the structure of PCG open reading frames was confirmed. Finally, the phylogenetic relationship of H. crenata and other leech species were reconstructed using mitogenomes.

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supporting

confidence: 87%

Characterization of the complete mitogenome of a land leech, Haemadipsa crenata Ngamprasertwong (Arhynchobdellida: Haemadipsidae)

Wang

Tong

et al. 2021

Mitochondrial DNA Part B

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“…medicinalis and H . verbana have sometimes been confounded as a single species, they not only have distinct coloration and patterns, but also are genetically distinct, as shown through random amplified polymorphic DNA (RAPD) analyses [ 22 ] and mitochondrial genomic (mtDNA) sequencing [ 23 ]. While mating between the two species is possible, crosses between H .…”

Section: Introductionmentioning

confidence: 99%

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

et al. 2018

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The medicinal leech is one of the most venerated model systems for the study of fundamental nervous system principles, ranging from single-cell excitability to complex sensorimotor integration. Yet, molecular analyses have yet to be extensively applied to complement the rich history of electrophysiological study that this animal has received. Here, we generated the first de novo transcriptome assembly from the entire central nervous system of Hirudo verbana, with the goal of providing a molecular resource, as well as to lay the foundation for a comprehensive discovery of genes fundamentally important for neural function. Our assembly generated 107,704 contigs from over 900 million raw reads. Of these 107,704 contigs, 39,047 (36%) were annotated using NCBI’s validated RefSeq sequence database. From this annotated central nervous system transcriptome, we began the process of curating genes related to nervous system function by identifying and characterizing 126 unique ion channel, receptor, transporter, and enzyme contigs. Additionally, we generated sequence counts to estimate the relative abundance of each identified ion channel and receptor contig in the transcriptome through Kallisto mapping. This transcriptome will serve as a valuable community resource for studies investigating the molecular underpinnings of neural function in leech and provide a reference for comparative analyses.

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“…1a Table 3 and Supplementary Data 2. Additionally, we selected the mitochondrial contigs to assemble the leech mitochondrial genome [18].…”

Section: Genome Assembly and Annotationmentioning

confidence: 99%

Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches

et al. 2020

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Background Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. Results We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. Conclusions Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.

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Draft mitochondrial genomes of Hirudo medicinalis and Hirudo verbana (Annelida, Hirudinea).

Abstract: View related articles View Crossmark data Citing articles: 3 View citing articles MITOGENOME ANNOUNCEMENT Draft mitochondrial genomes of Hirudo medicinalis and Hirudo verbana (Annelida, Hirudinea).

Cited by 9 publications

References 7 publications

Characterization of the complete mitogenome of a land leech, Haemadipsa crenata Ngamprasertwong (Arhynchobdellida: Haemadipsidae)

Characterization of the complete mitogenome of a land leech, Haemadipsa crenata Ngamprasertwong (Arhynchobdellida: Haemadipsidae)

An annotated CNS transcriptome of the medicinal leech, Hirudo verbana: De novo sequencing to characterize genes associated with nervous system activity

Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches

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