Draft Genomes of Methanocalculus taiwanensis P2F9704a
 T
 and Methanocalculus chunghsingensis K1F9705b
 T
 , Hydrogenotrophic Methanogens Belonging to the Family
 Methanocalculaceae

Chen, Sheng-Chung; Lai, Shu-Jung; You, Yi-Ting; Shih, Chao-Jen; Wu, Yen-Chi; Wu, Chih-Hung; Liao, Ching-Hua

doi:10.1128/mra.00792-22

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Whole genome shotgun sequencing of strain CWC-04 T was performed at the Genomics BioSci and Tech Co., Ltd. (New Taipei City, Taiwan), using the MiSeq sequencer (Illumina). The library construction, barcoding and sequencing kit used were as described in our previous studies [39,40]. A total of 2 521 478 reads were generated and quality trimmed to obtain high-quality reads using Trimmomatic version 0.36 [41].…”

Section: Whole-genome Characterizationmentioning

confidence: 99%

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Zhang,

Lai,

Lin

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A novel mesophilic, hydrogenotrophic methanogen, strain CWC-04T, was obtained from a sediment sample extracted from a gravity core retrieved at station 22 within the KP-9 area off the southwestern coast of Taiwan during the ORIII-1368 cruise in 2009. Cells of strain CWC-04T were rod-shaped, 1.4–2.9 µm long by 0.5–0.6 µm wide, and occurred singly. Strain CWC-04Tutilized formate, H2/CO2, 2-propanol/CO2 or 2-butanol/CO2 as catabolic substrates. The optimal growth conditions were 42 °C, 0.17 M NaCl and pH 5.35. The genomic DNA G+C content calculated from the genome sequence of strain CWC-04T was 46.19 mol%. Phylogenetic analysis of 16S rRNA gene revealed that strain CWC-04T is affiliated with the genus Methanocella. The 16S rRNA gene sequences similarities within strains Methanocella arvoryzae MRE50T, Methanocella paludicola SANAET and Methanocella conradii HZ254T were 93.7, 93.0 and 91.3 %, respectively. In addition, the optical density of CWC-04T culture dropped abruptly upon entering the late-log growth phase, with virus-like particles (150 nm in diameter) being observed on and around the cells. This observation suggests that strain CWC-04T harbours a lytic virus. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CWC-04T represents a novel species of a novel genus in the family Methanocellaceae, for which the name Methanooceanicella nereidis gen. nov., sp. nov. is proposed. The type strain is CWC-04T (=BCRC AR10050T=NBRC 113165T).

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Section: Whole-genome Characterizationmentioning

confidence: 99%

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Zhang,

Lai,

Lin

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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“…The whole genome shotgun sequencing of these six strains examined in this study was conducted at Genomics BioSci and Tech Co., Ltd. (New Taipei City, Taiwan), employing the Illumina MiSeq sequencer. The library construction, barcoding and sequencing kit used were described in our previous studies [36,37].…”

Section: Whole-genome Characterizationmentioning

confidence: 99%

Six novel species of the genus Methanoculleus isolated from various environments in Taiwan

Lai,

Lin

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Taiwan is situated in the subtropical region and its geographical location and topographical features contribute to a rich ecological diversity and scenic landscapes. We investigated the diversity of methanogens in different environments of Taiwan using a culture-dependent method. This report presents the characterization and taxonomy of six hydrogenotrophic methanogens obtained from cold seep sediments (strain FWC-SCC1T and FWC-SCC3T), marine sediments (strain CWC-02T and YWC-01T), estuarine sediments (strain Afa-1T), and a hot spring well (strain Wushi-C6T) in Taiwan. The proposed names of the six novel species are Methanoculleus frigidifontis (type strain FWC-SCC1T=BCRC AR10056T=NBRC 113993T), Methanoculleus oceani (CWC-02T=BCRC AR10055T=NBRC 113992T), Methanoculleus methanifontis (FWC-SCC3T=BCRC AR10057T=NBRC 113994T), Methanoculleus nereidis (YWC-01T=BCRC AR10060T=NBRC 114597T), Methanoculleus formosensis (Afa-1T=BCRC AR10054T=NBRC 113995T), and Methanoculleus caldifontis (Wushi-06T=BCRC AR10059T= NBRC 114596T).

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Draft Genomes of Methanocalculus taiwanensis P2F9704a ^T and Methanocalculus chunghsingensis K1F9705b ^T , Hydrogenotrophic Methanogens Belonging to the Family Methanocalculaceae

Cited by 2 publications

References 17 publications

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Six novel species of the genus Methanoculleus isolated from various environments in Taiwan

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Draft Genomes of Methanocalculus taiwanensis P2F9704a T and Methanocalculus chunghsingensis K1F9705b T , Hydrogenotrophic Methanogens Belonging to the Family Methanocalculaceae

Cited by 2 publications

References 17 publications

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Methanooceanicella nereidis gen. nov., sp. nov., the first oceanic Methanocellaceae methanogen, isolated from potential methane hydrate bearing area offshore southwestern Taiwan

Six novel species of the genus Methanoculleus isolated from various environments in Taiwan

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Resources

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Draft Genomes of Methanocalculus taiwanensis P2F9704a ^T and Methanocalculus chunghsingensis K1F9705b ^T , Hydrogenotrophic Methanogens Belonging to the Family Methanocalculaceae