Draft Genome Sequences of Two Pseudomonas aeruginosa Multidrug-Resistant Clinical Isolates, PAL0.1 and PAL1.1

Grandjean, Teddy; Guern, Rémi Le; Duployez, Claire; Faure, Karine; Kipnis, Éric; Dessein, Rodrigue

doi:10.1128/mra.00940-18

Cited by 6 publications

(10 citation statements)

References 8 publications

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“…Several published studies on P . aeruginosa genome characterization have highlighted the prevalence of blaPAO and blaOXA50 which are classified as β-lactamases resistance genes [ 44 , 45 ]; and this does not make a difference in the P . aeruginosa strains under study.…”

Section: Discussionmentioning

confidence: 99%

“…The aph(3’)-IIb variant found in both UY1PASBAL and UY1PSABAL2 genome has been observed among MDR P . aeruginosa from several studies [ 44 , 45 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…This was further confirmed following the genomic analysis of the resistance genes (Table 2). Several published studies on P. aeruginosa genome characterization have highlighted the prevalence of blaPAO and blaOXA50 which are classified as β-lactamases resistance genes [44,45]; and this does not make a difference in the P. aeruginosa strains under study. In the study conducted by Girlich and colleagues [32], it was confirmed that insertion of blaOXA-50 into a P. aeruginosa host, decreases susceptibility to ampicillin, ticarcillin, moxalactam and meropenem.…”

Section: Antibiotic Resistancementioning

confidence: 99%

“…Apart from the rmtB gene, aminoglycosides modifying enzymes (AMEs) were found within UY1PASBAL and UY1PSABAL2 genomes, and it was exclusively aminoglycoses phosphotransferases (APHs) that mostly confer resistance to amikacin and isepamicin [49]. The aph(3')-IIb variant found in both UY1PASBAL and UY1PSABAL2 genome has been observed among MDR P. aeruginosa from several studies [44,45,50].…”

Section: Plos Onementioning

confidence: 99%

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Whole-genome sequence of multi-drug resistant Pseudomonas aeruginosa strains UY1PSABAL and UY1PSABAL2 isolated from human broncho-alveolar lavage, Yaoundé, Cameroon

et al. 2020

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Pseudomonas aeruginosa has been implicated in a wide range of post-operation wound and lung infections. A wide range of acquired resistance and virulence markers indicate surviving strategy of P. aeruginosa. Complete-genome analysis has been identified as efficient approach towards understanding the pathogenicity of this organism. This study was designed to sequence the entire genome of P. aeruginosa UY1PSABAL and UY1PSABAL2; determine drug-resistance profiles and virulence factors of the isolates; assess factors that contribute toward stability of the genomes; and thereafter determine evolutionary relationships between the strains and other isolates from similar sources. The genomes of the MDR P. aeruginosa UY1PSABAL and UY1PSABAL2 were sequenced on the Illumina Miseq platform. The raw sequenced reads were assessed for quality using FastQC v.0.11.5 and filtered for low quality reads and adapter regions using Trimmomatic v.0.36. The de novo genome assembly was made with SPAdes v.3.13 and annotated using Prokka v.2.1.1 annotation pipeline; Rapid Annotation using Subsytems Technology (RAST) server v.2.0; and PATRIC annotation tool v.3.6.2. Antimicrobial resistance genes and virulence determinants were searched through the functional annotation data generated from Prokka, RAST and PATRIC annotation pipelines; In addition to ResFinder and Comprehensive Antibiotic Resistance Database (CARD) which were employed to determine resistance genes. The PHAge Search Tool Enhanced Release (PHASTER) web server was used for the rapid identification and annotation of prophage sequences within bacterial genome. Predictive secondary metabolites were identified with AntiSMASH v.5.0. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and cas genes regions were also investigated with the CRISPRone and CRISPRFinder server. The genome sizes of 7.0 and 6.4 Mb were

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Section: Discussionmentioning

confidence: 99%

“…The aph(3’)-IIb variant found in both UY1PASBAL and UY1PSABAL2 genome has been observed among MDR P . aeruginosa from several studies [ 44 , 45 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Antibiotic Resistancementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

See 2 more Smart Citations

Whole-genome sequence of multi-drug resistant Pseudomonas aeruginosa strains UY1PSABAL and UY1PSABAL2 isolated from human broncho-alveolar lavage, Yaoundé, Cameroon

et al. 2020

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“…As mentioned before, the resistome analysis revealed that the five isolates harbored the beta-lactamase genes bla OXA-396 and bla PAO . Previous studies demonstrated the high prevalence of these genes in P. aeruginosa isolates from different sources and their contribution to antimicrobial resistance in this species [ 23 , 24 , 25 , 26 , 27 ]. According to the ResFinder tool on WGS from the five CF isolates, no carbapenemase genes were detected.…”

Section: Resultsmentioning

confidence: 99%

New Sequence Type ST3449 in Multidrug-Resistant Pseudomonas aeruginosa Isolates from a Cystic Fibrosis Patient

et al. 2021

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Pseudomonas aeruginosa is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of P. aeruginosa collected during a month from a CF patient with end-stage lung disease and fatal outcome. The isolates exhibited distinct colony morphologies and pigmentation and differences in their capacity to produce biofilm and virulence potential evaluated in larvae of Galleria mellonella. Whole genome-sequencing showed that isolates belonged to a novel sequence type ST3449 and serotype O6. Analysis of their resistome demonstrated the presence of genes blaOXA-396, blaPAO, aph(3’)-IIb, catB, crpP and fosA and new mutations in chromosomal genes conferring resistance to different antipseudomonal antibiotics. Genes exoS, exoT, exoY, toxA, lasI, rhlI and tse1 were among the 220 virulence genes detected. The different phenotypic and genotypic features found reveal the adaptation of clone ST3449 to the CF lung environment by a number of mutations affecting genes related with biofilm formation, quorum sensing and antimicrobial resistance. Most of these mutations are commonly found in CF isolates, which may give us important clues for future development of new drug targets to combat P. aeruginosa chronic infections.

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Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

et al. 2022

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Pseudomonas aeruginosa biofilms cause chronic, antibiotic tolerant infections in wounds and lungs. Numerous recent studies demonstrate that bacteria can detect human communication compounds through specific sensor/receptor tools that modulate bacterial physiology. Consequently, interfering with these mechanisms offers an exciting opportunity to directly affect the infection process. It is shown that the human hormone Atrial Natriuretic Peptide (hANP) both prevents the formation of P. aeruginosa biofilms and strongly disperses established P. aeruginosa biofilms. This hANP action is dose‐dependent with a strong effect at low nanomolar concentrations and takes effect in 30–120 min. Furthermore, although hANP has no antimicrobial effect, it acts as an antibiotic adjuvant. hANP enhances the antibiofilm action of antibiotics with diverse modes of action, allowing almost full biofilm eradication. The hANP effect requires the presence of the P. aeruginosa sensor AmiC and the AmiR antiterminator regulator, indicating a specific mode of action. These data establish the activation of the ami pathway as a potential mechanism for P. aeruginosa biofilm dispersion. hANP appears to be devoid of toxicity, does not enhance bacterial pathogenicity, and acts synergistically with antibiotics. These data show that hANP is a promising powerful antibiofilm weapon against established P. aeruginosa biofilms in chronic infections.

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Draft Genome Sequences of Two Pseudomonas aeruginosa Multidrug-Resistant Clinical Isolates, PAL0.1 and PAL1.1

Cited by 6 publications

References 8 publications

Whole-genome sequence of multi-drug resistant Pseudomonas aeruginosa strains UY1PSABAL and UY1PSABAL2 isolated from human broncho-alveolar lavage, Yaoundé, Cameroon

Whole-genome sequence of multi-drug resistant Pseudomonas aeruginosa strains UY1PSABAL and UY1PSABAL2 isolated from human broncho-alveolar lavage, Yaoundé, Cameroon

New Sequence Type ST3449 in Multidrug-Resistant Pseudomonas aeruginosa Isolates from a Cystic Fibrosis Patient

Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

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