Draft Genome Sequences of Three Northern German Epidemic Staphylococcus aureus (ST247) Strains Containing Multiple Copies of IS
            <i>256</i>

Kleinert, Franziska; Kallies, René; Zweynert, Annegret; Bierbaum, Gabriele

doi:10.1128/genomea.00936-16

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Some authors reported that despite the presence of ica operon, some staphylococcal isolates produce weak biofilm production due to the inactivation of icaA by insertion of IS256 ( Cho et al, 2002 ; Kiem et al, 2004 ). Further reported that the insertion of IS256 inactivates mutS and contributes to vancomycin resistance development in vancomycin-intermediate S. aureus strains ( Kleinert et al, 2016 ). Also, the upregulation of icaA and icaD genes during acidic stress promotes biofilm formation which in-turn plays a role to resist it from acidic and alkaline environments and establishes the niche adaptation in Staphylococcus strains ( Lindsay et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Naorem

Blom

Fekete

2021

PeerJ

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Staphylococcus aureus is a drug-resistant pathogen, capable of colonizing diverse ecological niches and causing a broad spectrum of infections related to a community and healthcare. In this study, we choose four methicillin-resistant S. aureus (MRSA) clinical isolates from Germany and Hungary based on our previous polyphasic characterization finding. We assumed that the selected strains have a different genetic background in terms of the presence of resistance and virulence genes, prophages, plasmids, and secondary metabolite biosynthesis genes that may play a crucial role in niche adaptation and pathogenesis. To clarify these assumptions, we performed a comparative genome analysis of these strains and observed many differences in their genomic compositions. The Hungarian isolates (SA H27 and SA H32) with ST22-SCCmec type IVa have fewer genes for multiple-drug resistance, virulence, and prophages reported in Germany isolates. Germany isolate, SA G6 acquires aminoglycoside (ant(6)-Ia and aph(3’)-III) and nucleoside (sat-4) resistance genes via phage transduction and may determine its pathogenic potential. The comparative genome study allowed the segregation of isolates of geographical origin and differentiation of the clinical isolates from the commensal isolates. This study suggested that Germany and Hungarian isolates are genetically diverse and showing variation among them due to the gain or loss of mobile genetic elements (MGEs). An interesting finding is the addition of SA G6 genome responsible for the drastic decline of the core/pan-genome ratio curve and causing the pan-genome to open wider. Functional characterizations revealed that S. aureus isolates survival are maintained by the amino acids catabolism and favor adaptation to growing in a protein-rich medium. The dispersible and singleton genes content of S. aureus genomes allows us to understand the genetic variation among the CC5 and CC22 groups. The strains with the same genetic background were clustered together, which suggests that these strains are highly alike; however, comparative genome analysis exposed that the acquisition of phage elements, and plasmids through the events of MGEs transfer contribute to differences in their phenotypic characters. This comparative genome analysis would improve the knowledge about the pathogenic S. aureus strain’s characterization, and responsible for clinically important phenotypic differences among the S. aureus strains.

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Section: Discussionmentioning

confidence: 99%

Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Naorem

Blom

Fekete

2021

PeerJ

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“…The genomic sequences of the ST247 strains S. aureus SA1450/94, SA137/93A, and SA137/93G were determined by next-generation sequencing (NGS) using 454 sequencing technology (54). In the first de novo assembly of the NGS reads with the GS De Novo Assembler (Roche Applied Science GmbH, Mannheim, Germany), the sequences of the IS elements IS256, IS431mec, and IS1181 were recognized as repetitive sequences and were summarized as three single contigs.…”

Section: Is256 and Scv Formation In St247 S Aureus Antimicrobial Agementioning

confidence: 99%

“…Accession number(s). The sequences have been deposited in GenBank with the following accession numbers: for S. aureus SA137/93A, JWMH00000000 (JWMH01000001 to JWMH01000036); for S. aureus SA137/93G, JWMG00000000 (JWMG01000001 to JWMG01000041); and for S. aureus SA1450/94, JWMI00000000 (JWMI01000001 to JWMI01000029) (54).…”

Section: Is256 and Scv Formation In St247 S Aureus Antimicrobial Agementioning

confidence: 99%

Influence of IS 256 on Genome Variability and Formation of Small-Colony Variants in Staphylococcus aureus

Kleinert

Kallies

Hort

et al. 2017

Antimicrob Agents Chemother

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Staphylococcus aureus has acquired resistance to nearly all antibiotics used in clinical practice. Whereas some resistance mechanisms are conferred by uptake of resistance genes, others evolve by mutation. In this study, IS256 has been shown to play a role, e.g., in S. aureus strains displaying intermediate resistance to vancomycin (VISA). To characterize the IS256 insertion sites in the genomes of two closely related sequence type 247 (ST247) VISA strains, all insertions were mapped in both VISA and a susceptible control strain. The results showed that the three ST247 strains contained the highest number so far of IS256 insertions for all sequenced S. aureus strains. Furthermore, in contrast to the case with the other IS elements in these genomes, the IS256 insertion sites were not identical in the closely related strains, indicating a high transposition frequency of IS256. When IS256 was introduced into a laboratory strain which was then cultured in the presence of antibiotics, it was possible to isolate small-colony variants (SCVs) that possessed IS256 insertions in guaA and hemY that displayed increased resistance to vancomycin and aminoglycosides, respectively. For these clones, a very rapid reversion to the wild type that resembled the fast reversion of clinical SCVs was observed. The reversion was caused by excision of IS256 in a small number of fast-growing clones that quickly outcompeted the SCVs in broth cultures. In conclusion, the presence of IS256 confers a strong genomic plasticity that is useful for adaptation to antibiotic stress.

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“…Some authors reported that despite the presence of ica operon, some staphylococcal isolates produce weak biofilm production due to the inactivation of icaA by insertion of IS256 (Cho et al, 2002;Kiem et al, 2004). Further reported that the insertion of IS256 inactivates mutS and contributes to vancomycin resistance development in vancomycin-intermediate S. aureus strains (Kleinert et al, 2016). Also, the upregulation of icaA and icaD genes during acidic stress promotes biofilm formation which in-turn plays a role to resist it from acidic and alkaline environments and establishes the niche adaptation in Staphylococcus strains (Lindsay et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Figure 2: Heat map showing the presence (red color) and absence (blue color) of antibiotic resistance genes.

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Draft Genome Sequences of Three Northern German Epidemic Staphylococcus aureus (ST247) Strains Containing Multiple Copies of IS 256

Cited by 4 publications

References 11 publications

Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Genome-wide comparison of four MRSA clinical isolates from Germany and Hungary

Influence of IS 256 on Genome Variability and Formation of Small-Colony Variants in Staphylococcus aureus

Figure 2: Heat map showing the presence (red color) and absence (blue color) of antibiotic resistance genes.

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