Draft Genome Sequences of Isolates from Sediments of the River Elbe That Are Highly Tolerant to Diclofenac

Quesada, José Miguel; Aguilar-Romero, Inés; Torre, J. de la; Wittich, Rolf-Michael; Dillewijn, Pieter van

doi:10.1128/mra.00849-18

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…Pseudomonas sp. strain RW407 was isolated from polluted sediment of the Elbe River in Germany, Pseudomonas citronellolis strain P3B5 was isolated from the basil plant phyllosphere, and strain 273 was obtained from garden soil collected in the Stuttgart area, Germany. , The high sequence similarity between alkB -2 of strain 273 and the alkB genes of strain RW407 and strain P3B5 could indicate horizontal gene transfer, an observation supported by a recent study that analyzed alkB genes in a genomic context and concluded that horizontal gene transfer events contribute substantially to the dissemination of alkB genes …”

Section: Discussionmentioning

confidence: 72%

Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism in Pseudomonas sp. Strain 273

Xie,

Ramirez,

Chen

et al. 2023

Environ. Sci. Technol.

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Pseudomonas sp. strain 273 grows with medium-chain terminally fluorinated alkanes under oxic conditions, releases fluoride, and synthesizes long-chain fluorofatty acids. To shed light on the genes involved in fluoroalkane metabolism, genome, and transcriptome sequencing of strain 273 grown with 1,10-difluorodecane (DFD), decane, and acetate were performed. Strain 273 harbors three genes encoding putative alkane monooxygenases (AlkB), key enzymes for initiating alkane degradation. Transcripts of alkB-2 were significantly more abundant in both decane- and DFD-grown cells compared to acetate-grown cells, suggesting AlkB-2 catalyzes the attack on terminal CH3 and CH2F groups. Coordinately expressed with alkB-2 was an adjacent gene encoding a fused ferredoxin–ferredoxin reductase (Fd–Fdr). Phylogenetic analysis distinguished AlkB that couples with fused Fd–Fdr reductases from AlkB with alternate architectures. A gene cluster containing an (S)-2-haloacid dehalogenase (had) gene was up-regulated in cells grown with DFD, suggesting a possible role in the removal of the ω-fluorine. Genes involved in long-chain fatty acid biosynthesis were not differentially expressed during growth with acetate, decane, or DFD, suggesting the bacterium’s biosynthetic machinery does not discriminate against monofluoro-fatty acid intermediates. The analysis sheds first light on genes and catalysts involved in the microbial metabolism of fluoroalkanes.

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Section: Discussionmentioning

confidence: 72%

Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism in Pseudomonas sp. Strain 273

Xie,

Ramirez,

Chen

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

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Draft Genome Sequences of Isolates from Sediments of the River Elbe That Are Highly Tolerant to Diclofenac

Abstract: Here, we report the genome sequences of one Achromobacter and four Pseudomonas strains isolated from sediments of the River Elbe which are highly tolerant toward the xenobiotic target compound diclofenac, a nonsteroidal anti-inflammatory drug (NSAID) and emerging contaminant.

Cited by 1 publication

References 13 publications

Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism in Pseudomonas sp. Strain 273

Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism in Pseudomonas sp. Strain 273

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