Draft Genome Sequences of Helicobacter pylori Isolates from Malaysia, Cultured from Patients with Functional Dyspepsia and Gastric Cancer

Gunaletchumy, S.P.; Teh, Xinsheng; Khosravi, Yalda; Ramli, Norlisah; Chua, Eng Guan; Thevakumar, Kavitha; Mason, Joanne N.; Lee, HueyTyng; Alias, Hamidah; Zaidan, Nur Zafirah; Yassin, Norzawani Buang M.; Tay, Liang Chung; Rudd, Stephen; Mitchell, Hazel M.; Kaakoush, Nadeem O.; Loke, Mun Fai; Goh, Khean Lee; Vadivelu, Jamuna

doi:10.1128/jb.01278-12

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…The H pylori strain GC026 ( cagA +, cagE +, cagL +, cagT +, vacA s1 m1+, babA +, oipA +, dupA + and sabA +) was isolated from a GC patient at the University Hospital of Malaysia, Kuala Lumpur [21] , [22] . The H. pylori strain 26695 ( cagA + and vacA s1m1+) was isolated from a patient with gastritis [23] (ATCC code 700392).…”

Section: Methodsmentioning

confidence: 99%

The NOD-Like Receptor Signalling Pathway in Helicobacter pylori Infection and Related Gastric Cancer: A Case-Control Study and Gene Expression Analyses

et al. 2014

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BackgroundCurrently, it is well established that cancer arises in chronically inflamed tissue. A number of NOD-like receptors (NLRs) form inflammasomes, intracellular multiprotein complexes critical for generating mature pro-inflammatory cytokines (IL-1β and IL-18). As chronic inflammation of the gastric mucosa is a consequence of Helicobacter pylori infection, we investigated the role of genetic polymorphisms and expression of genes involved in the NLR signalling pathway in H. pylori infection and related gastric cancer (GC).Materials and MethodsFifty-one genetic polymorphisms were genotyped in 310 ethnic Chinese (87 non-cardia GC cases and 223 controls with functional dyspepsia). In addition, gene expression of 84 molecules involved in the NLR signalling pathway was assessed in THP-1 cells challenged with two H. pylori strains, GC026 (GC) and 26695 (gastritis).Results CARD8-rs11672725, NLRP3-rs10754558, NLRP3-rs4612666, NLRP12-rs199475867 and NLRX1-rs10790286 showed significant associations with GC. On multivariate analysis, CARD8-rs11672725 remained a risk factor (OR: 4.80, 95% CI: 1.39–16.58). Further, NLRP12-rs2866112 increased the risk of H. pylori infection (OR: 2.13, 95% CI: 1.22–3.71). Statistical analyses assessing the joint effect of H. pylori infection and the selected polymorphisms revealed strong associations with GC (CARD8, NLRP3, CASP1 and NLRP12 polymorphisms). In gene expression analyses, five genes encoding NLRs were significantly regulated in H. pylori-challenged cells (NLRC4, NLRC5, NLRP9, NLRP12 and NLRX1). Interestingly, persistent up-regulation of NFKB1 with simultaneous down-regulation of NLRP12 and NLRX1 was observed in H. pylori GC026-challenged cells. Further, NF-κB target genes encoding pro-inflammatory cytokines, chemokines and molecules involved in carcinogenesis were markedly up-regulated in H. pylori GC026-challenged cells.ConclusionsNovel associations between polymorphisms in the NLR signalling pathway (CARD8, NLRP3, NLRP12, NLRX1, and CASP1) and GC were identified in Chinese individuals. Our genetic polymorphisms and gene expression results highlight the relevance of the NLR signalling pathway in gastric carcinogenesis and its close interaction with NF-κB.

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Section: Methodsmentioning

confidence: 99%

The NOD-Like Receptor Signalling Pathway in Helicobacter pylori Infection and Related Gastric Cancer: A Case-Control Study and Gene Expression Analyses

et al. 2014

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“…The H. pylori strain 26695 (cagA+ and vacA s1m1+) was isolated from a patient with gastritis (code: ATCC 700392) . The H. pylori strain GC026 (cagA+, cagE+, cagL+, cagT+, vacA s1m1+, babA+, oipA+, dupA+, and sabA+) was isolated from a GC patient undergoing endoscopy at the University Hospital of Malaysia, Kuala Lumpur, in a previous study conducted by our group . Both H. pylori strains were grown for 48 hours on horse blood agar plates (Blood Agar Base No.…”

Section: Methodsmentioning

confidence: 99%

Autophagy in Helicobacter pylori Infection and Related Gastric Cancer

et al. 2015

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“…Recent sequencing efforts have reported multiple genome sequences of H. pylori isolates from patients of different ethnicities and various disease manifestations from Malaysia ( 21 – 23 ). To date, there are 29 Malaysian H. pylori genomes (27 clinical strains and two mice-adapted strains) available in NCBI database, a majority of them sequenced and deposited as a part of this work.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of Helicobacter pylori from Malaysia identifies three distinct lineages suggestive of differential evolution

Kumar

Mariappan

Baddam

et al. 2014

Nucleic Acids Research

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The discordant prevalence of Helicobacter pylori and its related diseases, for a long time, fostered certain enigmatic situations observed in the countries of the southern world. Variation in H. pylori infection rates and disease outcomes among different populations in multi-ethnic Malaysia provides a unique opportunity to understand dynamics of host–pathogen interaction and genome evolution. In this study, we extensively analyzed and compared genomes of 27 Malaysian H. pylori isolates and identified three major phylogeographic lineages: hspEastAsia, hpEurope and hpSouthIndia. The analysis of the virulence genes within the core genome, however, revealed a comparable pathogenic potential of the strains. In addition, we identified four genes limited to strains of East-Asian lineage. Our analyses identified a few strain-specific genes encoding restriction modification systems and outlined 311 core genes possibly under differential evolutionary constraints, among the strains representing different ethnic groups. The cagA and vacA genes also showed variations in accordance with the host genetic background of the strains. Moreover, restriction modification genes were found to be significantly enriched in East-Asian strains. An understanding of these variations in the genome content would provide significant insights into various adaptive and host modulation strategies harnessed by H. pylori to effectively persist in a host-specific manner.

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Draft Genome Sequences of Helicobacter pylori Isolates from Malaysia, Cultured from Patients with Functional Dyspepsia and Gastric Cancer

Cited by 7 publications

References 8 publications

The NOD-Like Receptor Signalling Pathway in Helicobacter pylori Infection and Related Gastric Cancer: A Case-Control Study and Gene Expression Analyses

The NOD-Like Receptor Signalling Pathway in Helicobacter pylori Infection and Related Gastric Cancer: A Case-Control Study and Gene Expression Analyses

Autophagy in Helicobacter pylori Infection and Related Gastric Cancer

Comparative genomic analysis of Helicobacter pylori from Malaysia identifies three distinct lineages suggestive of differential evolution

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