Draft Genome Sequences of Four Pseudomonas aeruginosa Clinical Strains with Various Biofilm Phenotypes

Boukerb, Amine M.; Simon, Marjolaine; Pernet, Erwan; Jouault, Albane; Portier, Emilie; Persyn, Elise; Bouffartigues, Emeline; Bazire, Alexis; Chevalier, Sylvie; Feuilloley, Marc; Lesouhaitier, Olivier; Caillon, Jocelyne; Dufour, Alain

doi:10.1128/mra.01286-19

Cited by 6 publications

(6 citation statements)

References 9 publications

(5 reference statements)

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“…An alternative hypothesis is that the architecture of the biofilm, rather than the organization of the ami operon, may explain the strain specific differences in OSTN susceptibility. Indeed, characterization of the clinical strains selected in the panel demonstrated that they form biofilms with significant differences in biofilm spatial organization and matrix composition [ 50 , 71 ]. Moreover, it was recently observed that P. aeruginosa PA14 exposed to hANP modifies the exopolysaccharides composition of the biofilm matrix [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…P. aeruginosa PAK strain is a non-mucoid clinical strain [ 49 ]. Strains MUC-N1, MUC-N2, MUC-P4, MUC-P5 [ 50 ] are clinical isolates collected from sputum of CF patients (Nantes hospital) as well as CF6.14 (CNR Besançon, France). Finally, the clinical strains PAL 0.1 and PAL 1.1 [ 51 ] were isolated from the respiratory tract of a patient in intensive care unit (Lille hospital, France).…”

Section: Methodsmentioning

confidence: 99%

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The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm

Louis¹,

Tahrioui²,

Lendon

et al. 2023

Biofilm

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm

Louis¹,

Tahrioui²,

Lendon

et al. 2023

Biofilm

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“…P. aeruginosa MUC-N1 is a mucoid clinical isolated from a cystic fibrosis patient at the Nantes teaching hospital [ 28 , 37 ]. P. aeruginosa MUC N1 was grown at 37 °C in Luria-Bertani medium (NaCl 10 g/L, tryptone 10 g/L, and yeast extract 5 g/L) under orbital shaking (125 rpm).…”

Section: Methodsmentioning

confidence: 99%

Extracts from Wallis Sponges Inhibit Vibrio harveyi Biofilm Formation

et al. 2023

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Pathogenic bacteria and their biofilms are involved in many human and animal diseases and are a major public health problem with, among other things, the development of antibiotic resistance. These biofilms are known to induce chronic infections for which classical treatments using antibiotic therapy are often ineffective. Sponges are sessile filter-feeding marine organisms known for their dynamic symbiotic partnerships with diverse microorganisms and their production of numerous metabolites of interest. In this study, we investigated the antibiofilm efficacy of different extracts from sponges, isolated in Wallis, without biocidal activity. Out of the 47 tested extracts, from 28 different genera, 11 showed a strong activity against Vibrio harveyi biofilm formation. Moreover, one of these extracts also inhibited two quorum-sensing pathways of V. harveyi.

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“…[ 71 ] The MUC‐N1, MUC‐N2, MUC‐P4, and MUC‐P5 P. aeruginosa strains were isolated from sputum samples of adult cystic fibrosis patients followed at the Centre de Référence Contre la Mucoviscidose (CRCM), Centre Hospitalier Universitaire (CHU) of Nantes (France). [ 72 ] The P. aeruginosa clinical strains CF 8.19, CF 9.19 were isolated from sputum of cystic fibrosis patients (CF 8.19 and CF 9.19) (CNR résistance aux antibiotiques CHU Besançon, France). PAL 0.1 and PAL 1.1 were isolated from the airways of an intensive care unit (ICU) patient (Lille CHU hospital, France), with ventilator‐associated pneumonia (PAL1.1), or isolated from blood cultures of an ICU patient with sepsis PAL0.1.…”

Section: Methodsmentioning

confidence: 99%

Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

et al. 2022

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Pseudomonas aeruginosa biofilms cause chronic, antibiotic tolerant infections in wounds and lungs. Numerous recent studies demonstrate that bacteria can detect human communication compounds through specific sensor/receptor tools that modulate bacterial physiology. Consequently, interfering with these mechanisms offers an exciting opportunity to directly affect the infection process. It is shown that the human hormone Atrial Natriuretic Peptide (hANP) both prevents the formation of P. aeruginosa biofilms and strongly disperses established P. aeruginosa biofilms. This hANP action is dose‐dependent with a strong effect at low nanomolar concentrations and takes effect in 30–120 min. Furthermore, although hANP has no antimicrobial effect, it acts as an antibiotic adjuvant. hANP enhances the antibiofilm action of antibiotics with diverse modes of action, allowing almost full biofilm eradication. The hANP effect requires the presence of the P. aeruginosa sensor AmiC and the AmiR antiterminator regulator, indicating a specific mode of action. These data establish the activation of the ami pathway as a potential mechanism for P. aeruginosa biofilm dispersion. hANP appears to be devoid of toxicity, does not enhance bacterial pathogenicity, and acts synergistically with antibiotics. These data show that hANP is a promising powerful antibiofilm weapon against established P. aeruginosa biofilms in chronic infections.

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Draft Genome Sequences of Four Pseudomonas aeruginosa Clinical Strains with Various Biofilm Phenotypes

Cited by 6 publications

References 9 publications

The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm

The natriuretic peptide receptor agonist osteocrin disperses Pseudomonas aeruginosa biofilm

Extracts from Wallis Sponges Inhibit Vibrio harveyi Biofilm Formation

Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

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