Draft Genome Sequences of Four Novel Strains of Microbes Isolated from
            <i>Lepidocephalichthys guntea</i>

Basak, Chandana; Mondal, Nibendu; Sen, Subhajit; Sarkar, Jagannath; Ghosh, Wriddhiman; Chakraborty, Ranadhir

doi:10.1128/mra.00621-21

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The DO 2 in the control tank was kept at 7 ± 0.5 ppm throughout the experiment. Fish from the control tank was similarly collected, euthanized, and their guts dissected under aseptic conditions after 38 h, as per standard protocol (Basak et al 2021). Each sh's dissected gut, whether control or experimental, was further chopped into two parts, representing the anterior and posterior regions.…”

Section: Design Of Experimentsmentioning

confidence: 99%

Fluctuation of gut microbiome in Cirrhinus mrigala is associated with changes in dissolved oxygen of water

Basak

Mondal

Chatterjee

et al. 2022

Preprint

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Oxygen depletion, or hypoxia, a common consequence of eutrophication, has an adverse effect on aquatic flora and fauna. Bacteria living within or around aquatic macroorganisms struggle to thrive in low dissolved oxygen (DO2) conditions. The goal of our study was to see how the bacterial population in the intestine of a bottom-feeding fish, Cirrhinus mrigala, fluctuated when the DO2 level in the water decreased from 7±0.5 ppm to 0.35± 0.05 ppm. The V3 regions of 16S rRNA genes derived out of metagenome extracted from the intestines of control and experimental fishes were sequenced and analysed. The findings revealed that during DO2 stress (0.35±0.05 ppm), the aerobic bacterial population in the gut (existing when fish lived in 7±0.5 ppm DO2) changed significantly, transforming the microbial community into a facultative and/or obligate anaerobic community with the dominance of microaerophilic bacterial populations; density of Cetobacterium increased in intestinal samples, which is linked to the prevention of paradoxical anaerobicity in fish tissues. The current research advances our understanding of the changes in gut microbiome ecology of intestinal bacterial flora under DO2 stress.

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Section: Design Of Experimentsmentioning

confidence: 99%

Fluctuation of gut microbiome in Cirrhinus mrigala is associated with changes in dissolved oxygen of water

Basak

Mondal

Chatterjee

et al. 2022

Preprint

Self Cite

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A novel strain of Shigella isolated from the gut of Lepidocephalichthys guntea has in its genome a complete gene package for Type ll secretion system, and elaborate repertoire of genes responsible for multiple antibiotic-resistance and metal resistance via specific efflux channels

Basak

Chakraborty

2022

Letters in Applied Microbiology

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The bacterial strain GCP5 was isolated from the gut of a bottom-dwelling fish Lepidocephalichthys guntea, that lives in the Magurmari River near North Bengal University in Siliguri, India. GCP5 was phylogenetically assigned to the Shigella genus using whole genome-based trees, k-mer analysis, the multilocus species tree (MLST), and single nucleotide polymorphism (SNP)-based trees, and the genetic makeup of the isolate was determined following assembly of the genome sequences and genome annotation with several bioinformatics tools. The presence of a complete package of general-secretory-pathway (gsp) genes, grouped in an operon identical to a well-characterized type II secretion system (T2SS), was confirmed by genome mining of Shigella sp. GCP5. The operon’s gsp genes shared the most homology with Escherichia coli gsp genes. A few more high-pathogenicity islands (HPIs) in the GCP5 genome were validated using the pan-genomes analysis pipeline (PGAP) and island viewer. Several antibiotic-resistance genes were found in this genome, as well as the existence of key antibiotic efflux pump families, allowing for the creation of a gene network of several antibiotic efflux transporters. In addition, the genome contained genes specific for nickel transport, the nikABCD system, and the RND family transporter cusCFBA, which confers resistance to copper and silver by effluxing out Cu+ and Ag+ ions.

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Draft Genome Sequences of Four Novel Strains of Microbes Isolated from Lepidocephalichthys guntea

Cited by 2 publications

References 11 publications

Fluctuation of gut microbiome in Cirrhinus mrigala is associated with changes in dissolved oxygen of water

Fluctuation of gut microbiome in Cirrhinus mrigala is associated with changes in dissolved oxygen of water

A novel strain of Shigella isolated from the gut of Lepidocephalichthys guntea has in its genome a complete gene package for Type ll secretion system, and elaborate repertoire of genes responsible for multiple antibiotic-resistance and metal resistance via specific efflux channels

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