Draft Genome Sequences of Corynebacterium kroppenstedtii CNM633/14 and CNM632/14, Multidrug-Resistant and Antibiotic-Sensitive Isolates from Nodules of Granulomatous Mastitis Patients

Fernández-Natal, María Isabel; Soriano, Francisco; Ariza-Miguel, Jaime; Marrodan-Ciordia, Teresa; Acedo, Alberto; Hernández, Marta; Tauch, Andreas; Rodrı́guez-Lázaro, David

doi:10.1128/genomea.00525-15

Cited by 8 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the genomic island includes all insertion sequences previously described in pJA144188. The gene content of the resistance island is fully consistent with the antimicrobial susceptibility data (Table 1), as erm (X), tet (W), cmx, aphA1-IAB, strAB and sul1 can confer resistances to MLSb antibiotics, tetracyclines, chloramphenicol, aminoglycosides, streptomycin and sulfonamides in corynebacteria [24].…”

Section: Discussionsupporting

confidence: 80%

“…Definitive identification was also obtained by sequencing the 16S rRNA gene [18], [22], and our two strains presented nucleotide sequence similarities with GenBank sequences of C. kroppenstedtii from 99 to 100%. The species identification was furthermore corroborated by genome sequencing and the deduced genomic similarity of both strains to the type strain C. kroppenstedtii DSM 44385 [2], [24].…”

Section: Discussionmentioning

confidence: 76%

“…The phenotypical novel multidrug resistance of C. kroppenstedtii CNM633/14 was confirmed when the genome sequence was analysed and several antibiotic resistance genes were annotated [24]. Interestingly, most of them are located in a specific genomic island with similarity to the R plasmid pJA144188, conferring multidrug resistance to C. resistens DSM 45100 [26].…”

Section: Discussionmentioning

confidence: 90%

See 2 more Smart Citations

Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis

Fernández-Natal

Rodrı́guez-Lázaro

Marrodan-Ciordia

et al. 2016

New Microbes and New Infections

View full text Add to dashboard Cite

Human infections associated with Corynebacterium kroppenstedtii are rarely reported, and this organism is usually described as antibiotic sensitive. Almost all published cases of C. kroppenstedtii infections have been associated with breast pathology in women and have been described in New Zealand, France, Canada, India and Japan. Here we describe the microbiologic characteristics of two strains isolated from two women diagnosed of granulomatous mastitis in Spain. One C. kroppenstedtii isolate was antibiotic sensitive while the other was multidrug resistant. Biochemical identification was possible using a wide battery of methods including API Coryne V2.0, API Strep, API NH, API NE, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene amplification and sequencing. Antimicrobial susceptibility to 28 antibiotics as determined by Etest showed one isolate being sensitive to benzylpenicillin, ciprofloxacin, moxifloxacin, gentamicin, vancomycin, clindamycin, tetracycline, linezolid and rifampin. The second isolate showed resistance to ciprofloxacin, moxifloxacin, clindamycin, tetracycline and rifampin. The multidrug-resistant isolate contained the erm(X), tet(W), cmx, aphA1-IAB, strAB and sul1 resistance genes known from the R plasmid pJA144188 of Corynebacterium resistens. These genes were absent in the genome of the antibiotic-sensitive isolate. This report confirms the tropism of this microorganism for women's breasts and presents the first description of a multidrug-resistant C. kroppenstedtii strain.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 90%

See 1 more Smart Citation

Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis

Fernández-Natal

Rodrı́guez-Lázaro

Marrodan-Ciordia

et al. 2016

New Microbes and New Infections

View full text Add to dashboard Cite

show abstract

“…Some well-known representatives include C. glutamicum, which (like the thermostable C. efficiens) is widely used in industry for the production of amino acids such as L-glutamic acid and L-lysine for human and animal nutrition, respectively (118). Several genome sequences of Corynebacterium species have been reported, including those of C. ulcerans (119), C. kutscheri (120), C. kroppenstedtii (121), and C. argentoratense (122), providing important new insights into the genomic architecture of the genus. A prophage, CGP3, that integrates into the genome of C. glutamicum and encodes an actin-like protein, AlpC, was recently described (123).…”

Section: Figmentioning

confidence: 99%

Taxonomy, Physiology, and Natural Products of Actinobacteria

Barka

Vatsa

Sanchez

et al. 2016

Microbiol Mol Biol Rev

1,524

1,048

View full text Add to dashboard Cite

SUMMARYActinobacteriaare Gram-positive bacteria with high G+C DNA content that constitute one of the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and terrestrial ecosystems. ManyActinobacteriahave a mycelial lifestyle and undergo complex morphological differentiation. They also have an extensive secondary metabolism and produce about two-thirds of all naturally derived antibiotics in current clinical use, as well as many anticancer, anthelmintic, and antifungal compounds. Consequently, these bacteria are of major importance for biotechnology, medicine, and agriculture.Actinobacteriaplay diverse roles in their associations with various higher organisms, since their members have adopted different lifestyles, and the phylum includes pathogens (notably, species ofCorynebacterium,Mycobacterium,Nocardia,Propionibacterium, andTropheryma), soil inhabitants (e.g.,MicromonosporaandStreptomycesspecies), plant commensals (e.g.,Frankiaspp.), and gastrointestinal commensals (Bifidobacteriumspp.).Actinobacteriaalso play an important role as symbionts and as pathogens in plant-associated microbial communities. This review presents an update on the biology of this important bacterial phylum.

show abstract

“…In 2015, draft genome sequences of the antibiotic-sensitive strain C. kroppenstedtii ITA105 (CNM633/14) and the multidrugresistant strain C. kroppenstedtii ITA205 (CNM632/14) were determined, both isolated from cases of granulomatous mastitis. 41 The current three genome sequences show that the C. kroppenstedtii chromosome has a size range of 2.44 Mbp to 2.57 Mbp and a chromosomal G + C content ranging from 56.8% to 57.5%. The number of protein-coding genes predicted by bioinformatic methods is in the range of 2000 ( Table 3).…”

Section: Genome Sequences and Antibiotic Resistancementioning

confidence: 85%

A microbiological and clinical review on Corynebacterium kroppenstedtii

Tauch

Fernández-Natal

Soriano³

2016

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

The genus Corynebacterium represents a taxon of Gram-positive bacteria with a high G+C content in the genomic DNA. Corynebacterium kroppenstedtii is an unusual member of this taxon as it lacks the characteristic mycolic acids in the cell envelope. Genome sequence analysis of the C. kroppenstedtii type strain has revealed a lipophilic (lipid-requiring) lifestyle and a remarkable repertoire of carbohydrate uptake and utilization systems. Clinical isolates of C. kroppenstedtii have been obtained almost exclusively from female patients and mainly from breast abscesses and cases of granulomatous mastitis. However, the role of C. kroppenstedtii in breast pathologies remains unclear. This review provides a comprehensive overview of the taxonomy, microbiology, and microbiological identification of C. kroppenstedtii, including polyphasic phenotypic approaches, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the use of 16S rRNA gene sequencing. A clinical review presents reported cases, various antimicrobial treatments, antibiotic susceptibility assays, and antibiotic resistance genes detected during genome sequencing. C. kroppenstedtii must be considered a potential opportunistic human pathogen and should be identified accurately in clinical laboratories.

show abstract

Draft Genome Sequences of Corynebacterium kroppenstedtii CNM633/14 and CNM632/14, Multidrug-Resistant and Antibiotic-Sensitive Isolates from Nodules of Granulomatous Mastitis Patients

Cited by 8 publications

References 15 publications

Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis

Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis

Taxonomy, Physiology, and Natural Products of Actinobacteria

A microbiological and clinical review on Corynebacterium kroppenstedtii

Contact Info

Product

Resources

About