Draft Genome Sequences of 37 Salmonella enterica Strains Isolated from Poultry Sources in Nigeria

Useh, N. M.; Ngbede, Emmanuel O.; Akange, Nguavese; Thomas, M. G.; Foley, Andrew; Keena, Mitchel Chan; Nelson, Eric; Christopher‐Hennings, Jane; Tomita, Masaru; Suzuki, Haruo; Scaria, Joy

doi:10.1128/genomea.00315-16

Cited by 7 publications

(11 citation statements)

References 7 publications

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“…Also S. Typhimurium, which is commonly associated with poultry [54], was not observed in this study. This confirms observations by Fagbamila et al, (2017) and Useh et al, (2016) that these two serotypes play marginal role in the poultry industry in Nigeria. S. Kentucky was the most commonly observed serotype.…”

Section: Plos Onesupporting

confidence: 91%

“…A high number of Salmonella serotypes were observed in the farms investigated suggesting either a wide diversity of sources for introduction of Salmonella into the farms, or that common sources (such as contaminated feed) can contain different serotypes over time. Reports from other countries have also showed diversity in serotypes of non- typhoidal Salmonella in poultry farms [ 41 , 42 , 50 – 52 ]. Notably, S .…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

et al. 2020

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Salmonella is an important human pathogen and poultry products constitute an important source of human infections. This study investigated prevalence; identified serotypes based on whole genome sequence, described spatial distribution of Salmonella serotypes and predicted risk factors that could influence the prevalence of Salmonella infection in commercial poultry farms in Nigeria. A cross sectional approach was employed to collect 558 pooled shoe socks and dust samples from 165 commercial poultry farms in North West Nigeria. On-farm visitation questionnaires were administered to obtain information on farm management practices in order to assess risk factors for Salmonella prevalence. Salmonella was identified by culture, biotyping, serology and polymerase chain reaction (PCR). PCR confirmed isolates were paired-end Illumina-sequenced. Following de novo genome assembly, draft genomes were used to obtain serotypes by SeqSero2 and SISTR pipeline and sequence types by SISTR and Enterobase. Risk factor analysis was performed using the logit model. A farm prevalence of 47.9% (CI 95 [40.3-55.5]) for Salmonella was observed, with a sample level prevalence of 15.9% (CI 95 [12.9-18.9]). Twenty-three different serotypes were identified, with S. Kentucky and S. Isangi as the most prevalent (32.9% and 11%). Serotypes showed some geographic variation. Salmonella detection was strongly associated with disposal of poultry waste and with presence of other livestock on the farm. Salmonella was commonly detected on commercial poultry farms in North West Nigeria and S. Kentucky was found to be ubiquitous in the farms.

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Section: Plos Onesupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

et al. 2020

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“…While there is paucity of information on the pathogenicity of some of the Salmonella serotypes identified in the present study, the availability of draft genomes of these serotypes have earlier been reported. 37 …”

Section: Discussionmentioning

confidence: 99%

Motile Salmonella serotypes causing high mortality in poultry farms in three South‐Western States of Nigeria

Mshelbwala

Ibrahim

Saidu

et al. 2017

Veterinary Record Open

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This study was carried out to identify the Salmonella serotypes causing high mortality in chickens in Lagos, Ogun and Oyo states, Nigeria. Chickens presented for postmortem examination during disease outbreaks that were characterised by high mortality (40 per cent to 80 per cent) in poultry farms in the study area were examined from January to December, 2013. Samples of the lungs, heart, liver, spleen, kidneys, proventriculus, intestine and caecum were collected from suspected cases of salmonellosis, for bacterial culture and identification. Salmonella isolates were confirmed using PCR and serotyped using the Kauffman-White scheme. Twenty-six day-old pullets were raised to two weeks and inoculated orally with 0.2 mL of 1×108 colony forming units of Salmonella Zega identified in the present study to determine their pathogenicity, while another 26 served as control. The Salmonella serotypes were S Zega (n=13; 35.14 per cent), Salmonella Kentucky (n=9; 24.32 per cent), Salmonella Herston (n=6; 16.22 per cent), Salmonella Nima (n=4; 10.81 per cent), Salmonella Telelkebir (n=3; 8.11 per cent), Salmonella Colindale (n=1; 2.70 per cent) and Salmonella Tshiongwe (n=1; 2.70 per cent). Clinical signs in both natural and experimental infections were acute (70 per cent) and chronic (30 per cent), and included weakness, anorexia, yellowish diarrhoea, pasted vents, somnolescence and mortality, while gross lesions showed marked pulmonary congestion and oedema, necrotic foci in the myocardium; the liver, spleen and kidneys were markedly enlarged and had subcapsular multifocal necrosis. There were catarrhal proventriculitis and enteritis, and haemorrhagic typhlitis. While most of the serotypes identified in the present study have been isolated from poultry sources from commercial farms in Nigeria, to the best of the authors' knowledge, they have not been previously reported to cause high mortality in chickens in the study area.

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“…Stocked culture of Salmonella enterica serovar Zega originally isolated from commercial layers in South-western Nigeria, identified at the Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA [9] and maintained at the bacterial bank, Bacterial Research Department, National Veterinary Research Institute, Vom, Nigeria was used in this study.…”

Section: Bacterial Isolate Used In the Studymentioning

confidence: 99%

“…Salmonella enterica serovar Zega, one of the over 3000 identified strains of salmonella were first isolated from dead ducklings in the Belgian Congo (now Democratic Republic of Congo) in 1952 [8]. Recently, it was listed amongst 37 Salmonella enterica strains isolated from commercial layer flocks in Southwest Nigeria [9] [10]. This suggests that the agent may be pathogenic for avian species.…”

Section: Introductionmentioning

confidence: 99%

Haematological and Serum Biochemistry Profile of Cockerels Experimentally Infected with <i>Salmonella enterica</i> Serovar Zega

Emennaa¹,

Eze²,

Chah³

et al. 2019

OJVM

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Salmonellosis is a serious medical and veterinary problem worldwide and causes great concern in the food and livestock industries, especially the poultry industry which occupies a prominent position in the provision of animal protein and accounts for about 25% of local meat production in Nigeria particularly and is identified as a disease of major economic importance causing low performance in poultry production. The study was carried out at the experimental animal farm, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria. One hundred (100) five-week old chickens obtained from the Poultry division of National Veterinary Research Institute, Vom, were used for the experiment. The birds were randomly assigned to 4 groups of 25 birds per group (A, B, C, D). Each bird in all the groups received 0.5 ml of PBS containing 1 × 108 cfu/ml of Salmonella enterica serovar Zega as follows: Group A was infected with Salmonella Zega intra-nasally (IN). Group B was infected with Salmonella Zega intra-peritonealy (IP). Group C was infected with Salmonella Zega orally (OR). Group D was the Uninfected control (CT). There was a significant change (p < 0.05) in the mean corpuscular volume (MCV) post-infection in all infected groups, however no statistical significant changes (p > 0.05) in the mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) in the infected groups compared across the different days post infection. There was significant increase (p < 0.05) in the mean aspartate transferase (AST) and mean alanine transferase (ALT) after infection in all the infected groups between How to cite this paper: Emennaa, P.E.,

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Draft Genome Sequences of 37 Salmonella enterica Strains Isolated from Poultry Sources in Nigeria

Cited by 7 publications

References 7 publications

Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

Motile Salmonella serotypes causing high mortality in poultry farms in three South‐Western States of Nigeria

Haematological and Serum Biochemistry Profile of Cockerels Experimentally Infected with <i>Salmonella enterica</i> Serovar Zega

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Draft Genome Sequences of 37 Salmonella enterica Strains Isolated from Poultry Sources in Nigeria

Cited by 7 publications

References 7 publications

Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria

Motile Salmonella serotypes causing high mortality in poultry farms in three South‐Western States of Nigeria

Haematological and Serum Biochemistry Profile of Cockerels Experimentally Infected with &lt;i&gt;Salmonella enterica&lt;/i&gt; Serovar Zega

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Haematological and Serum Biochemistry Profile of Cockerels Experimentally Infected with <i>Salmonella enterica</i> Serovar Zega