Draft Genome Sequences of 17 French Clostridium botulinum Group III Strains

Woudstra, Cédric; Maréchal, Caroline Le; Souillard, Rozenn; Bayon-Auboyer, Marie-Hélène; Mermoud, Isabelle; Desoutter, Denise; Fach, Patrick

doi:10.1128/genomea.01105-15

Cited by 5 publications

(6 citation statements)

References 5 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While various aspects of the physiology and genomics of C. botulinum Group III have been studied in some detail (Eklund and Dowell, 1987; Woudstra et al, 2015), relatively little is known about the properties of their spores or their spore germination characteristics. Based on analysis of the encoded GRs, it is anticipated that these spores will also respond to amino acid germinants.…”

Section: Resultsmentioning

confidence: 99%

“…Strains form a single neurotoxin of type B, E, or F. C. botulinum Group II is a saccharolytic and psychrotrophic bacterium that forms spores of moderate heat resistance (Peck, 2009; Stringer et al, 2013). Strains of C. botulinum Group III form a single neurotoxin of type C or type D or more commonly a hybrid neurotoxin that comprises elements of each (type C/D or D/C), and are responsible for botulism in a wide range of animal species (Lindstrom et al, 2004; Sharpe et al, 2008; Woudstra et al, 2015). C. botulinum Group III is a saccharolytic and mesophilic bacterium that closely resembles C. novyi and C. haemolyticum (Skarin and Segerman, 2014) and forms spores of high heat resistance (Sasaki et al, 2001; Woudstra et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diversity of the Germination Apparatus in Clostridium botulinum Groups I, II, III, and IV

et al. 2016

View full text Add to dashboard Cite

Clostridium botulinum is a highly dangerous pathogen that forms very resistant endospores that are ubiquitous in the environment, and which, under favorable conditions germinate to produce vegetative cells that multiply and form the exceptionally potent botulinum neurotoxin. To improve the control of botulinum neurotoxin-forming clostridia, it is important to understand the mechanisms involved in spore germination. Here we present models for spore germination in C. botulinum based on comparative genomics analyses, with C. botulinum Groups I and III sharing similar pathways, which differ from those proposed for C. botulinum Groups II and IV. All spores germinate in response to amino acids interacting with a germinant receptor, with four types of germinant receptor identified [encoded by various combinations of gerA, gerB, and gerC genes (gerX)]. There are three gene clusters with an ABC-like configuration; ABC [gerX1], ABABCB [gerX2] and ACxBBB [gerX4], and a single CA-B [gerX3] gene cluster. Subtypes have been identified for most germinant receptor types, and the individual GerX subunits of each cluster show similar grouping in phylogenetic trees. C. botulinum Group I contained the largest variety of gerX subtypes, with three gerX1, three gerX2, and one gerX3 subtypes, while C. botulinum Group III contained two gerX1 types and one gerX4. C. botulinum Groups II and IV contained a single germinant receptor, gerX3 and gerX1, respectively. It is likely that all four C. botulinum Groups include a SpoVA channel involved in dipicolinic acid release. The cortex-lytic enzymes present in C. botulinum Groups I and III appear to be CwlJ and SleB, while in C. botulinum Groups II and IV, SleC appears to be important.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of the Germination Apparatus in Clostridium botulinum Groups I, II, III, and IV

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Whole genome sequencing was performed using an Illumina MiSeq platform (Illumina) according to the manufacturer’s instructions. Three MiSeq runs were carried out, two with paired-end 150-nucleotide (nt) reads on MiSeq V2 and V2 microchemistry, another with paired-end 300-nt reads on V3 chemistry ( Woudstra et al, 2015b ).…”

Section: Methodsmentioning

confidence: 99%

“…De novo assembly was performed as previously described ( Woudstra et al, 2015b ). Raw reads were trimmed (minimum length 35 bp, quality score 0.03) and assembled with the CLC Genomics Workbench version 7.5.1 (Qiagen, Aarhus, Denmark).…”

Section: Methodsmentioning

confidence: 99%

“…Given this situation, our objective was to investigate C. botulinum group III genome sequences from France to complete the genetic picture of European C. botulinum group III strains and to find new potential genetic targets for typing. The genome sequences of 17 C. botulinum group III strains were analyzed: 14 type C/D strains from chickens, ducks, guinea fowl, and turkeys, plus three type D/C strains from cattle, all from France except for one type D/C strain which was from New Caledonia ( Woudstra et al, 2015b ). The sequences produced were processed and assembled either with a mapping or de novo process before being investigated for their genetic diversity using bio-informatic tools.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New Insights into the Genetic Diversity of Clostridium botulinum Group III through Extensive Genome Exploration

et al. 2016

Self Cite

View full text Add to dashboard Cite

Animal botulism is caused by group III Clostridium botulinum strains producing type C and D toxins, or their chimeric forms C/D and D/C. Animal botulism is considered an emerging disease in Europe, notably in poultry production. Before our study, 14 genomes from different countries were available in the public database, but none were from France. In order to investigate the genetic relationship of French strains with different geographical areas and find new potential typing targets, 17 strains of C. botulinum group III were sequenced (16 from France and one from New Caledonia). Fourteen were type C/D strains isolated from chickens, ducks, guinea fowl and turkeys and three were type D/C strains isolated from cattle. The New Caledonian strain was a type D/C strain. Whole genome sequence analysis showed the French strains to be closely related to European strains from C. botulinum group III lineages Ia and Ib. The investigation of CRISPR sequences as genetic targets for differentiating strains in group III proved to be irrelevant for type C/D due to a deficient CRISPR/Cas mechanism, but not for type D/C. Conversely, the extrachromosomal elements of type C/D strains could be used to generate a genetic ID card. The highest level of discrimination was achieved with SNP core phylogeny, which allowed differentiation up to strain level and provide the most relevant information for genetic epidemiology studies and discrimination.

show abstract

Species-specific gene duplication in Clostridia produces variations of cholesterol-dependent cytolysin with different cytotoxicity

Hosoya

Miyashita

Karatsu

et al. 2022

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Draft Genome Sequences of 17 French Clostridium botulinum Group III Strains

Cited by 5 publications

References 5 publications

Diversity of the Germination Apparatus in Clostridium botulinum Groups I, II, III, and IV

Diversity of the Germination Apparatus in Clostridium botulinum Groups I, II, III, and IV

New Insights into the Genetic Diversity of Clostridium botulinum Group III through Extensive Genome Exploration

Species-specific gene duplication in Clostridia produces variations of cholesterol-dependent cytolysin with different cytotoxicity

Contact Info

Product

Resources

About