Draft Genome Sequences of 10 Bacteria from the Marine
            <i>Pseudoalteromonas</i>
            Group

Alker, Amanda T.; Gode, Bhumika S.; Aspiras, Alpher E.; Jones, Jeffrey E.; Michael, Sama R.; Aguilar, David; Cain, Audrea D.; Candib, Alec M.; Cizmic, Julian M.; Clark, Elise A.; Cozzo, Alyssa C.; Figueroa, Laura E.; Garcia, Peter A.; Heaney, Casey M.; Levy, Alexandra T.; Macknight, Luke; McCarthy, A; McNamara, J. P.; Nguyen, Kelvin A.; Rollin, Kendall N.; Salcedo, Gabriella Y.; Showalter, Julia A.; Sue, Andrew; Zamro, Tony R.; Dunbar, Tiffany L.; Malter, Kyle E.; Shikuma, Nicholas J.

doi:10.1128/mra.00404-21

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…In our previous study, we isolated a Pseudoalteromonas strain FDHY-MZ2 (Shi et al, 2022), which showed significantly lower algicidal activity against H. akashiwo compared to FDHY-MQ5. These differences could be associated with the significant differences in the genome specific species of Pseudoalteromonas (Alker et al, 2021). Simultaneously, it is plausible that microbial competition, coupled with its impacts on the co-culture of algicidal bacteria and phytoplankton, might contribute to the observed differential species representation.…”

Section: Discussionmentioning

confidence: 99%

Decoding the influence of bacterial community structure and algicidal bacteria in a Karenia longicanalis bloom event

Zhang,

Zhou,

Tang

et al. 2023

Front. Mar. Sci.

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IntroductionHarmful algal blooms (HABs) have been increasing in frequency and expanding their ranges on coastlines worldwide in recent decades. Algicidal bacteria play a pivotal role in eliminating HABs, yet the characteristics of bacterial communities and their algicidal activity during a Karenia longicanalis bloom remain poorly understood.MethodsIn this study, we investigated bacterial communities using 16S rRNA sequencing during a K. longicanalis bloom to identify bacteria with high algicidal activity that could be isolated. Five sampling sites in Tongxin Bay, located in Lianjiang County, China, including TX1 to TX5, were selected based on the concentration of K. longicanalis cells.ResultsOur 16S rRNA sequencing results revealed that the TX4 site was enriched with genera known to contain algicidal bacteria, such as Pseudoalteromonas and Alteromonas, which are members of the Gammaproteobacteria class, while Sulfitobacter, a member of the Alphaproteobacteria class, was enriched in the TX5 site. Among the 100 cultivable bacteria isolated from the 5 sampling sites, 6 exhibited an algicidal rate of over 80%, with FDHY-MQ5, isolated from the TX4 site, exhibiting an algicidal rate of approximately 100% against Karenia mikimotoi after 48 hours of challenge with 2% (v/v) bacterial volume (OD600=4.5) concentration. Our 16S rRNA sequencing result showed FDHY-MQ5 was a member of the Pseudoalteromonas genus, and this bacterium also demonstrated high algicidal activity against Heterosigma akashiwo and Alexandrium tamarense.DiscussionOur findings shed light on the changes in bacterial community structure and the algicidal behavior of bacteria towards algae during a K. longicanalis bloom, providing a research basis for a better understanding of HAB management.

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Section: Discussionmentioning

confidence: 99%

Decoding the influence of bacterial community structure and algicidal bacteria in a Karenia longicanalis bloom event

Zhang,

Zhou,

Tang

et al. 2023

Front. Mar. Sci.

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Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech

et al. 2023

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Sponges are the richest source of bioactive organic small molecules, referred to as natural products, in the marine environment. It is well established that laboratory culturing-resistant symbiotic bacteria residing within the eukaryotic sponge host matrix often synthesize the natural products that are detected in the sponge tissue extracts. However, the contributions of the culturing-amenable commensal bacteria that are also associated with the sponge host to the overall metabolome of the sponge holobiont are not well defined. In this study, we cultured a large library of bacteria from three marine sponges commonly found in the Florida Keys. Metabolomes of isolated bacterial strains and that of the sponge holobiont were compared using mass spectrometry to reveal minimal metabolomic overlap between commensal bacteria and the sponge hosts. We also find that the phylogenetic overlap between cultured commensal bacteria and that of the sponge microbiome is minimal. Despite these observations, the commensal bacteria were found to be a rich resource for novel natural product discovery. Mass spectrometry-based metabolomics provided structural insights into these cryptic natural products. Pedagogic innovation in the form of laboratory curricula development is described which provided undergraduate students with hands-on instruction in microbiology and natural product discovery using metabolomic data mining strategies.

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Draft Genome Sequences of 10 Bacteria from the Marine Pseudoalteromonas Group

Cited by 2 publications

References 19 publications

Decoding the influence of bacterial community structure and algicidal bacteria in a Karenia longicanalis bloom event

Decoding the influence of bacterial community structure and algicidal bacteria in a Karenia longicanalis bloom event

Limited Metabolomic Overlap between Commensal Bacteria and Marine Sponge Holobionts Revealed by Large Scale Culturing and Mass Spectrometry-Based Metabolomics: An Undergraduate Laboratory Pedagogical Effort at Georgia Tech

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