Draft Genome Sequence of Thermotoga maritima A7A Reconstructed from Metagenomic Sequencing Analysis of a Hydrocarbon Reservoir in the Bass Strait, Australia

Sutcliffe, Brodie; Midgley, David J.; Rosewarne, Carly P.; Greenfield, Paul; Li, Dongmei

doi:10.1128/genomea.00688-13

Cited by 4 publications

(5 citation statements)

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“…2812B; Figure 5) suggests that the Thermotoga population has entered the Tuna reservoir recently from a marine environment. The temperature of the reservoir is reported to be 105 1C (Sutcliffe et al, 2013), which is considerably higher than the proposed 80-90 1C threshold temperature for sustaining life in oil reservoirs (Stetter and Huber, 1999;Wilhelms et al, 2001), suggesting that Thermotoga sp. A7A may be inhabiting the petroleum pipelines, where the temperature is lower.…”

Section: Resultsmentioning

confidence: 99%

“…populations with similar properties To investigate whether the observed patterns of extensive recombination and low within-population diversity can be generalized for other TM-group populations, we searched for publicly available metagenomes that harbor TM-group bacteria, using as a criterion 490% identity to Thermotoga genus genes. We identified three such sample sites (Supplementary Table S7): San Juan basin coal bed production water , a 'continental hot spring' fed from a subterranean reservoir (Great Boiling Spring (GBS), Nevada) (Costa et al, 2009) and the off-shore Tuna oil reservoir in the Bass Strait, Gippsland Basin, Australia (Sutcliffe et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…GBS) and the already assembled draft genome of Thermotoga sp. A7A from the Tuna oil reservoir metagenome (Sutcliffe et al, 2013) allowed us to repeat the genome-wide phylogenetic analyses with 9 TMgroup genomes. On the 'core SNPs' network, Thermotoga sp.…”

Section: Resultsmentioning

confidence: 99%

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Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments

et al. 2014

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Oil reservoirs represent a nutrient-rich ecological niche of the deep biosphere. Although most oil reservoirs are occupied by microbial populations, when and how the microbes colonized these environments remains unanswered. To address this question, we compared 11 genomes of Thermotoga maritima-like hyperthermophilic bacteria from two environment types: subsurface oil reservoirs in the North Sea and Japan, and marine sites located in the Kuril Islands, Italy and the Azores. We complemented our genomes with Thermotoga DNA from publicly available subsurface metagenomes from North America and Australia. Our analysis revealed complex non-bifurcating evolutionary history of the isolates' genomes, suggesting high amounts of gene flow across all sampled locations, a conjecture supported by numerous recombination events. Genomes from the same type of environment tend to be more similar, and have exchanged more genes with each other than with geographically close isolates from different types of environments. Hence, Thermotoga populations of oil reservoirs do not appear isolated, a requirement of the 'burial and isolation' hypothesis, under which reservoir bacteria are descendants of the isolated communities buried with sediments that over time became oil reservoirs. Instead, our analysis supports a more complex view, where bacteria from subsurface and marine populations have been continuously migrating into the oil reservoirs and influencing their genetic composition. The Thermotoga spp. in the oil reservoirs in the North Sea and Japan probably entered the reservoirs shortly after they were formed. An Australian oil reservoir, on the other hand, was likely colonized very recently, perhaps during human reservoir development.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments

et al. 2014

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“…The resultant DNA was sequenced using 100-bp paired-end Illumina HiSeq at the University of Western Sydney. A genome was separated from the metagenome and assembled using the procedure described by Sutcliffe et al (2013) (1). This approach identified three abundant organisms in the metagenome sample: Thermotoga maritima A7A (1), Desulfonauticus sp.…”

Section: Genome Announcementmentioning

confidence: 99%

“…The other two species that occur in the Tuna oil field at the A7A oil well, Thermotoga maritima A7A (1) and Desulfonauticus sp. A7A, will be described in separate genome announcements and a comparative analysis will be undertaken to understand how these three species interact in the subsurface.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Thermoanaerobacter sp. Strain A7A, Reconstructed from a Metagenome Obtained from a High-Temperature Hydrocarbon Reservoir in the Bass Strait, Australia

Greenfield

Rosewarne

et al. 2013

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T hermotoga

Nesbø¹,

Farrell²,

Zhaxybayeva³

et al. 2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Ther.mo.to'ga. Gr. fem. n. therme heat: L. fem. n. toga Roman outer garment: N.L. fem. n. Thermotoga the hot outer garment. Thermotogota / Thermotogae / Thermotogales / Thermotogaceae / Thermotoga The genus Thermotoga comprises sheathed, hyperthermophilic, anaerobic, fermentative, and hydrogen‐producing bacteria. These bacteria reduce thiosulfate and sulfur to sulfide. Thermotoga spp. are members of the phylum Thermotogota , class Thermotogae , order Thermotogales , family Thermotogaceae . Known habitats are geothermal heated marine sediments, shallow submarine thermal vents, hot springs, and oil reservoirs. Genome sizes of closed genomes are in the range 1.75–1.88 Mb, and GC content is 46.1–47.1%. Thermotoga spp. display high levels of homologous recombination and lateral gene transfer with both closely related and distantly related lineages. Phylogenetic analyses of 16S rRNA genes assign two recently described species, T. profunda and T. caldifontis, to the recently proposed genus Pseudothermotoga . Hence, we propose an emended description of the genus Thermotoga , which consists of four validly named species: T. maritima , T. neapolitana , T. naphthophila , and T. petrophila . DNA G + C content (mol%) : 46.1–47.1 (genome analysis). Type species : Thermotoga maritima Stetter and Huber 1986, 575 VP (Effective publication: Stetter and Huber in Huber, Langworthy, König, Thomm, Woese, Sleytr et al. 1986)

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Draft Genome Sequence of Thermotoga maritima A7A Reconstructed from Metagenomic Sequencing Analysis of a Hydrocarbon Reservoir in the Bass Strait, Australia

Cited by 4 publications

References 10 publications

Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments

Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments

Draft Genome Sequence of Thermoanaerobacter sp. Strain A7A, Reconstructed from a Metagenome Obtained from a High-Temperature Hydrocarbon Reservoir in the Bass Strait, Australia

T hermotoga

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