Draft Genome Sequence of the Fungus
            <i>Penicillium brasilianum</i>
            MG11

Horn, Fabian; Linde, Jörg; Mattern, Derek J.; Walther, Grit; Guthke, Reinhard; Brakhage, Axel A.; Valiante, Vito

doi:10.1128/genomea.00724-15

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…The final two viruses identified within the Partitiviridae branch of the phylogenetic tree (Fig 2) are Penicillium brasilianum partitivirus 1 (PbPV1) (PRJEB7514; [22]) and Delitschia confertaspora partitivirus 1 (DcPV1) (PRJNA250740; unpublished). Both viruses, the first to be described in these fungal species, phylogenetically group with Alternaria alternata partitivirus 1 (AaPV1) [54] and Botryosphaeria dothidea partitivirus 1 (BdPV1) [55], a distinct clade within the Partitiviridae that is related to, but separate from, the genus Gammapartitivirus (Fig 2).…”

Section: Resultsmentioning

confidence: 99%

Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes

et al. 2019

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The distribution and diversity of RNA viruses in fungi is incompletely understood due to the often cryptic nature of mycoviral infections and the focused study of primarily pathogenic and/or economically important fungi. As most viruses that are known to infect fungi possess either single-stranded or double-stranded RNA genomes, transcriptomic data provides the opportunity to query for viruses in diverse fungal samples without any a priori knowledge of virus infection. Here we describe a systematic survey of all transcriptomic datasets from fungi belonging to the subphylum Pezizomycotina. Using a simple but effective computational pipeline that uses reads discarded during normal RNA-seq analyses, followed by identification of a viral RNA-dependent RNA polymerase (RdRP) motif in de novo assembled contigs, 59 viruses from 44 different fungi were identified. Among the viruses identified, 88% were determined to be new species and 68% are, to our knowledge, the first virus described from the fungal species. Comprehensive analyses of both nucleotide and inferred protein sequences characterize the phylogenetic relationships between these viruses and the known set of mycoviral sequences and support the classification of up to four new families and two new genera. Thus the results provide a deeper understanding of the scope of mycoviral diversity while also increasing the distribution of fungal hosts. Further, this study demonstrates the suitability of analyzing RNA-seq data to facilitate rapid discovery of new viruses.

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Section: Resultsmentioning

confidence: 99%

Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes

et al. 2019

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“…After we obtained the genome sequence of P. brasilianum NBRC 6234, the genome sequence of another strain, P. brasilianum MG11, was published . Interestingly, although these two strains belong to the same species, P. brasilianum MG11 reportedly produces austinol and its derivatives (Figure B), and paraherquonin ( 1 ) and the other meroterpenoids shown in Figure A have never been isolated from the fungus.…”

Section: Resultsmentioning

confidence: 99%

Discovery of Key Dioxygenases that Diverged the Paraherquonin and Acetoxydehydroaustin Pathways in Penicillium brasilianum

et al. 2016

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Paraherquonin (1), a fungal meroterpenoid produced by Penicillium brasilianum NBRC 6234, possesses a unique, highly congested hexacyclic molecular architecture. Here we identified the biosynthetic gene cluster of 1 (the prh cluster) and elucidated the pathway up to berkeleydione (2), which serves as the key intermediate for the biosynthesis of 1 as well as many other meroterpenoids. Interestingly, the nonheme iron and α-ketoglutarate-dependent dioxygenase PrhA constructs the cycloheptadiene moiety to afford 2 from preaustinoid A1 (6), probably via the homoallyl-homoallyl radical rearrangement. Additionally, another fungal strain, P. brasilianum MG11, which produces acetoxydehydroaustin instead of 1, was found to have a gene cluster nearly identical to the prh cluster. The dioxygenase encoded by the cluster shares 92% sequence identity with PrhA, and also accepts 6 but produces preaustinoid A3 (17) with a spiro-lactone system, generating a diverging point for the two different meroterpenoid pathways in the same species.

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“…The final two viruses identified within the Partitiviridae branch of the phylogenetic tree ( Fig 2) are Penicillium brasilianum partitivirus 1 (PbPV1) (PRJEB7514; [42] ) and Delitschia confertaspora partitivirus 1 (DcPV1) (PRJNA250740; unpublished). Both viruses, the first to be described in these fungal species, phylogenetically group with Alternaria alternata partitivirus 1 (AaPV1) [43] and Botryosphaeria dothidea partitivirus 1 (BdPV1) [44] , a distinct clade within the Partitiviridae that is related to, but separate from, the genus Gammapartitivirus (Fig 2).…”

Section: New Genus: Zetapartitivrusmentioning

confidence: 99%

Discovery of new mycoviral genomes within publicly available fungal transcriptomic datasets

Gilbert

Holcomb

Allscheid

et al. 2019

Preprint

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The distribution and diversity of RNA viruses in fungi is incompletely understood due to the often cryptic nature of mycoviral infections and the focused study of primarily pathogenic and/or economically important fungi. As most viruses that are known to infect fungi possess either single-stranded or double-stranded RNA genomes, transcriptomic data provides the opportunity to query for viruses in diverse fungal samples without any a priori knowledge of virus infection.Here we describe a systematic survey of all transcriptomic datasets from fungi belonging to the subphylum Pezizomycotina. Using a simple but effective computational pipeline that uses reads discarded during normal RNA-seq analyses, followed by identification of a viral RNA-dependent RNA polymerase (RdRP) motif in de novo assembled contigs, 59 viruses from 44 different fungi were identified. Among the viruses identified, 89% were determined to be new species and 68% are, to our knowledge, the first virus described from the fungal species. Comprehensive analyses of both nucleotide and inferred protein sequences characterize the phylogenetic relationships between these viruses and the known set of mycoviral sequences and support the classification of up to four new families and two new genera. Thus the results provide a deeper understanding of the scope of mycoviral diversity while also increasing the distribution of fungal hosts. Further, this study demonstrates the suitability of analyzing RNA-seq data to facilitate rapid discovery of new viruses.

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Draft Genome Sequence of the Fungus Penicillium brasilianum MG11

Cited by 11 publications

References 23 publications

Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes

Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes

Discovery of Key Dioxygenases that Diverged the Paraherquonin and Acetoxydehydroaustin Pathways in Penicillium brasilianum

Discovery of new mycoviral genomes within publicly available fungal transcriptomic datasets

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