Draft Genome Sequence of the Marine-Derived, Anticancer Compound-Producing Bacterium
            <i>Streptomyces</i>
            sp. Strain GMY01

Widada, Jaka; Damayanti, Ema; Herdini, Camelia; Wijayanti, Nastiti; Hosoyama, Akira; Yamazoe, Atsushi; Suzuki-Minakuchi, Chiho; Hariwiyanto, Bambang; Miceli, Sofia; Dinoto, Achmad; Mustofa, .; Nojiri, Hideaki

doi:10.1128/mra.01366-20

Cited by 1 publication

(1 citation statement)

References 13 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GMY01 was performed using a combined shotgun sequencing method with the 454 GS FLX Titanium system (Roche) and paired-end (PE) sequencing using the HiSeq 1000 platform (Illumina). The NGS of GMY01 was conducted at Biological Resource Center, NITE (NBRC), Japan, as reported in the previous study [32]. The assembly of the whole-genome sequence was analyzed using Type (Strain) Genome Server (TYGS), a free bioinformatics platform available at https://tygs.dsmz.de (accessed on 18 June 2023), for a whole-genome-based taxonomic analysis, including digital DNA-DNA hybridization (dDDH), G+C content comparison, and phylogeny based on whole-genome sequence [33].…”

Section: Whole-genome and Taxonogenomic Analysismentioning

confidence: 99%

Marine-Derived Streptomyces sennicomposti GMY01 with Anti-Plasmodial and Anticancer Activities: Genome Analysis, In Vitro Bioassay, Metabolite Profiling, and Molecular Docking

Widada

Damayanti²,

Mustofa

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

To discover novel antimalarial and anticancer compounds, we carried out a genome analysis, bioassay, metabolite profiling, and molecular docking of marine sediment actinobacteria strain GMY01. The whole-genome sequence analysis revealed that Streptomyces sp. GMY01 (7.9 Mbp) is most similar to Streptomyces sennicomposti strain RCPT1-4T with an average nucleotide identity (ANI) and ANI based on BLAST+ (ANIb) values of 98.09 and 97.33% (>95%). An in vitro bioassay of the GMY01 bioactive on Plasmodium falciparum FCR3, cervical carcinoma of HeLa cell and lung carcinoma of HTB cells exhibited moderate activity (IC50 value of 46.06; 27.31 and 33.75 µg/mL) with low toxicity on Vero cells as a normal cell (IC50 value of 823.3 µg/mL). Metabolite profiling by LC-MS/MS analysis revealed that the active fraction of GMY01 contained carbohydrate-based compounds, C17H29NO14 (471.15880 Da) as a major compound (97.50%) and mannotriose (C18H32O16; 504.16903 Da, 1.96%) as a minor compound. Molecular docking analysis showed that mannotriose has a binding affinity on glutathione reductase (GR) and glutathione-S-transferase (GST) of P. falciparum and on autophagy proteins (mTORC1 and mTORC2) of cancer cells. Streptomyces sennicomposti GMY01 is a potential bacterium producing carbohydrate-based bioactive compounds with anti-plasmodial and anticancer activities and with low toxicity to normal cells.

show abstract

Section: Whole-genome and Taxonogenomic Analysismentioning

confidence: 99%

Marine-Derived Streptomyces sennicomposti GMY01 with Anti-Plasmodial and Anticancer Activities: Genome Analysis, In Vitro Bioassay, Metabolite Profiling, and Molecular Docking

Widada

Damayanti²,

Mustofa

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

Draft Genome Sequence of the Marine-Derived, Anticancer Compound-Producing Bacterium Streptomyces sp. Strain GMY01

Abstract: The marine Streptomyces sp. strain GMY01 was isolated from Indonesian marine sediment. Genome mining analysis revealed that GMY01 has 28 biosynthetic gene clusters, dominated by genes encoding nonribosomal peptide synthetase and polyketide synthase.

Cited by 1 publication

References 13 publications

Marine-Derived Streptomyces sennicomposti GMY01 with Anti-Plasmodial and Anticancer Activities: Genome Analysis, In Vitro Bioassay, Metabolite Profiling, and Molecular Docking

Marine-Derived Streptomyces sennicomposti GMY01 with Anti-Plasmodial and Anticancer Activities: Genome Analysis, In Vitro Bioassay, Metabolite Profiling, and Molecular Docking

Contact Info

Product

Resources

About