Draft genome sequence of the marine Rhodobacteraceae strain O3.65, cultivated from oil-polluted seawater of the Deepwater Horizon oil spill

Abstract

The marine alphaproteobacterium strain O3.65 was isolated from an enrichment culture of surface seawater contaminated with weathered oil (slicks) from the Deepwater Horizon (DWH) oil spill and belongs to the ubiquitous, diverse and ecological relevant Roseobacter group within the Rhodobacteraceae. Here, we present a preliminary set of physiological features of strain O3.65 and a description and annotation of its draft genome sequence. Based on our data we suggest potential ecological roles of the isolate in the degradation of crude oil within the network of the oil-enriched microbial community. The draft genome comprises 4,852,484 bp with 4,591 protein-coding genes and 63 RNA genes. Strain O3.65 utilizes pentoses, hexoses, disaccharides and amino acids as carbon and energy source and is able to grow on several hydroxylated and substituted aromatic compounds. Based on 16S rRNA gene comparison the closest described and validated strain is Phaeobacter inhibens DSM 17395, however, strain O3.65 is lacking several phenotypic and genomic characteristics specific for the genus Phaeobacter. Phylogenomic analyses based on the whole genome support extensive genetic exchange of strain O3.65 with members of the genus Ruegeria, potentially by using the secretion system type IV. Our physiological observations are consistent with the genomic and phylogenomic analyses and support that strain O3.65 is a novel species of a new genus within the Rhodobacteraceae.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-016-0201-7) contains supplementary material, which is available to authorized users.

Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico

“…In fact, Klotz et al isolated a new Roseobacter strain, Tritonibacter horizontis , from oil contaminated surface water collected in the Gulf of Mexico during the DWH discharge (Klotz et al, 2018). The strain demonstrated the ability to utilize hydroxylated and substituted aromatic compounds but cannot grow on alkanes (Giebel et al, 2016). Thompson et al (2017) also found that members of this lineage were enriched by oil, especially in the presence of phytoplankton.…”

Fine-scale succession patterns and assembly mechanisms of bacterial community of Litopenaeus vannamei larvae across the developmental cycle

“…Therefore, the family Rhodobacteraceae is likely a core group of intestinal microbiota of L. vannamei. Rhodobacteraceae are heterotrophic bacteria with extremely high diversity and versatility in organic matter degradation, and widely distributed across various marine ecosystems [68][69][70]. Most Rhodobacteraceae taxa are able to synthesize vitamin B 12 , which is a dietary essential for shrimps [71].…”

Enrichment and key features of a robust and consistent indigenous marine-cognate microbial consortium growing on oily bilge wastewaters

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