Draft Genome Sequence of the Thermophilic Acetogen Moorella humiferrea DSM 23265

Abstract

Moorella humiferrea is an endospore-forming, anaerobic, and thermophilic bacterium which was isolated from a terrestrial hydrothermal spring. M. humiferrea is able to use humic acid or 10-anthraquinone-2,6-disulfonate as an electron-shuttling compound for growth and Fe(III) reduction. The genome has a size of 2.629 Mb and contains 2,668 predicted protein-coding genes.

Stereoisomer‐specific reprogramming of a bacterial flagellin sialyltransferase

“…Upon inspection of the predicted Leg biosynthesis clusters in the genomes of the Gram‐negative bacteria A. baumannii LAC‐4 (GCA_000786735.1; Ou et al , 2015) and Pseudomonas sp . Irchel 3E13 (GCA_900187455.1), as well as that of the Gram‐positive bacteria Geobacillus kaustophilus HTA426 (Takami et al , 2004; Khairnar et al , 2020) and Moorella humiferrea DSM 23265 (Poehlein et al , 2018), we noted that PtmE orthologs are also encoded in these Leg biosynthesis clusters.…”

“…Upon inspection of the predicted Leg biosynthesis clusters in the genomes of the Gramnegative bacteria A. baumannii LAC-4 (GCA_000786735.1; Ou et al, 2015) and Pseudomonas sp. Irchel 3E13 (GCA_900187455.1), as well as that of the Gram-positive bacteria Geobacillus kaustophilus HTA426 (Takami et al, 2004;Khairnar et al, 2020) and Moorella humiferrea DSM 23265 (Poehlein et al, 2018), we noted that PtmE orthologs are also encoded in these Leg biosynthesis clusters. If Bresu_3267 (henceforth LegX Bs /PtmE Bs ) indeed acts in Leg biosynthesis, then it might be possible to restore motility to DlegX cells by heterologous expression of LegX/PtmE orthologs (Fig 6A and B), akin to the heterologous complementation of DlegI cells.…”

Genetic glyco-profiling and rewiring of insulated flagellin glycosylation pathways

“…Upon inspection of the predicted Leg biosynthesis clusters in the genomes of the Gram-negative bacteria A. baumannii LAC-4 (GCA_000786735.1)[38] and P. sp . Irchel 3E13 (GCA_900187455.1), as well as that of the Gram-positive bacteria Geobacillus kaustophilus HTA426 [26, 39] and Moorella humiferrea DSM 23265 [40], we noted the presence of one gene encoding an ortholog of PtmE, an enzyme that was used in the enzymatic reconstitution of Leg biosynthesis in vitro using enzymes encoded in C. jejuni 11168[22]. In these experiments PtmE, a putative guanylyltransferase of GlcN-1-P (α-D-glucosamine-1-phosphate), promoted the production of GDP-GlcNAc in vitro (Figure 5A).…”