Draft Genome Sequence of the Animal and Human Pathogen
            <i>Malassezia pachydermatis</i>
            Strain CBS 1879

Triana, Sergio; González, Ainhoa; Ohm, Robin A.; Wösten, Han A. B.; Cock, Hans de; Restrepo, Silvia; Celis, Adriana

doi:10.1128/genomea.01197-15

Cited by 30 publications

(30 citation statements)

References 19 publications

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“…Laccase-encoding genes in the genome assemblies of representative strains for the 14 sequenced Malassezia species were identified using as queries the Lac1 and Lac2 proteins of C. neoformans , and their start and the stop codons were predicted based on the respective annotated orthologs in M. sympodialis , M. globosa , and M. pachydermatis (12–14) (see Fig. S4 in the supplemental material).…”

Section: Resultsmentioning

confidence: 99%

“…Genomic studies of Malassezia have been conducted over the last decade. In 2007, the first genome sequence for the dandruff-associated species M. globosa was published (12), followed in 2013 by the genome of M. sympodialis (13) and that of M. pachydermatis in 2015 (14). Most recently, Wu and colleagues sequenced, assembled, and annotated genomes for all 14 known Malassezia species, including multiple strains of the most widely studied species ( M. furfur , M. globosa , M. sympodialis , and M. restricta ), for a total of 24 genomes sequenced (9).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus

Ianiri

Averette

Kingsbury

et al. 2016

mBio

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The genus Malassezia includes 14 species that are found on the skin of humans and animals and are associated with a number of diseases. Recent genome sequencing projects have defined the gene content of all 14 species; however, to date, genetic manipulation has not been possible for any species within this genus. Here, we develop and then optimize molecular tools for the transformation of Malassezia furfur and Malassezia sympodialis using Agrobacterium tumefaciens delivery of transfer DNA (T-DNA) molecules. These T-DNAs can insert randomly into the genome. In the case of M. furfur, targeted gene replacements were also achieved via homologous recombination, enabling deletion of the ADE2 gene for purine biosynthesis and of the LAC2 gene predicted to be involved in melanin biosynthesis. Hence, the introduction of exogenous DNA and direct gene manipulation are feasible in Malassezia species.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus

Ianiri

Averette

Kingsbury

et al. 2016

mBio

View full text Add to dashboard Cite

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“…In 2007 the first genome sequence of a Malassezia species ( M. globosa ) was published . A few years later, a similar analysis was performed for M. sympodialis and for M. pachydermatis . In 2015, a study reported the sequences, assemblage and annotations for the genomes of 14 Malassezia species, including multiple strains of the most relevant species in medical dermatology ( M. globosa , M. sympodialis , M. restricta and M. furfur ) …”

Section: The Genus Malassezia General Properties Phylogeny Genomicmentioning

confidence: 99%

Biology, diagnosis and treatment of Malassezia dermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology

Bond

Morris

Guillot

et al. 2020

Veterinary Dermatology

118

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Background -The genus Malassezia is comprised of a group of lipophilic yeasts that have evolved as skin commensals and opportunistic cutaneous pathogens of a variety of mammals and birds.Objectives -The objective of this document is to provide the veterinary community and other interested parties with current information on the ecology, pathophysiology, diagnosis, treatment and prevention of skin diseases associated with Malassezia yeasts in dogs and cats.Methods and material -The authors served as a Guideline Panel (GP) and reviewed the literature available prior to October 2018. The GP prepared a detailed literature review and made recommendations on selected topics. The World Association of Veterinary Dermatology (WAVD) Clinical Consensus Guideline committee provided guidance and oversight for this process. The document was presented at two international meetings of veterinary dermatology societies and one international mycology workshop; it was made available for comment on the WAVD website for a period of six months. Comments were shared with the GP electronically and responses incorporated into the final document.Conclusions and clinical importance -There has been a remarkable expansion of knowledge on Malassezia yeasts and their role in animal disease, particularly since the early 1990's. Malassezia dermatitis in dogs and cats has evolved from a disease of obscurity and controversy on its existence, to now being a routine diagnosis in general veterinary practice. Clinical signs are well recognised and diagnostic approaches are well developed. A range of topical and systemic therapies is known to be effective, especially when predisposing factors are identified and corrected.

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“…Among them, M. pachydermatis is the only member that does not require lipid supplementation for growth on Sabouraud glucose agar [1]. Recently, it has been shown that the gene encoding the fatty acid synthase is missing in M. pachydermatis [2] and fatty acid requirement for Electronic supplementary material The online version of this article (doi:10.1007/s11046-016-0026-3) contains supplementary material, which is available to authorized users. growth in a defined medium has been reported for this species [3].…”

Section: Introductionmentioning

confidence: 99%

Cryptic Diversity of Malassezia pachydermatis from Healthy and Diseased Domestic Animals

2016

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Malassezia pachydermatis is part of the normal cutaneous microbiota of wild and domestic carnivores. However, under certain conditions this yeast can overproliferate and cause several diseases in its host, mainly otitis and dermatitis in dogs. The aim of this study was to conduct a molecular characterization of M. pachydermatis isolates from healthy and diseased domestic animals, in order to assess the molecular diversity and phylogenetic relationship within this species. The large subunit (LSU) and the internal transcribed spacer (ITS) of ribosomal RNA, chitin synthase 2 (CHS2) and β-tubulin genes from sixteen strains isolated from dogs, cats, a goat, a pig and a horse were sequenced. A different number of types of sequences were identified for each target gene, including some types described for the first time. Five sequence types were characterized for the LSU, eleven for the ITS region, nine for CHS2 and eight for β-tubulin. A multilocus analysis was performed including the four genes, and the resulting phylogenetic tree revealed fifteen genotypes. Genotypes were distributed in two well-supported clades. One clade comprised strains isolated from different domestic animals and a strongly supported cluster constituted by strains isolated from cats. The second clade included strains isolated mainly from dogs and an outlier strain isolated from a horse. No apparent association could be observed between the health status of the animal hosts and concrete strains. The multilocus phylogenetic analysis is a useful tool to assess the intraspecific variation within this species and could help understand the ecology, epidemiology and speciation process of M. pachydermatis.

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Draft Genome Sequence of the Animal and Human Pathogen Malassezia pachydermatis Strain CBS 1879

Cited by 30 publications

References 19 publications

Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus

Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus

Biology, diagnosis and treatment of Malassezia dermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology

Cryptic Diversity of Malassezia pachydermatis from Healthy and Diseased Domestic Animals

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