Draft genome sequence of the producer strain of aureocin 4181, an antimicrobial peptide with antagonistic activity against multidrug-resistant staphylococci

Marques-Bastos, Selda Loase Salustiano; Coelho, Marcus Lívio Varella; Santos, Ilana Nascimento de Sousa; Farias, Felipe Miceli de; Francisco, Márcia Silva; Albano, Rodolpho Mattos; Alviano, Celuta Sales; Bastos, Maria do Carmo de Freire

doi:10.1016/j.jgar.2020.10.015

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…High-quality reads were assembled using SPAdes v3.13.0 [ 53 ]. Potential protein-coding genes were predicted using RAST, and the protein functions were annotated using the protein basic local alignment search tool (BLASTp) against the non-redundant protein sequences (nr) database [ 54 ]. Genome functional maps were produced by a lab-built script and modified using Inkscape 0.92.3.0.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Genomics Analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Han

Zhang

et al. 2022

IJMS

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The spread of multidrug-resistant Klebsiella pneumoniae (MDR-KP) has become an emerging threat as a result of the overuse of antibiotics. Bacteriophage (phage) therapy is considered to be a promising alternative treatment for MDR-KP infection compared with antibiotic therapy. In this research, a lytic phage BUCT610 was isolated from hospital sewage. The assembled genome of BUCT610 was 46,774 bp in length, with a GC content of 48%. A total of 83 open reading frames (ORFs) and no virulence or antimicrobial resistance genes were annotated in the BUCT610 genome. Comparative genomics and phylogenetic analyses showed that BUCT610 was most closely linked with the Vibrio phage pYD38-A and shared 69% homology. In addition, bacteriophage BUCT610 exhibited excellent thermal stability (4–75 °C) and broad pH tolerance (pH 3–12) in the stability test. In vivo investigation results showed that BUCT610 significantly increased the survival rate of Klebsiella pneumonia-infected Galleria mellonella larvae from 13.33% to 83.33% within 72 h. In conclusion, these findings indicate that phage BUCT610 holds great promise as an alternative agent with excellent stability for the treatment of MDR-KP infection.

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Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Genomics Analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Han

Zhang

et al. 2022

IJMS

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“…In Staphylococcus aureus 4181, a strain involved in bovine mastitis, a bacteriocin-denominated aureocin 4181, is involved in resistance against multidrug-resistant (MDR) staphylococci. Interestingly, both BAGEL4 and antiSMASH v.5.0 were implemented in the identification of the gene cluster coding for aureocin 4181 45 , 46 . The findings were confirmed in the wet lab.…”

Section: Discovery Of Bioactive Bacterial Products Driven By Genome M...mentioning

confidence: 99%

Biological Dark Matter Exploration using Data Mining for the Discovery of Antimicrobial Natural Products

2022

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The discovery of novel antimicrobials has significantly slowed down over the last three decades. At the same time, humans rely increasingly on antimicrobials because of the progressive antimicrobial resistance in medical practices, human communities, and the environment. Data mining is currently considered a promising option in the discovery of new antibiotics. Some of the advantages of data mining are the ability to predict chemical structures from sequence data, anticipation of the presence of novel metabolites, the understanding of gene evolution, and the corroboration of data from multiple omics technologies. This review analyzes the state-of-the-art for data mining in the fields of bacteria, fungi, and plant genomic data, as well as metabologenomics. It also summarizes some of the most recent research accomplishments in the field, all pinpointing to innovation through uncovering and implementing the next generation of antimicrobials.

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Fighting antibiotic resistance in the local management of bovine mastitis

Touza-Otero,

Landin,

Diaz-Rodriguez

2024

Biomedicine & Pharmacotherapy

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Draft genome sequence of the producer strain of aureocin 4181, an antimicrobial peptide with antagonistic activity against multidrug-resistant staphylococci

Cited by 5 publications

References 10 publications

Characterization and Comparative Genomics Analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Characterization and Comparative Genomics Analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Biological Dark Matter Exploration using Data Mining for the Discovery of Antimicrobial Natural Products

Fighting antibiotic resistance in the local management of bovine mastitis

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