Draft Genome Sequence of the Strain Francisella tularensis subsp.
 mediasiatica
 240, Isolated in Kazakhstan

Куйбагаров, Марат; Kairzhanova, A.D.; Vergnaud, Gilles; Amirgazin, Asylulan; Lukhnova, Larissa; Izbanova, Uinkul; Ramankulov, Yerlan; Shevtsov, Alexandr

doi:10.1128/mra.00766-20

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…WGS data from three strains were previously published [2,5]. Strains of Central Asian (M.I) and Altaic (M.II) origin form two clusters separated by 116 SNPs (Figure 3) in agreement with previous analyses [2,11]. The M.II group in turn is split in two.…”

Section: Whole-genome Sequencing (Wgs) and Phylogenetic Analysissupporting

confidence: 88%

The First Finding of Francisella Tularensis Subsp. Mediasiatica in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Timofeev¹,

Бахтеева²,

Мокриевич³

et al. 2022

Preprint

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Tularemia is a severe infectious disease caused by the Gram-negative bacteria Francisella tularensis. F. tularensis is currently divided into three subspecies, holarctica, tularensis and mediasiatica which differ in their virulence and geographic distribution. Subspecies mediasiatica is the least studied because of its very low documented virulence for humans and limited geographic distribution. It was discovered in sparsely populated regions of Central Asia. Since 2011, a new subsp. mediasiatica lineage was identified in Altai (Russia). In 2021, we isolated one subsp. mediasiatica strain in Krasnoyarsk Territory. In spite of its geographic origin, 500 km east from Altai, this strain belongs to the Altai lineage and contributes surprisingly little genetic diversity to previous knowledge. This improved knowledge of the phylogeography of subsp. mediasiatica led us to propose a scenario in which the two zoonotic lineages, holarctica and tularensis, independently emerged from the mediasiatica lineage in Siberia or Central Asia, and make predictions which will allow to challenge this hypothesis

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Section: Whole-genome Sequencing (Wgs) and Phylogenetic Analysissupporting

confidence: 88%

The First Finding of Francisella Tularensis Subsp. Mediasiatica in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Timofeev¹,

Бахтеева²,

Мокриевич³

et al. 2022

Preprint

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“…F. tularensis subsp. mediasiatica is restricted to central Asia and Russia, and has never been associated with human infections [ 4 ]. F. tularensis subsp.…”

Section: Introductionmentioning

confidence: 99%

Presence of Francisella tularensis subsp. holarctica DNA in the Aquatic Environment in France

et al. 2021

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In 2018, the incidence of tularemia increased twofold in the west of France, with many pneumonic forms, suggesting environmental sources of infection. We investigated the presence of Francisella tularensis subsp. holarctica and other Francisella species DNA in the natural aquatic environment of this geographic area. Two sampling campaigns, in July 2019 and January 2020, allowed the collection of 87 water samples. Using a combination of real-time PCR assays, we tested the presence of either Francisella sp., F. tularensis/F. novicida, and F. tularensis subsp. holarctica, the latter being the only tularemia agent in Europe. Among 57 water samples of the first campaign, 15 (26.3%) were positive for Francisella sp., nine (15.8%) for F. tularensis and/or F. novicida, and four (7.0%) for F. tularensis subsp. holarctica. Ratios were 25/30 (83.3%), 24/30 (80.0%), and 4/30 (13.3%) for the second campaign. Among the thirty sites sampled during the two campaigns, nine were positive both times for Francisella sp., seven for F. tularensis and/or F. novicida, and one for F. tularensis subsp. holarctica. Altogether, our study reveals a high prevalence of Francisella sp. DNA (including the tularemia agent) in the studied aquatic environment. This aquatic environment could therefore participate in the endemicity of tularemia in the west of France.

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Genetic Homogeneity of Francisella tularensis subsp. mediasiatica Strains in Kazakhstan

Shevtsov,

Izbanova,

Amirgazin

et al. 2024

Pathogens

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Tularemia is an acute febrile disease caused by the Gram-negative bacillus Francisella tularensis. Based on genetic and phenotypic characteristics, three subspecies are distinguished: tularensis, holarctica, and mediasiatica. F. tularensis subsp. mediasiatica remains the least studied subspecies. Over the past decade, new foci of distribution of F. tularensis subsp. mediasiatica have been discovered in Russia (Siberia), expanding the possible distribution area by thousands of kilometers. This article provides whole genome single nucleotide polymorphism (wgSNP) and polymorphic tandem repeats (MLVA) analyses of 28 mediasiatica strains isolated between 1965 and 2004 in Kazakhstan. Despite high genetic homogeneity, MLVA with eleven loci (MLVA11) demonstrates a high discriminatory ability (diversity index, 0.9497). The topological structure of the trees based on wgSNP and MLVA is not comparable; however, clustering remains congruent for most outbreaks, with the exception of two strains from one outbreak that are identical in terms of wgSNP but differ at three tandem repeat loci. Based on wgSNP, the strains are assigned to one of the three currently known mediasiatica sublineages, lineage M.I, together with other historical strains maintained in collections in Russia and Sweden. wgSNP shows limited previously unknown genetic diversity, with the M.I lineage size being only 118 SNPs. The wgSNP genotype is not strongly correlated with year and place of isolation.

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Draft Genome Sequence of the Strain Francisella tularensis subsp. mediasiatica 240, Isolated in Kazakhstan

Abstract: Francisella tularensis subsp. mediasiatica is the least studied among the four F. tularensis subspecies. We present here the genome data of F. tularensis subsp. mediasiatica 240, isolated in the southern region of Kazakhstan.

Cited by 3 publications

References 10 publications

The First Finding of <em>Francisella Tularensis</em> Subsp. <em>Mediasiatica</em> in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

The First Finding of <em>Francisella Tularensis</em> Subsp. <em>Mediasiatica</em> in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Presence of Francisella tularensis subsp. holarctica DNA in the Aquatic Environment in France

Genetic Homogeneity of Francisella tularensis subsp. mediasiatica Strains in Kazakhstan

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