Draft Genome Sequence of the Live Vaccine Strain Brucella abortus 82

Shevtsov, Alexandr; Tarlykov, Pavel; Zholdybayeva, Elena; Shevtsova, E. F.; Momynkulov, Dauren; Sytnik, Igor; Karibaev, Talgat; Chsherbakov, Andrey; Momynaliev, K. Т.

doi:10.1128/genomea.01101-13

Cited by 7 publications

(5 citation statements)

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“…Since the mid-70s, B . abortus strain 82 has been used to vaccinate all groups of cattle along with state-wide test and slaughter program, which led to a gradual decrease of incidence in herds [39, 40], reaching its minimum (1.4%) in 1989. Hypothetically, continuation of this combined strategy would eradicate brucellosis from cattle.…”

Section: Resultsmentioning

confidence: 99%

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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Brucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan. We analyzed official data on the incidence of animal brucellosis in Kazakhstan. The records span more than 70 years of anti-brucellosis campaigns, and contain a brief description of the applied control strategies, their effectiveness, and their impact on the incidence in humans. The MLVA-16 method was used to type 94 strains of B. abortus and serial passages of B. abortus 82, a strain used in vaccines. MLVA-8 and MLVA-11 analyses clustered strains into a total of four and seven genotypes, respectively; it is the first time that four of these genotypes have been described. MLVA-16 analysis divided strains into 28 distinct genotypes having genetic similarity coefficient that varies from 60 to100% and a Hunter & Gaston diversity index of 0.871. MST analysis reconstruction revealed clustering into "Kazakhstani-Chinese (Central Asian)", "European" and "American" lines. Detection of multiple genotypes in a single outbreak confirms that poorly controlled trade of livestock plays a crucial role in the spread of infection. Notably, the MLVA-16 profile of the B. abortus 82 strain was unique and did not change during 33 serial passages. MLVA genotyping may thus be useful for epidemiological monitoring of brucellosis, and for tracking the source(s) of infection. We suggest that countrywide application of MLVA genotyping would improve the control of brucellosis in Kazakhstan.

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Section: Resultsmentioning

confidence: 99%

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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“…In the recent past, there has been an increase in alarming rate of seroprevalence in strains affecting both human and cattle, with the latter proving to be disastrous for agricultural growth (Moreno 2014). Although complete genomes were obtained for many Brucella species, however not all these data are adequate to understand the bacterial pathogenesis leading to genomewide association studies (Shevtsov et al 2013;Shome et al 2013). Besides, our understanding of genetic variation and epidemiology advanced with next generation sequencing (NGS) of Brucella spp., and provided a challenge to understand the sequences of these genomes better.…”

Section: Introductionmentioning

confidence: 99%

On Brucella pathogenesis: looking for the unified challenge in systems and synthetic biology

Chiliveru¹,

Appari

Suravajhala³

2014

Syst Synth Biol

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Brucellosis is a zoonotic infection transmitted to humans from infected animals and is one of the widely spread zoonoses. Recently, six species were recognized within the genus Brucella wherein B. melitensis, B. suis and B. abortus are considered virulent for humans. While these species differ phenotypically by their pattern of metabolic activities, there has been an imperative need to understand pathogenesis of Brucella species. It has been foreseen that creating a human vaccine for Brucellosis would entail decreased dose of antibiotics. However the emerging role of Brucella pathogenesis still centers on isolation of the organism and various diagnostic tests thereby leading to varying strategies of treatment cycle. In view of disease heterogeneity, we focus systems and synthetic biology challenges that might improve our understanding the Brucella pathogenesis.

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“…Scraping and walking jar therapy has been proved to exhibit a significant effect on suffocation‐like pain in the back complicated by brucellosis, with the advantages of simple operation and nontoxic side effects and is thus worthy of being promoted in the clinic. Using fire acupuncture to treat joint effusion complicated by brucellosis can reach an 87.5% cure rate [48]. Patients with bone and joint pain complicated by brucellosis can be treated by identifying and selecting points and methods [49].…”

Section: Current Treatment Options For Brucellosismentioning

confidence: 99%

Research progress in the therapy of brucellosis

Wang

Peng

2023

Animal Research and One Health

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Brucellosis is a zoonotic bacterial disease posing serious threats to the health of humans and animals. Currently, effective therapeutics available for brucellosis are in urgent demand. Brucellosis is transmitted to humans through diet or contact with infected animals. Several live‐attenuated vaccine strains are commercially available for animals, such as Brucella melitensis Rev.1, Brucella abortus S19, and RB51. However, no secure and effective vaccine for human beings has been developed yet against brucellosis, for which antibiotic therapy is the most frequently used and valid. Nevertheless, excessive antibiotics could prohibit the sensitivity of Brucella and develop resistance in humans. In addition, other clinical treatments, such as surgical treatment, have little effect on brucellosis. Therefore, novel treatment protocols should be explored. Based on numerous experimental studies, it is speculated that brucellosis can be fundamentally treated by inhibiting the activation of the type IV secretion system and changing the acidic environment of eBCV. In addition, previous literature showed that traditional Chinese medicine therapy also had certain efficacy, providing a new perspective angle for the exploration of treatment options.

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Draft Genome Sequence of the Live Vaccine Strain Brucella abortus 82

Cited by 7 publications

References 4 publications

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

On Brucella pathogenesis: looking for the unified challenge in systems and synthetic biology

Research progress in the therapy of brucellosis

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