Draft Genome Sequence of the Mannitol-Producing Strain Lactobacillus mucosae CRL573

Bleckwedel, Juliana; Terán, Lucrecia C.; Bonacina, Julieta; Saavedra, Lucila; Mozzi, Fernanda; Raya, Raúl R.

doi:10.1128/genomea.01292-14

Cited by 9 publications

(12 citation statements)

References 10 publications

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“…The average genomic size of 93 L. mucosae was 2.11 Mb with a 48.07% average of the GC content, which was consistent with previous reports [12,13]. Unprecedented, the current work on the genome-wide 101 L. mucosae showed a trend of gradual closure of the genome [37].…”

Section: Discussionsupporting

confidence: 88%

“…Previously, little research had focused on CRISPR in L. mucosae, and only two strains were analyzed. In L. mucosae LM1, two CRISPR-Cas loci (type I and type II) were found [6] while only CRISPR elements were detected in L. mucosae CRL573 [13]. Therefore, the current work specifically analyzed the CRISPR-Cas system in all those 93 strains of L. mucosae, of which 44% contained the complete CRISPR-Cas system.…”

Section: Discussionmentioning

confidence: 97%

“…L. mucosae AGR63 was reported to have the ability to produce only class III bacteriocin [58], and L. mucosae CRL573 was found to contain two potential enterostatin A operons (>10 kDa) [13]. Heretofore, little research has been done on the bacteriocin produced by L. mucosae.…”

Section: Discussionmentioning

confidence: 99%

“…A strain of L. mucosae isolated from goat milk was recently reported [11]. Although L. mucosae has been shown to have a number of benefits to human, only two human-derived strains have been reported yet, which were isolated from childhood feces [12,13]. This status quo may limit the excavation of other functions of L. mucosae, which requires more strains.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics Analysis of Lactobacillus mucosae from Different Niches

Jia

Yang

Ross

et al. 2020

Genes

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The potential probiotic benefits of Lactobacillus mucosae have received increasing attention. To investigate the genetic diversity of L. mucosae, comparative genomic analyses of 93 strains isolated from different niches (human and animal gut, human vagina, etc.) and eight strains of published genomes were conducted. The results showed that the core genome of L. mucosae mainly encoded translation and transcription, amino acid biosynthesis, sugar metabolism, and defense function while the pan-genomic curve tended to be close. The genetic diversity of L. mucosae mainly reflected in carbohydrate metabolism and immune/competitive-related factors, such as exopolysaccharide (EPS), enterolysin A, and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas. It was worth noting that this research firstly predicted the complete EPS operon shared among L. mucosae. Additionally, the type IIIA CRISPR-Cas system was discovered in L. mucosae for the first time. This work provided new ideas for the study of this species.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Genomics Analysis of Lactobacillus mucosae from Different Niches

Jia

Yang

Ross

et al. 2020

Genes

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“…While genomes sequenced using Roche 454 GS FLX continued to be assembled using Newbler assembler 62 , genomes sequenced using Ion Torrent systems were assembled using Newbler 57 , NGen (DNAStar) 53 61 , and Artemis being used for annotation and curation.…”

Section: Genomic Technologies In Probiotic Researchmentioning

confidence: 99%

Untitled

2018

IJPSR

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Probiotics are live microorganisms that confer many health benefits to the host when administered in adequate quantities. These health benefits have garnered much attention towards Probiotics and have given an impetus to their use as dietary supplements for the improvement of general health and as adjuvant therapies for certain diseases. The increased demand for probiotic products in the recent times has provided the thrust for probiotic research applied to several areas of human biology. The advances in genomic technologies have further facilitated the sequencing of the genomes of such probiotic bacteria and their genomic analyses to identify the genes that endow the beneficial effects they are known to exert. This work reviews the application of genomic strategies on probiotic bacteria, while providing the details about the probiotic strains whose genome sequences are available. It also consolidates the Genomic tools used for the sequencing, assembly and annotation of the probiotic genes and how it has helped in comparative genomic analyses.

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Functional Properties of Lactobacillus mucosae Strains Isolated from Brazilian Goat Milk

Moraes

Abreu

Egito

et al. 2016

Probiotics & Antimicro. Prot.

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The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties. Lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three Lb. mucosae strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests. The presence of genetic factors related to bile salt hydrolase production (bsh), intestinal adhesion properties (msa, map, mub, and ef-tu), virulence, and biogenic amine production were also verified. All strains exhibited the target map, mub, and ef-tu sequences; the msa gene was detected in CNPC006 and CNPC007 strains. Some of the searched sequences for virulence factors were detected, especially in the CNPC009 strain; all strains carried the hyl gene, related to the production of hyaluronidase. Lb. mucosae CNPC007 exhibited a high survival rate in simulated gastric and enteric conditions. Besides, all strains exhibited the bsh sequence, and CNPC006 and CNPC007 were able to deconjugate salts of glycodeoxycholic acid (GDC). Regarding technological properties for dairy product applications, a relatively higher milk acidification and clotting capacity, diacetyl production, and proteolytic activity were registered for CNPC007 in comparison to the other strains. Collectively, the results aim at Lb. mucosae CNPC007 as a promising probiotic candidate for application in dairy products, deserving further studies to confirm and explore its potential.

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Draft Genome Sequence of the Mannitol-Producing Strain Lactobacillus mucosae CRL573

Cited by 9 publications

References 10 publications

Comparative Genomics Analysis of Lactobacillus mucosae from Different Niches

Comparative Genomics Analysis of Lactobacillus mucosae from Different Niches

Untitled

Functional Properties of Lactobacillus mucosae Strains Isolated from Brazilian Goat Milk

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