Draft Genome Sequence of the Gammaproteobacterial Strain MOLA455, a Representative of a Ubiquitous Proteorhodopsin-Producing Group in the Ocean

Courties, Alicia; Riedel, Thomas; Jarek, Michael; Papadatou, Maria; Intertaglia, Laurent; Suzuki, Marcelino T.

doi:10.1128/genomea.01203-13

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…These genomes included data from P. temperata RCA23 and HTCC2255, belonging to two Roseobacter lineages, the RCA and NAC11-7 clusters. Other reference genomes used were the genomes of HTCC2207 und MOLA455, both members of the SAR92 clade (Stingl et al, 2007 ; Courties et al, 2014 ). Mapping the datasets on assembled metagenomic and metatranscriptomic data resulted in an overall alignment rate of more than 86.22% (see Table 5 ).…”

Section: Resultsmentioning

confidence: 99%

The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

Wemheuer

Hollensteiner

et al. 2015

Front. Microbiol.

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Phytoplankton blooms exhibit a severe impact on bacterioplankton communities as they change nutrient availabilities and other environmental factors. In the current study, the response of a bacterioplankton community to a Phaeocystis globosa spring bloom was investigated in the southern North Sea. For this purpose, water samples were taken inside and reference samples outside of an algal spring bloom. Structural changes of the bacterioplankton community were assessed by amplicon-based analysis of 16S rRNA genes and transcripts generated from environmental DNA and RNA, respectively. Several marine groups responded to bloom presence. The abundance of the Roseobacter RCA cluster and the SAR92 clade significantly increased in bloom presence in the total and active fraction of the bacterial community. Functional changes were investigated by direct sequencing of environmental DNA and mRNA. The corresponding datasets comprised more than 500 million sequences across all samples. Metatranscriptomic data sets were mapped on representative genomes of abundant marine groups present in the samples and on assembled metagenomic and metatranscriptomic datasets. Differences in gene expression profiles between non-bloom and bloom samples were recorded. The genome-wide gene expression level of Planktomarina temperata, an abundant member of the Roseobacter RCA cluster, was higher inside the bloom. Genes that were differently expressed included transposases, which showed increased expression levels inside the bloom. This might contribute to the adaptation of this organism toward environmental stresses through genome reorganization. In addition, several genes affiliated to the SAR92 clade were significantly upregulated inside the bloom including genes encoding for proteins involved in isoleucine and leucine incorporation. Obtained results provide novel insights into compositional and functional variations of marine bacterioplankton communities as response to a phytoplankton bloom.

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Section: Resultsmentioning

confidence: 99%

The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

Wemheuer

Hollensteiner

et al. 2015

Front. Microbiol.

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“…In addition, genome analyses revealed the potential of harvesting light for mixotrophic growth in several members of the OMG group, which made these isolates interesting study objects for further research (e.g., Stingl et al, 2007 ; Spring and Riedel, 2013 ). Genes involved in the complex pathway of aerobic anoxygenic photosynthesis were so far only found among members of the OM60/NOR5 clade (Spring et al, 2013 , 2014 ), whereas genes for the synthesis of proteorhodopsin, a light-driven proton pump, seems to be more widely spread and were identified in the genomes of representatives of the SAR92 (Courties et al, 2014 ), BD1-7 (Oh et al, 2010a ), and OM60/NOR5 clades (Jang et al, 2011b ).…”

Section: Introductionmentioning

confidence: 99%

A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data

et al. 2015

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In recent years a large number of isolates were obtained from saline environments that are phylogenetically related to distinct clades of oligotrophic marine gammaproteobacteria, which were originally identified in seawater samples using cultivation independent methods and are characterized by high seasonal abundances in coastal environments. To date a sound taxonomic framework for the classification of these ecologically important isolates and related species in accordance with their evolutionary relationships is missing. In this study we demonstrate that a reliable allocation of members of the oligotrophic marine gammaproteobacteria (OMG) group and related species to higher taxonomic ranks is possible by phylogenetic analyses of whole proteomes but also of the RNA polymerase beta subunit, whereas phylogenetic reconstructions based on 16S rRNA genes alone resulted in unstable tree topologies with only insignificant bootstrap support. The identified clades could be correlated with distinct phenotypic traits illustrating an adaptation to common environmental factors in their evolutionary history. Genome wide gene-content analyses revealed the existence of two distinct ecological guilds within the analyzed lineage of marine gammaproteobacteria which can be distinguished by their trophic strategies. Based on our results a novel order within the class Gammaproteobacteria is proposed, which is designated Cellvibrionales ord. nov. and comprises the five novel families Cellvibrionaceae fam. nov., Halieaceae fam. nov., Microbulbiferaceae fam. nov., Porticoccaceae fam. nov., and Spongiibacteraceae fam. nov.

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“…Similarly, members of the Cellvibrionales order dominated the offshore area. These bacteria, generally affiliated to the Oligotrophic Marine Gammaproteobacteria (OMG) cluster (Cho and Giovannoni, 2004), are able to adapt to nutrient depletion and carbon limitation with the potential to harvest light for mixotrophic growth (Stingl et al, 2007;Spring and Riedel, 2013;Courties et al, 2014). Within our dataset, we also detected sequences related to the clades SAR92 and OM60 (NOR5).…”

Section: Discussionmentioning

confidence: 82%

Bacterioplankton Diversity and Distribution in Relation to Phytoplankton Community Structure in the Ross Sea surface waters

Cordone

d’Errico

et al. 2021

Preprint

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Primary productivity in the Ross Sea region is characterized by intense phytoplankton blooms whose temporal and spatial distribution are driven by changes in environmental conditions as well as interactions with the bacterioplankton community. Exchange of exudates, metabolism by-products and cofactors between the phytoplankton and the bacterioplankton communities drive a series of complex interactions affecting the micronutrient availability and co-limitation, as well as nutrient uptakes in Antarctic waters. Yet, the number of studies reporting the simultaneous diversity of the phytoplankton and bacterioplankton in Antarctic waters are limited. Here we report data on the bacterial diversity in relation to phytoplankton community in the surface waters of the Ross Sea during the austral summer 2017. Our results show partially overlapping bacterioplankton communities between the stations located in the Terra Nova Bay coastal waters and the Ross Sea open waters, suggesting that the two communities are subjected to different drivers. We show that the rate of diversity change between the two locations is influenced by both abiotic (salinity and the nitrogen to phosphorus ratio) and biotic (phytoplankton community structure) factors. Our data provides new insight into the coexistence of the bacterioplankton and phytoplankton in Antarctic waters.

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Draft Genome Sequence of the Gammaproteobacterial Strain MOLA455, a Representative of a Ubiquitous Proteorhodopsin-Producing Group in the Ocean

Abstract: Strain MOLA455 is a marine gammaproteobacterium isolated from the bay of Banyuls-sur-Mer, France. Here, we present its genome sequence and annotation. Genome analysis revealed the presence of genes associated with a possibly photoheterotrophic lifestyle that uses a proteorhodopsin protein.

Cited by 4 publications

References 11 publications

The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data

Bacterioplankton Diversity and Distribution in Relation to Phytoplankton Community Structure in the Ross Sea surface waters

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