Draft Genome Sequence of Serratia fonticola UTAD54, a Carbapenem-Resistant Strain Isolated from Drinking Water

Henriques, Isabel; Ramos, Rommel Thiago Jucá; Baraúna, Rafael Azevedo; Sá, Pablo Henrique de; Almeida, Diogo Marinho; Carneiro, Adriana Ribeiro; Barbosa, Silvanira; Pereira, Anabela; Alves, Artur; Saavedra, María José; Egas, Conceição; Silva, Artur M. S.; Correia, António

doi:10.1128/genomea.00970-13

Cited by 7 publications

(7 citation statements)

References 9 publications

(13 reference statements)

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“…36, 37 On the other hand, bla SFC and bla SFH , which were prevalent among the river Serratia isolates, have been described only once in environmental S. fonticola and not in clinical settings. 3, 38–40 Further studies are needed to elucidate the health risks posed by these CPE in surface waters.…”

Section: Resultsmentioning

confidence: 99%

Emergence of rare carbapenemases (FRI, GES-5, IMI, SFC, and SFH-1) in Enterobacterales isolated from surface waters in Japan

Gomi

Matsumura

Tanaka

et al. 2021

Preprint

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Objectives: Carbapenemase-producing Enterobacterales (CPE) pose serious threats to public health. Compared with clinical CPE, the genetic characteristics of environmental CPE are not well understood. This study aimed to characterize the genetic determinants of carbapenem resistance in CPE isolated from environmental waters in Japan. Methods: Eighty-five water samples were collected from rivers and a lake in Japan. CPE were identified using selective media, and genome sequencing was performed for the obtained isolates (n = 21). Results: Various rare/novel carbapenemases were identified: GES-5 in Raoultella planticola (n = 1), FRI-8 and FRI-11 in Enterobacter spp. (n = 8), IMI-22 and IMI-23 in Serratia ureilytica (n = 3), and SFC-1, SFC-2 and SFH-1 in Serratia fonticola (n = 9). Genomes of 11 isolates could be closed, allowing the elucidation of the genetic contexts of the carbapenemase genes. The blaGES-5 gene was located within a class 1 integron, In2071 (cassette array, blaGES-5-aacA3-aadA16), on a 33 kb IncP6 plasmid. The blaFRI-8 genes were carried on IncFII(Yp) plasmids ranging in size from 191 kb to 244 kb, and the blaFRI-11 genes were carried on 70 kb and 74 kb IncFII(pECLA)/IncR plasmids. The blaIMI-22 and blaIMI-23 genes were colocated on a 107 kb plasmid. The blaSFC and blaSFH-1 genes were found on putative genomic islands inserted at tRNA-Phe genes in chromosomes. Conclusions: This study revealed the presence of rare/novel carbapenemases among CPE in aquatic environments, suggesting that the environment may act as a potential reservoir of these minor carbapenemases.

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Section: Resultsmentioning

confidence: 99%

Emergence of rare carbapenemases (FRI, GES-5, IMI, SFC, and SFH-1) in Enterobacterales isolated from surface waters in Japan

Gomi

Matsumura

Tanaka

et al. 2021

Preprint

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“…Meanwhile, a large number of Serratia sp. were isolated from urinary tract clinical samples [7,8]. Manikandan and coworkers [9] reported that Serratia sp.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients

Hasson¹,

Al-Hamadani²,

Al-Azawi³

2018

Nano BioMed ENG

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Serratia fonticola and Pantoea sp. are gram negative bacteria belonging Enterobactericeae, which were considered opportunistic pathogens and resulted in a great number of cases of nosocomial infections with serious problems of multi-drug resistance, leading to increasing morbidity and mortality rate. Recently, they were recorded as biofilm producers. There were only a few studies about the capability of these bacteria of forming biofilm. So our aim was evaluate the occurrence of Serratia fonticola and Pantoea sp. biofilm former phenotypically and genetically with the determination of their abilities to multi-drug resistance. Serratia fonticola and Pantoea sp. isolated from urine catheterized patients who were hospitalized in Iraqi hospitals. They were then examined for detection of biofilm formation phenotypically by congo red and tissue culture plate methods and genetically by detecting SmaI and EsaI genes (quarm sensing genes) in Serratia fonticola and Pantoea sp. respectively by using polymerase chain reaction method and tested for antimicrobial susceptibility by disc diffusion and VITEK2 system according to Clinical and Laboratory Standards Institute (CLSI). Serratia fonticola at 3 and Pantoea sp. at 4 isolates revealed to possess the ability of forming biofilm which contained SmaI and EsaI genes with 100% resistance to most tested antibiotics except imipenem and azithromycin. SmaI and EsaI genes are present in Serratia fonticola and Pantoea sp. respectively, and are responsible for biofilm formation and considered as indicator; biofilm formation is a strong cause of multidrug resistance in bacteria.

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“…Studies reporting ESBL- and carbapenemase-producing bacteria in drinking water in high-income countries are extremely rare. CTX-M-producing E. coli was discovered in drinking water in France in a single water sample 10 , and carbapenemase-producing Serratia fonticola has previously been reported in drinking water from Portugal 32 , as have non-fermenting intrinsic carbapenemase-producers 13 . To our knowledge, this is the first extensive study of drinking water from multiple regions in a high income country that has revealed the geographically widespread distribution of ESBL- and carbapenemase-producing isolates of serious clinical concern.…”

Section: Discussionmentioning

confidence: 99%

Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in U.S. drinking water

Tanner

VanDerslice

Goel

et al. 2019

Sci Rep

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Community-associated acquisition of extended-spectrum beta-lactamase- (ESBL) and carbapenemase-producing Enterobacteriaceae has significantly increased in recent years, necessitating greater inquiry into potential exposure routes, including food and water sources. In high-income countries, drinking water is often neglected as a possible source of community exposure to antibiotic-resistant organisms. We screened coliform-positive tap water samples (n = 483) from public and private water systems in six states of the United States for bla CTX-M , bla SHV , bla TEM , bla KPC , bla NDM , and bla OXA-48 -type genes by multiplex PCR. Positive samples were subcultured to isolate organisms harboring ESBL or carbapenemase genes. Thirty-one samples (6.4%) were positive for bla CTX-M , ESBL-type bla SHV or bla TEM , or bla OXA-48 -type carbapenemase genes, including at least one positive sample from each state. ESBL and bla OXA-48 -type Enterobacteriaceae isolates included E. coli, Kluyvera , Providencia , Klebsiella , and Citrobacter species. The bla OXA-48 -type genes were also found in non-fermenting Gram-negative species, including Shewanella, Pseudomonas and Acinetobacter . Multiple isolates were phenotypically non-susceptible to third-generation cephalosporin or carbapenem antibiotics. These findings suggest that tap water in high income countries could serve as an important source of community exposure to ESBL and carbapenemase genes, and that these genes may be disseminated by non- Enterobacteriaceae that are not detected as part of standard microbiological water quality testing.

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Draft Genome Sequence of Serratia fonticola UTAD54, a Carbapenem-Resistant Strain Isolated from Drinking Water

Cited by 7 publications

References 9 publications

Emergence of rare carbapenemases (FRI, GES-5, IMI, SFC, and SFH-1) in Enterobacterales isolated from surface waters in Japan

Emergence of rare carbapenemases (FRI, GES-5, IMI, SFC, and SFH-1) in Enterobacterales isolated from surface waters in Japan

Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients

Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in U.S. drinking water

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