Draft Genome Sequence of Rhodococcus rhodochrous Strain ATCC 21198

Shields-Menard, Sara A.; Brown, Steven D.; Klingeman, Dawn M.; Indest, Karl J.; Hancock, Dawn E.; Wewalwela, Jayani J.; French, W. Todd; Donaldson, Janet R.

doi:10.1128/genomea.00054-14

Cited by 11 publications

(5 citation statements)

References 10 publications

(10 reference statements)

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“…Annotation of the draft genome sequence of KG-21 also revealed several genes that may help produce large amounts of lipids in the form of triacylglycerols (TAGs), due to the high activity of enzymes, like wax ester synthase/diacylglycerol acyltransferase, acetyl-coenzyme A (CoA) carboxylase, acyl carrier proteins, (1-acylglycerol-phosphate) acyltransferase, glycerol kinase, and glycerol-3-phosphate dehydrogenase, as reported earlier for R. rhodochrous strain ATCC 21198 ( 9 ). In addition, it also has genes for cytochrome P450 monooxygenase and several peroxidases, oxygenases, and lipases.…”

Section: Genome Announcementsupporting

confidence: 59%

Draft Genome Sequence of Rhodococcus rhodochrous Strain KG-21, a Soil Isolate from Oil Fields of Krishna-Godavari Basin, India

Dawar

Aggarwal

2015

Genome Announc

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Section: Genome Announcementsupporting

confidence: 59%

Draft Genome Sequence of Rhodococcus rhodochrous Strain KG-21, a Soil Isolate from Oil Fields of Krishna-Godavari Basin, India

Dawar

Aggarwal

2015

Genome Announc

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“…When grown in liquid cultures, the growth rate on guaiacol was 0.2 h −1 , which is slightly higher than the growth of the same strain on vanillin (0.127 h −1 ) and 4-HBA (0.126 h −1 ) [ 17 ]. Some species of Rhodococcus have previously been reported to be able to grow on guaiacol [ 78 – 80 ] or possess genes related to guaiacol breakdown [ 81 ]. The sequence of the enzyme (Cytochrome P450, UniProtKB: W4A0P3) responsible for the conversion of guaiacol to catechol from R. rhodochrous (ATCC 21198) [ 81 ] was blasted against the genome of the R. opacus (taxid: 37919) and an identity of 81% was found, which strongly indicates the presence of a similar protein.…”

Section: Discussionmentioning

confidence: 99%

“…Some species of Rhodococcus have previously been reported to be able to grow on guaiacol [ 78 – 80 ] or possess genes related to guaiacol breakdown [ 81 ]. The sequence of the enzyme (Cytochrome P450, UniProtKB: W4A0P3) responsible for the conversion of guaiacol to catechol from R. rhodochrous (ATCC 21198) [ 81 ] was blasted against the genome of the R. opacus (taxid: 37919) and an identity of 81% was found, which strongly indicates the presence of a similar protein. When the same enzyme was blasted against the genome of KT2440, no similar protein was found and with P. fluorescens (taxid: 294) only a protein with low identity (28%) was found.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Bacterial conversion of depolymerized Kraft lignin

Ravi

Abdelaziz

Nöbel

et al. 2018

Biotechnol Biofuels

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BackgroundLignin is a potential feedstock for microbial conversion into various chemicals. However, the degradation rate of native or technical lignin is low, and depolymerization is needed to obtain reasonable conversion rates. In the current study, base-catalyzed depolymerization—using NaOH (5 wt%)—of softwood Kraft lignin was conducted in a continuous-flow reactor system at temperatures in the range 190–240 °C and residence times of 1 or 2 min. The ability of growth of nine bacterial strains belonging to the genera Pseudomonas and Rhodococcus was tested using the alkaline-treated lignin as a sole carbon source.ResultsPseudomonas fluorescens and Rhodococcus opacus showed the best growth of the tested species on plates with lignin. Further evaluation of P. fluorescens and R. opacus was made in liquid cultivations with depolymerized lignin (DL) at a concentration of 1 g/L. Size exclusion chromatography (SEC) showed that R. opacus consumed most of the available lower molecular weight compounds (approximately 0.1–0.4 kDa) in the DL, but the weight distribution of larger fractions was almost unaffected. Importantly, the consumed compounds included guaiacol—one of the main monomers in the DL. SEC analysis of P. fluorescens culture broth, in contrast, did not show a large conversion of low molecular weight compounds, and guaiacol remained unconsumed. However, a significant shift in molecular weight distribution towards lower average weights was seen.ConclusionsRhodococcus opacus and P. fluorescens were identified as two potential microbial candidates for the conversion/consumption of base-catalyzed depolymerized lignin, acting on low and high molecular weight lignin fragments, respectively. These findings will be of relevance for designing bioconversion of softwood Kraft lignin.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1240-7) contains supplementary material, which is available to authorized users.

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“…It is also possible that PrMO is not the only propane‐oxidizing enzyme utilized by the biostimulated microorganisms to oxidize propane. Several recent reports have indicated that propane‐oxidizing bacteria often possess and express genes for more than one hydrocarbon‐oxidizing enzyme during growth on this substrate (Crombie and Murrell 2014; Shields‐Menard et al ; Cappelletti et al ; He et al ; Bennett et al ).…”

Section: Introductionmentioning

confidence: 99%

Concurrent Treatment of 1,4‐Dioxane and Chlorinated Aliphatics in a Groundwater Recirculation System Via Aerobic Cometabolism

Chu¹,

Bennett²,

Dolan³

et al. 2018

Groundwater Monitoring Rem

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This research demonstrates that groundwater contaminated by a relatively dilute but persistent concentration of 1,4‐dioxane (1,4‐D), approximately 60 μg/L, and chlorinated aliphatic co‐contaminants (1.4 to 10 μg/L) can be efficiently and reliably treated by in situ aerobic cometabolic biodegradation (ACB). A field trial lasting 265 days was conducted at Operable Unit D at the former McClellan Air Force Base and involved establishing an in situ ACB reactor through amending recirculated groundwater with propane and oxygen. The stimulated indigenous microbial population was able to consistently degrade 1,4‐D to below 3 μg/L while the co‐contaminants trichloroethene (TCE) and 1,2‐dichloroethane (1,2‐DCA) were decreased to below 1 μg/L and 0.18 μg/L, respectively. A stable treatment efficiency of more than 95% removal for 1,4‐D and 1,2‐DCA and of more than 90% removal for TCE was achieved. High treatment efficiencies for 1,4‐D and all co‐contaminants were sustained even without propane and oxygen addition for a 2‐week period.

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Draft Genome Sequence of Rhodococcus rhodochrous Strain ATCC 21198

Abstract: Rhodococcus rhodochrous is a Gram-positive red-pigmented bacterium commonly found in the soil. The draft genome sequence for R. rhodochrous strain ATCC 21198 is presented here to provide genetic data for a better understanding of its lipid-accumulating capabilities.

Cited by 11 publications

References 10 publications

Draft Genome Sequence of Rhodococcus rhodochrous Strain KG-21, a Soil Isolate from Oil Fields of Krishna-Godavari Basin, India

Draft Genome Sequence of Rhodococcus rhodochrous Strain KG-21, a Soil Isolate from Oil Fields of Krishna-Godavari Basin, India

RETRACTED ARTICLE: Bacterial conversion of depolymerized Kraft lignin

Concurrent Treatment of 1,4‐Dioxane and Chlorinated Aliphatics in a Groundwater Recirculation System Via Aerobic Cometabolism

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