Draft genome sequence of Rhodococcus erythropolis B7g, a biosurfactant producing actinobacterium

Retamal-Morales, Gerardo; Heine, Thomas; Tischler, Judith S.; Erler, Beate; Gröning, Janosch A. D.; Kaschabek, Stefan R.; Schlömann, Michael; Levicán, Gloria; Tischler, Dirk

doi:10.1016/j.jbiotec.2018.06.001

Cited by 22 publications

(12 citation statements)

References 24 publications

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“…The trehalose-6-phosphate synthase, OtsA, was reported to be the key enzyme involved in the synthesis of the sugar residue of the trehalose lipids biosynthesis (Tischler et al 2013 ). The presence of an alternative trehalose biosynthesis pathway mediated by maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ) enzymes was predicted in the biosurfactant producer R. erythropolis B7g (Retamal-Morales et al 2018a ). This pathway for trehalose biosynthesis uses oligo/polymalto-dextrins/glycogen as substrate (Tropis et al 2005 ).…”

Section: Biosurfactantsmentioning

confidence: 99%

Biotechnology of Rhodococcus for the production of valuable compounds

Cappelletti

Presentato

Piacenza

et al. 2020

Appl Microbiol Biotechnol

106

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Bacteria belonging to Rhodococcus genus represent ideal candidates for microbial biotechnology applications because of their metabolic versatility, ability to degrade a wide range of organic compounds, and resistance to various stress conditions, such as metal toxicity, desiccation, and high concentration of organic solvents. Rhodococcus spp. strains have also peculiar biosynthetic activities that contribute to their strong persistence in harsh and contaminated environments and provide them a competitive advantage over other microorganisms. This review is focused on the metabolic features of Rhodococcus genus and their potential use in biotechnology strategies for the production of compounds with environmental, industrial, and medical relevance such as biosurfactants, bioflocculants, carotenoids, triacylglycerols, polyhydroxyalkanoate, siderophores, antimicrobials, and metal-based nanostructures. These biosynthetic capacities can also be exploited to obtain high value-added products from low-cost substrates (industrial wastes and contaminants), offering the possibility to efficiently recover valuable resources and providing possible waste disposal solutions. Rhodococcus spp. strains have also recently been pointed out as a source of novel bioactive molecules highlighting the need to extend the knowledge on biosynthetic capacities of members of this genus and their potential utilization in the framework of bioeconomy. Key points • Rhodococcus possesses promising biosynthetic and bioconversion capacities. • Rhodococcus bioconversion capacities can provide waste disposal solutions. • Rhodococcus bioproducts have environmental, industrial, and medical relevance.

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Section: Biosurfactantsmentioning

confidence: 99%

Biotechnology of Rhodococcus for the production of valuable compounds

Cappelletti

Presentato

Piacenza

et al. 2020

Appl Microbiol Biotechnol

106

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“…The DNA library preparation, genome sequencing, assembly, annotation and analysis were done as described previously [16]. Therewith a genome coverage of 247x for strain H3 and 249x for strain RGB was obtained, respectively.…”

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confidence: 99%

Draft genomes and initial characterization of siderophore producing pseudomonads isolated from mine dump and mine drainage

Hofmann

Heine

Schulz

et al. 2020

Biotechnology Reports

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“…The draft genome sequencing of strains Arv20#4.1 and A41C3 was generated on an Illumina platform (MiSeq) as described previously (Retamal-Morales et al, 2018). To this end standard Illumina protocols and kits were employed.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Metabolites Produced Under Iron Limitation Kill Pinewood Nematode and Attract Caenorhabditis elegans

et al. 2019

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Pine Wilt Disease (PWD) is caused by Bursaphelenchus xylophilus, the pinewood nematode, and affects several species of pine trees worldwide. The ecosystem of the Pinus pinaster trees was investigated as a source of bacteria producing metabolites affecting this ecosystem: P. pinaster trees as target-plant, nematode as disease effector and its insect-vector as shuttle. For example, metals and metal-carrying compounds contribute to the complex tree-ecosystems. This work aimed to detect novel secondary metabolites like metallophores and related molecules produced under iron limitation by PWD-associated bacteria and to test their activity on nematodes. After screening 357 bacterial strains from Portugal and United States, two promising metallophore-producing strains Erwinia sp. A41C3 and Rouxiella sp. Arv20#4.1 were chosen and investigated in more detail. The genomes of these strains were sequenced, analyzed, and used to detect genetic potential for secondary metabolite production. A combinatorial approach of liquid chromatography-coupled tandem mass spectrometry (LC-MS) linked to molecular networking was used to describe these compounds. Two major metabolites were detected by HPLC analyses and described. One HPLC fraction of strain Arv20#4.1 showed to be a hydroxamate-type siderophore with higher affinity for chelation of Cu. The HPLC fraction of strain A41C3 with highest metal affinity showed to be a catecholate-type siderophore with higher affinity for chelation of Fe. LC-MS allowed the identification of several desferrioxamines from strain Arv20#4.1, in special desferrioxamine E, but no hit was obtained in case of strain A41C3 which might indicate that it is something new. Bacteria and their culture supernatants showed ability to attract C. elegans. HPLC fractions of those supernatant-extracts of Erwinia strain A41C3, enriched with secondary metabolites such as siderophores, were able to kill pinewood nematode. These results suggest that metabolites secreted under iron limitation have potential to biocontrol B. xylophilus and for management of Pine Wilt Disease.

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Draft genome sequence of Rhodococcus erythropolis B7g, a biosurfactant producing actinobacterium

Cited by 22 publications

References 24 publications

Biotechnology of Rhodococcus for the production of valuable compounds

Biotechnology of Rhodococcus for the production of valuable compounds

Draft genomes and initial characterization of siderophore producing pseudomonads isolated from mine dump and mine drainage

Bacterial Metabolites Produced Under Iron Limitation Kill Pinewood Nematode and Attract Caenorhabditis elegans

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